bioRxiv (Cold Spring Harbor Laboratory),
Journal Year:
2023,
Volume and Issue:
unknown
Published: Aug. 2, 2023
Abstract
Cancer
spheroids
offer
a
valuable
experimental
model
that
mimics
the
complexity
and
heterogeneity
of
solid
tumors.
Characterizing
their
mechanical
response
is
crucial
for
understanding
tumor
development,
progression,
drug
response.
Currently,
whole
live
are
analyzed
primarily
using
image
analysis,
which
challenging,
requires
extended
incubation
times,
has
limited
imaging
depth.
Here,
we
present
new
label-free
approach
characterizing
sub-superficial
structures
bladder
cancer
measuring
at
three
distinct
stages
progression.
We
study
microrheological
changes
induced
by
aging
cellular
cluster
levels
conducting
multi-physics
characterization
modeling
approach.
find
exhibit
viscoelastic
behavior
can
be
described
fractional
models.
show
mechanically
heterogeneous,
with
strong
depth
time-dependent
variations
associated
evolving
structural
features.
Our
opens
possibilities
to
3D
in
vitro
models,
paving
way
discovery
novel
more
precise
procedure
diagnosis
based
on
use
mechanomarkers.
Annual Review of Biophysics,
Journal Year:
2024,
Volume and Issue:
53(1), P. 367 - 395
Published: Feb. 21, 2024
The
mechanical
phenotype
of
a
cell
determines
its
ability
to
deform
under
force
and
is
therefore
relevant
cellular
functions
that
require
changes
in
shape,
such
as
migration
or
circulation
through
the
microvasculature.
On
practical
level,
can
be
used
global
readout
cell's
functional
state,
marker
for
disease
diagnostics,
an
input
tissue
modeling.
We
focus
our
review
on
current
knowledge
structural
components
contribute
determination
properties
highlight
physiological
processes
which
cells
critical
relevance.
ongoing
efforts
understand
how
efficiently
measure
control
will
define
progress
field
drive
phenotyping
toward
clinical
applications.
Analytica Chimica Acta,
Journal Year:
2025,
Volume and Issue:
1355, P. 343894 - 343894
Published: March 4, 2025
Shear
flow
deformability
cytometry
is
an
emerging
microfluidic
technique
that
has
undergone
significant
advances
in
the
last
few
years
and
offers
considerable
potential
for
clinical
diagnostics
disease
monitoring.
By
simultaneously
measuring
mechanical
morphological
parameters
of
single
cells,
it
a
comprehensive
extension
traditional
cell
analysis,
delivering
unique
insight
into
deformability,
which
gaining
recognition
as
novel
biomarker
health
disease.
Due
to
its
operating
principle,
method
particularly
suitable
analysis
blood
samples.
This
review
focuses
on
recent
developments
shear
cytometry,
widely
adopted
variant
cytometry.
It
strong
applications
practice
due
robust
simple
operation,
demonstrated
with
whole
samples,
well
high
throughput,
can
reach
approximately
1000
cells
per
second.
We
begin
by
discussing
some
basic
factors
influence
properties
give
overview
operational
principles
samples
from
blood,
cultured
tissues.
Next,
we
clinically
relevant
cancer
cells.
Finally,
address
key
challenges
adoption,
such
regulatory
approval,
scalable
manufacturing,
workflow
integration,
emphasizing
need
further
validation
studies
facilitate
implementation.
article
uniquely
emphasizes
relevance
giving
biomarkers
studied
In
addition,
addresses
critical
barriers
translation.
identifying
these
obstacles,
this
aims
demonstrate
bridge
gap
between
research
routine
medical
practice.
Journal of The Royal Society Interface,
Journal Year:
2025,
Volume and Issue:
22(224)
Published: March 1, 2025
Changes
in
the
mechanical
properties
of
extracellular
matrix
(ECM)
are
a
hallmark
disease.
Due
to
its
relevance,
several
vitro
models
have
been
developed
for
ECM,
including
cell-derived
matrices
(CDMs).
CDMs
decellularized
natural
ECMs
assembled
by
cells
that
closely
mimic
vivo
stromal
fibre
organization
and
molecular
content.
Here,
we
applied
atomic
force
microscopy-force
spectroscopy
(AFM-FS)
evaluate
nanomechanical
obtained
from
patients
diagnosed
with
collagen
VI-related
congenital
muscular
dystrophies
(COL6-RDs).
