Harnessing engineered extracellular vesicles for enhanced therapeutic efficacy: advancements in cancer immunotherapy DOI Creative Commons
Zheng Gong, Cheng Cheng, Chaonan Sun

et al.

Journal of Experimental & Clinical Cancer Research, Journal Year: 2025, Volume and Issue: 44(1)

Published: May 2, 2025

Abstract Extracellular vesicles (EVs), particularly engineered variants, have emerged as promising tools in cancer immunotherapy due to their inherent ability modulate immune responses and deliver therapeutic agents with high specificity minimal toxicity. These nanometer-sized vesicles, which include exosomes (Exos) other subtypes, naturally participate intercellular communication are capable of carrying a diverse range bioactive molecules, including proteins, lipids, RNAs, metabolites. Recent advancements the biogenesis EVs, such strategies modify surface characteristics cargo, significantly expanded potential effective vehicles for targeted therapies. Tailoring contents incorporating immunomodulatory molecules or gene-editing (GETs), has shown outcomes enhancing anti-tumor immunity overcoming immunosuppressive tumor microenvironment (TME). Moreover, optimizing delivery mechanisms, through both passive active targeting strategies, is crucial improving clinical efficacy EV-based This review provides an overview recent developments engineering EVs immunotherapy, focusing on biogenesis, methods content customization, innovations cargo delivery. Additionally, addresses challenges associated translation therapies, issues related scalability, safety, By offering insights into current state field identifying key areas future research, this aims advance application treatment.

Language: Английский

The roles and therapeutic potential of exosomal non-coding RNAs in microglia-mediated intercellular communication DOI

Hu-Bo Yang,

Ding-Ci Lu,

Min Shu

et al.

International Immunopharmacology, Journal Year: 2025, Volume and Issue: 148, P. 114049 - 114049

Published: Jan. 16, 2025

Language: Английский

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Drug Delivery Systems Based on Dendritic-Cell-Derived Exosomes DOI Creative Commons
Lihua Chen, Jie Zhang, Yueyan Huang

et al.

Pharmaceutics, Journal Year: 2025, Volume and Issue: 17(3), P. 326 - 326

Published: March 3, 2025

Exosomes, spherical lipid-bilayered particles secreted by cells, have recently emerged as a novel and highly promising drug delivery system, attracting extensive attention in the field of biomedical research. Dendritic-cell-derived exosomes (DC-Exos) possess surface protein ligands characteristic DC such functional MHC-I MHC-II, CD80, CD86. These components play crucial role immune responses, facilitating antigen uptake, presentation, activation antigen-specific CD4 CD8 T cells. properties make them striking excellent vehicles for use various diseases cancer therapy. This review summarizes discusses characteristics, current methods types loading DC-Exos. Its modifications application disease treatment were also discussed, aiming to motivate development exosome-based theranostic nanoplatforms nanotechnology improved healthcare treatments.

Language: Английский

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Extracellular vesicle-mediated bidirectional communication between the liver and other organs: mechanistic exploration and prospects for clinical applications DOI Creative Commons
Wenhui Mo, Yunke Peng,

Yanyi Zheng

et al.

Journal of Nanobiotechnology, Journal Year: 2025, Volume and Issue: 23(1)

Published: March 8, 2025

The liver, functioning as an endocrine organ, secretes a variety of substances that influence the activities other body organs. Conversely, molecules generated by organs such bone, gut, and adipose tissue can also impact liver function. Accumulating evidence suggests bidirectional communication between However, research on how extracellular vesicles (EVs), which transport active molecular mediators, contribute to this interorgan is still in its nascent stages. EVs are capable transporting functional molecules, including lipids, nucleic acids, proteins, thereby affecting recipient cells across different at biological level. This review examines role facilitating cardiovascular system, pancreas, brain, lungs, kidneys, tissue. It explores their potential disease treatment highlights challenges understanding EV-mediated interactions. contribution mediator-carrying two-way remains area ongoing investigation. Future will provide more comprehensive theoretical foundation clarify precise mechanisms governing organs, pinpoint medical targets, expand application within realm precision medicine.

Language: Английский

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Extracellular vesicle as a next-generation drug delivery platform for rheumatoid arthritis therapy DOI
Yi Jin, Cong Xu, Yujuan Zhu

et al.

Journal of Controlled Release, Journal Year: 2025, Volume and Issue: 381, P. 113610 - 113610

Published: March 7, 2025

Language: Английский

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Guidance on the clinical application of extracellular vesicles DOI
Shuji Terai,

Motohiro Asonuma,

Ayuko Hoshino

et al.

Regenerative Therapy, Journal Year: 2025, Volume and Issue: 29, P. 43 - 50

Published: March 7, 2025

Language: Английский

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Microvesicles and exosomes isolated from murine bone marrow-derived mesenchymal stromal cells primed with p38MAPK inhibitor differentially regulate hematopoietic stem cell function DOI Creative Commons
Pallavi Budgude, Vaijayanti Kale, Anuradha Vaidya

et al.

