Rapid isolation of extracellular vesicles from stem cell conditioned medium using osmosis-driven filtration DOI Creative Commons

Casey Y. Huang,

Helen Nguyễn, David J. Lundy

et al.

Science and Technology of Advanced Materials, Journal Year: 2025, Volume and Issue: unknown

Published: April 3, 2025

Extracellular vesicles (EVs) hold significant promise as biomarkers and therapeutics, yet their isolation remains challenging due to low abundance complex sample matrices. Here, we introduce EV-Osmoprocessor (EVOs), a novel device that leverages osmosis-driven filtration for rapid efficient EV isolation. EVOs employs high osmolarity polymer solution concentrate EVs while simultaneously removing smaller contaminants. Compared traditional methods such ultracentrifugation precipitation, offers speed convenience, achieving 50-fold volume reduction in under 2 h. Our results show retained removed >99% albumin from the cell conditioned culture medium (CCM). The isolated exhibited particle size distribution centered around 140 nm, which was very similar via precipitation or ultracentrifugation. standalone process achieved particle:protein ratio (EV purity) of ~107 particles/µg protein. Comprehensive characterization, including cryo-electron microscopy, validation protein markers known miRNA cargo confirmed successful EVs. Functional assays, based on protection cardiomyocytes hypoxia/reoxygenation injury, demonstrated bioactivity EVOs-isolated Furthermore, can be used 30 ml CCM into 0.5 solution, then further processed with size-exclusion chromatography (SEC), improving purity ~109 This work establishes promising tool research clinical applications, offering streamlined approach enhanced analytical performance.

Language: Английский

From Bench to Bedside: The Evolution of Extracellular Vesicle Diagnostics through Microfluidic and Paper-Based Technologies DOI
P. Gopalan,

Arjun Jayachandran,

Sasidhar Manda Venkata

et al.

Colloids and Surfaces B Biointerfaces, Journal Year: 2025, Volume and Issue: unknown, P. 114675 - 114675

Published: April 1, 2025

Language: Английский

Citations

0
Rapid isolation of extracellular vesicles from stem cell conditioned medium using osmosis-driven filtration DOI Creative Commons

Casey Y. Huang,

Helen Nguyễn, David J. Lundy

et al.

Science and Technology of Advanced Materials, Journal Year: 2025, Volume and Issue: unknown

Published: April 3, 2025

Extracellular vesicles (EVs) hold significant promise as biomarkers and therapeutics, yet their isolation remains challenging due to low abundance complex sample matrices. Here, we introduce EV-Osmoprocessor (EVOs), a novel device that leverages osmosis-driven filtration for rapid efficient EV isolation. EVOs employs high osmolarity polymer solution concentrate EVs while simultaneously removing smaller contaminants. Compared traditional methods such ultracentrifugation precipitation, offers speed convenience, achieving 50-fold volume reduction in under 2 h. Our results show retained removed >99% albumin from the cell conditioned culture medium (CCM). The isolated exhibited particle size distribution centered around 140 nm, which was very similar via precipitation or ultracentrifugation. standalone process achieved particle:protein ratio (EV purity) of ~107 particles/µg protein. Comprehensive characterization, including cryo-electron microscopy, validation protein markers known miRNA cargo confirmed successful EVs. Functional assays, based on protection cardiomyocytes hypoxia/reoxygenation injury, demonstrated bioactivity EVOs-isolated Furthermore, can be used 30 ml CCM into 0.5 solution, then further processed with size-exclusion chromatography (SEC), improving purity ~109 This work establishes promising tool research clinical applications, offering streamlined approach enhanced analytical performance.

Language: Английский

Citations

0