COL6-RDs
set
neuromuscular
conditions
caused
pathogenic
variants
any
three
major
COL6
genes,
which
result
deficiency
or
dysfunction
incorporated
into
ECM
connective
tissues.
Current
diagnosis
includes
genetic
confirmation
disease
categorization
phenotype
based
on
maximum
motor
ability,
as
no
direct
correlation
exists
between
genotype
COL6-RDs.
We
describe
differences
elastic
modulus
(
E
)
among
different
clinical
phenotypes,
well
restoration
genetically
edited
cells.
Results
anticipate
potential
analysis
complementary
tool,
providing
phenotypic
information
about
their
response
gene
therapies.
Scientific Reports,
Journal Year:
2024,
Volume and Issue:
14(1)
Published: Oct. 28, 2024
Atomic
Force
Microscopy
(AFM)
is
a
leading
nanoscale
technique
known
for
its
significant
advantages
in
the
analysis
of
soft
materials
and
biological
samples.
Traditional
AFM
data
often
based
on
Hertz
model,
which
assumes
perpendicular
indentation
planar
sample.
However,
this
assumption
not
always
valid
due
to
varying
geometries
materials,
whether
natural,
synthetic
or
biological.
In
study,
we
present
new
theoretical
model
that
incorporates
correction
coefficients
into
Hertz's
account
cone-like
spherical
probes,
consider
local
tilt
at
probe-sample
interface.
We
validate
our
using
finite
element
(FEA)
simulations
experimental
measurements
tilted
polyacrylamide
gels.
Our
results
highlight
need
include
contact
ensure
accurate
measurements.
This
represents
step
forward
understanding
elastic
properties
surface
broadest
sense.
Pharmaceutics,
Journal Year:
2024,
Volume and Issue:
16(6), P. 733 - 733
Published: May 29, 2024
Cell
mechanics
is
gaining
attraction
in
drug
screening,
but
the
applicable
methods
have
not
yet
become
part
of
standardized
norm.
This
review
presents
current
state
art
for
atomic
force
microscopy,
which
most
widely
available
method.
The
field
first
motivated
as
a
new
way
tracking
pharmaceutical
effects,
followed
by
basic
introduction
targeted
at
pharmacists
on
how
to
measure
cellular
stiffness.
then
moves
knowledge
terms
experimental
results
and
supplementary
such
fluorescence
microscopy
that
can
give
relevant
additional
information.
Finally,
rheological
approaches
well
theoretical
interpretations
are
presented
before
ending
outlooks.
Research Square (Research Square),
Journal Year:
2024,
Volume and Issue:
unknown
Published: Aug. 12, 2024
Abstract
In
the
field
of
cellular
health
assessment,
mechanical
properties
cells
are
crucial
indicators.
Atomic
Force
Microscopy
(AFM)
is
a
prominent
nanoscale
technique
used
for
its
significant
benefits
in
analyzing
cell
mechanics.
Traditional
analysis
AFM
data
often
relies
on
Hertz's
law,
which
assumes
flat
surface
biological
sample.
However,
this
assumption
does
not
always
hold
true
due
to
diverse
geometries
cells.
study,
we
present
new
theoretical
model
that
includes
correction
coefficients
law
consider
cone-like
and
spherical
probes,
addressing
local
tilt
at
probe-sample
interface.
We
validated
our
through
Finite
Element
Analysis
(FEA)
simulations
experimental
measurements
tilted
polyacrylamide
gels.
Our
findings
emphasize
importance
accounting
contact
ensure
accurate
measurements.
This
marks
advancement
understanding
mechanics
nanoscale.
Computational and Structural Biotechnology Journal,
Journal Year:
2024,
Volume and Issue:
24, P. 661 - 671
Published: Oct. 5, 2024
The
influence
of
biomechanics
on
cell
function
has
become
increasingly
defined
over
recent
years.
Biomechanical
changes
are
known
to
affect
oncogenesis;
however,
these
effects
not
yet
fully
understood.
Atomic
force
microscopy
(AFM)
is
the
gold
standard
method
for
measuring
tissue
mechanics
micro-
or
nano-scale.
Due
its
complexity,
AFM
integrated
in
routine
clinical
diagnosis.
Artificial
intelligence
(AI)
and
machine
learning
(ML)
have
potential
make
more
accessible,
principally
through
automation
analysis.
In
this
review,
use
assessment
cancer
described.
Research
relating
application
artificial
analysis
topography
spectroscopy
cells
reviewed.
enable
widespread
nanoscale
morphologic
biomechanical
features
as
diagnostic
prognostic
biomarkers
treatment.