Artificial Cells Nanomedicine and Biotechnology, Journal Year: 2025, Volume and Issue: 53(1), P. 122 - 137

Published: March 10, 2025

The signaling mechanisms active within mesenchymal stromal cells (MSCs) influence the composition of microvesicles (MVs) and exosomes (Exos) secreted by them. Previously, we showed that priming MSCs with a p38 pharmacological inhibitor (pMSCs) rejuvenates them improves their ability to promote ex vivo hematopoietic stem cell (HSC) expansion. This study examined whether pMSCs exerted HSC-supportive via MVs (pMVs) Exos (pExos). Our findings demonstrate distinct regulation HSC fate pMVs pExos. promoted expansion long-term HSCs (LT-HSCs), distinguished robust self-renewal capacity superior engraftment ability. In contrast, pExos facilitated short-term (ST-HSCs) high proliferative differentiation potential. Infusing combination pMVs- pExos-expanded as composite graft resulted in significantly higher engraftment, emphasizing synergistic interaction between LT- ST-HSC populations. Gene expression studies, functional phenotypic experiments regulate quiescence Egr1/Cdkn1a axis, while control proliferation Nfya/Cdkn1a axis. These provide insights into molecular underlying differential function It also proposes strategy using "MSC-derived biologics" for improving transplantation success.

Language: Английский

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Salivary Extracellular Vesicles in Detection of Cancers Other than Head and Neck: A Systematic Review DOI Creative Commons

Wojciech Owecki,

Karolina Wojtowicz, Kacper Nijakowski

et al.

Cells, Journal Year: 2025, Volume and Issue: 14(6), P. 411 - 411

Published: March 11, 2025

Cancer is one of the leading causes death worldwide. Evidence indicates that extracellular vesicles are involved in cancer development and may be used as promising biomarkers detection. Concomitantly, saliva constitutes a non-invasive inexpensive source biomarkers. This systematic review investigates use salivary detecting cancers located outside head neck. PubMed, Web Science, Scopus, Embase were thoroughly searched from database inception to 16 July 2024. Data sixteen eligible studies analyzed, including glioblastoma, lung, esophageal, gastric, prostate, hepatocellular, breast, pancreatobiliary tract cancers. The findings highlight strong diagnostic potential for lung esophageal cancers, where specific exosomal RNAs proteins demonstrated high accuracy distinguishing patients healthy individuals. Additionally, glioblastoma showed prognostic value, while those hepatocellular exhibited early However, gastric prostate limited reliability, breast require further validation. In conclusion, present detection across multiple types; however, their power needs research, standardization large-scale

Language: Английский

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Leveraging engineered yeast small extracellular vesicles serve as multifunctional platforms for effectively loading methyl salicylate through the “esterase-responsive active loading” strategy DOI
Tianhao Li, Yun Zhou, Haoran Wang

et al.

European Journal of Pharmaceutics and Biopharmaceutics, Journal Year: 2025, Volume and Issue: unknown, P. 114696 - 114696

Published: March 1, 2025

Language: Английский

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Exosomes in acute pancreatitis: Pathways to cellular death regulation and clinical application potential DOI

Kaiyi Ruan,

Jinglei Zhang, Zhaowei Chu

et al.

International Immunopharmacology, Journal Year: 2025, Volume and Issue: 153, P. 114491 - 114491

Published: March 21, 2025

Language: Английский

Citations

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A proposed workflow to robustly analyze bacterial transcripts in RNAseq data from extracellular vesicles DOI Creative Commons
Alex M. Ascensión, Miriam Gorostidi-Aicua, Ane Otaegui-Chivite

et al.

Frontiers in Microbiology, Journal Year: 2025, Volume and Issue: 16

Published: March 20, 2025

Introduction The microbiota has been unequivocally linked to various diseases, yet the mechanisms underlying these associations remain incompletely understood. One potential contributor this relationship is extracellular vesicles produced by bacteria (bEVs). However, detection of bEVs challenging. Therefore, we propose a novel workflow identify bacterial RNA present in circulating using Total EV RNA-seq data. As proof concept, applied dataset from individuals with multiple sclerosis (MS). Methods We analyzed total data blood samples healthy controls and MS, encompassing both Relapsing-Remitting (RR) Secondary Progressive (SP) phases disease. Our incorporates reference mapping steps against host genome, followed consensus selection genera based on taxonomic profiling tools. This approach utilizes flagging system exclude low abundance across profilers. Additionally, included EVs derived two cultured species that serve as biological controls, well artificially generated reads 60 technical control, validate specificity workflow. Results findings demonstrate can indeed be detected samples, suggesting powerful tool for reanalyzing studies. identified promising candidates differential expression between RR SP MS. Discussion provides valuable insights into role microbiota-host communication. Finally, translatable other experiments RNA, where lack robust pipeline lead an increased false positive microbial genera. instructions how use it are available at following repository: https://github.com/NanoNeuro/EV_taxprofiling .

Language: Английский

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