Bone Marrow Mesenchymal Stem Cells–Derived Extracellular Vesicle miR-208a-3p Alleviating Spinal Cord Injury via Regulating the Biological Function of Spinal Cord Neurons DOI

Jianwei Yang,

Yanhua Yao

DNA and Cell Biology, Journal Year: 2024, Volume and Issue: unknown

Published: Aug. 12, 2024

We aim to explore the potential mechanism of bone marrow mesenchymal stem cells-derived extracellular vesicles (BMSCs-Exo) in improving spinal cord injury (SCI). Thirty male 12-week specific pathogen-free (SPF) Sprague-Dawley (SD) rats were used construct SCI model

Language: Английский

Extracellular vesicles DOI

Mariana Ottaiano Gonçalves,

Juliana Fortes Di Iorio,

Gabriela Villa Marin

et al.

Current topics in membranes, Journal Year: 2024, Volume and Issue: unknown, P. 1 - 31

Published: Jan. 1, 2024

Language: Английский

Citations

1
MiR-29a-laden extracellular vesicles efficiently induced apoptosis through autophagy blockage in HCC cells DOI

Homeyra Seydi,

Kosar Nouri,

Bahare Shokouhian

et al.

European Journal of Pharmaceutics and Biopharmaceutics, Journal Year: 2024, Volume and Issue: 203, P. 114470 - 114470

Published: Aug. 26, 2024

Language: Английский

Citations

1
Extracellular vesicles from human cardiac stromal cells up-regulate cardiomyocyte protective responses to hypoxia DOI Creative Commons
Andreas Czosseck, Max M. Chen, Chuan-Chih Hsu

et al.

Stem Cell Research & Therapy, Journal Year: 2024, Volume and Issue: 15(1)

Published: Oct. 12, 2024

Abstract Background Cell therapy can protect cardiomyocytes from hypoxia, primarily via paracrine secretions, including extracellular vesicles (EVs). Since EVs fulfil specific biological functions based on their cellular origin, we hypothesised that human cardiac stromal cells (CMSCLCs) obtained coronary artery bypass surgery may have cardioprotective properties. Objectives This study characterises CMSCLC (C_EVs), miRNA cargo, efficacy and transcriptomic modulation of hypoxic induced pluripotent stem cell-derived (iPSC-CMs). C_EVs are compared to bone marrow mesenchymal cell (B_EVs) which a known therapeutic EV type. Methods Cells were characterised for surface markers, gene expression differentiation potential. yield, phenotype, ability hiPSC-CMs hypoxia/reoxygenation injury. dose was normalised by both protein concentration particle count, allowing direct comparison. C_EV B_EV cargo profiled RNA-seq performed EV-treated hiPSC-CMs, then data integrated multi-omics. Confirmatory experiments carried out using mimics. Results At the same dose, more effective than B_EVs at protecting CM integrity, reducing apoptotic death during hypoxia. While shared 70–77% similarity in content, contained unique miRNAs, miR-202-5p, miR-451a miR-142-3p. Delivering mimics confirmed miR-1260a miR-202/451a/142 cardioprotective, latter upregulated protective pathways similar whole C_EVs. Conclusions demonstrates potential tissues, routinely discarded following surgery, as valuable source myocardial infarction therapy. We also identify

Language: Английский

Citations

1
A call for standardization for secretome and extracellular vesicles in osteoarthritis: results show disease-modifying potential, but protocols are too heterogeneous—a systematic review DOI
Daniele D’Arrigo, Manuela Salerno, Luca De Marziani

et al.

Human Cell, Journal Year: 2024, Volume and Issue: 37(5), P. 1243 - 1275

Published: June 23, 2024

Language: Английский

Citations

0
Bone Marrow Mesenchymal Stem Cells–Derived Extracellular Vesicle miR-208a-3p Alleviating Spinal Cord Injury via Regulating the Biological Function of Spinal Cord Neurons DOI

Jianwei Yang,

Yanhua Yao

DNA and Cell Biology, Journal Year: 2024, Volume and Issue: unknown

Published: Aug. 12, 2024

We aim to explore the potential mechanism of bone marrow mesenchymal stem cells-derived extracellular vesicles (BMSCs-Exo) in improving spinal cord injury (SCI). Thirty male 12-week specific pathogen-free (SPF) Sprague-Dawley (SD) rats were used construct SCI model

Language: Английский

Citations

0