Gut Microbes, Journal Year: 2024, Volume and Issue: 16(1)
Published: Oct. 16, 2024
Infection with the protozoan parasite
Language: Английский
Microbial Pathogenesis, Journal Year: 2024, Volume and Issue: 190, P. 106617 - 106617
Published: March 16, 2024
Language: Английский
Citations
9
Cells, Journal Year: 2025, Volume and Issue: 14(2), P. 115 - 115
Published: Jan. 14, 2025
tRNA molecules are among the most fundamental and evolutionarily conserved RNA types, primarily facilitating translation of genetic information from mRNA into proteins. Beyond their canonical role as adaptor during protein synthesis, tRNAs have evolved to perform additional functions. One such non-canonical for is through generation tRNA-derived fragments via specific cleavage processes. These small RNAs (tsRNAs) present across all three domains life, including in protozoan parasites. They formed parent at different sites, resulting either halves or smaller fragments. The precise mechanisms underlying synthesis various fragments, RNases involved, well distinct functions roles parasite physiology, not yet fully understood remain an active area ongoing research. However, modulating gene expression, particularly stress responses, becoming increasingly evident. In this context, we discuss recent findings on Furthermore, investigate how these tsRNAs modulate expression within itself packaged extracellular vesicles alter host thereby promoting survival adaptation.
Language: Английский
Citations
1
Microbiome Research Reports, Journal Year: 2025, Volume and Issue: 4(1)
Published: Feb. 21, 2025
MicroRNAs (miRNAs) are short, non-coding RNAs that play gene expression regulatory roles in eukaryotes. MiRNAs also released body fluids, and the intestine, they found lumen feces. Here, together with exogenous dietary-derived miRNAs, constitute fecal miRNome. Several miRNAs were identified feces of healthy adults, including, as shown here, core hsa-miR-21-5p hsa-miR-1246. These important for intestinal homeostasis. Recent evidence suggests may interact gut bacteria. This represents a new avenue to understand host-bacteria crosstalk its role health disease. review provides comprehensive overview current knowledge on their representation across individuals, effects microbiota. It discusses existing potential mechanisms uptake interaction bacterial genomes, drawing from prokaryotic small (sRNAs) regulation expression. Finally, we silico experimental approaches profiling miRNA-mRNA interactions species, highlighting challenges target validation. work emphasizes need further research into host miRNA-bacterial better ecosystem support exploitation disease prevention treatment.
Language: Английский
Citations
0
Parasites & Vectors, Journal Year: 2025, Volume and Issue: 18(1)
Published: Feb. 24, 2025
Abstract Background Giardia duodenalis is a prevalent intestinal pathogen causing giardiasis, condition characterized by diarrhea and frequently linked to malnutrition growth impairments in children. The virulence of Giardiavirus (GLV) may efficiently clear parasites from infected patients. However, we have limited understanding GLV transmission among species GLV-infected ’s impact on pathogenicity. Methods This study investigated extracellular vesicles (EVs) isolated via ultracentrifugation or exosome assay kit detect the presence EVs, results were detected using ultrastructure molecular methods, including electron microscopy, scanning quantitative polymerase chain reaction (qPCR), dot blot. Transwell migration assays confirmed spread GLV-enveloped EVs inhibitor experiments immunofluorescence. Mice gavaged with , without infection, assessed for disease progression, parameters (weight size gains), permeability, pathology. Results Parts exploit pathway reach environment, allowing these EVs. uptake GLV-containing rapid trophozoite wrapped also offers protection against external interference. Importantly, EV-coated leads divergent clinical symptoms mice, posing less risk mice reducing symptoms, such as emaciation, stunted growth, lesion damage, compared GLV-free -infected mice. Conclusions Our studies show that can species, infection alleviates lesions caused . These findings reveal could be target development novel intervention strategies Graphical abstract
Language: Английский
Citations
0
Animals and zoonoses., Journal Year: 2025, Volume and Issue: unknown
Published: March 1, 2025
Language: Английский
Citations
0
Aggregate, Journal Year: 2025, Volume and Issue: unknown
Published: March 27, 2025
ABSTRACT Extracellular vesicles (EVs) are essential for host–pathogen interactions, mediating processes such as immune modulation and pathogen survival. Pathogen‐derived EVs hold significant diagnostic potential because of their unique cargo, offering a wealth biomarkers. In this review, we first discuss the roles derived from various pathogens in interactions summarize latest advancements detection based on EVs. Then, highlight innovative strategies, including novel aggregate materials machine learning approaches, enhancing EV analysis. Finally, challenges field future directions advancing EV‐based diagnostics, aiming to translate these insights into clinical applications.
Language: Английский
Citations
0
mBio, Journal Year: 2025, Volume and Issue: unknown
Published: April 29, 2025
ABSTRACT Extracellular vesicles (EVs) are membrane-enclosed nanoparticles that contain proteins, lipids, and nucleic acids, playing key roles in interactions between pathogens hosts. Most research on pathogen-derived EVs has focused understanding their impact disease pathogenesis, immunomodulation, use as biomarkers for diagnosis. However, few studies have explored the cross talk bacteria, fungi, protozoa, helminths, or arthropods via EVs. This is particularly relevant human gut microenvironment, where a high diversity of microbes exists modulated with helminth gastrointestinal infections. Additionally, during blood-borne coinfections like malaria lymphatic filariasis, direct communication may take place, arthropod-pathogen interface, multiplication some protozoa helminths essential development. Understanding transmicrobe EV reveal novel therapeutic strategies controlling infectious diseases both vertebrate invertebrate hosts, regions coinfection rates.
Language: Английский
Citations
0
Pflügers Archiv - European Journal of Physiology, Journal Year: 2024, Volume and Issue: 476(9), P. 1353 - 1368
Published: April 4, 2024
Mammalian cells utilize glucose as a primary carbon source to produce energy for most cellular functions. However, the bioenergetic homeostasis of can be perturbed by environmental alterations, such changes in oxygen levels which associated with bacterial infection. Reduction availability leads state hypoxia, inducing numerous responses that aim combat this stress. Importantly, hypoxia strongly augments glycolysis cell types compensate loss aerobic respiration. Understanding how host metabolic adaptation impacts course infection will identify new anti-microbial targets. This review highlight developments our understanding glycolytic substrate channeling and spatiotemporal enzymatic organization response shedding light on integral role hypoxia-inducible factor (HIF) during host-pathogen interactions. Furthermore, ability intracellular extracellular bacteria (pathogens commensals alike) modulate metabolism discussed.
Language: Английский
Citations
1
Gut Microbes, Journal Year: 2024, Volume and Issue: 16(1)
Published: Oct. 16, 2024
Infection with the protozoan parasite
Language: Английский
Citations
0