Ultrasensitive Lateral Flow Immunoassay of Fluoroquinolone Antibiotic Gatifloxacin Using Au@Ag Nanoparticles as a Signal-Enhancing Label DOI Creative Commons
Olga D. Hendrickson, Nadezhda A. Byzova, Vasily G. Panferov

et al.

Biosensors, Journal Year: 2024, Volume and Issue: 14(12), P. 598 - 598

Published: Dec. 6, 2024

Gatifloxacin (GAT), an antibiotic belonging to the fluoroquinolone (FQ) class, is a toxicant that may contaminate food products. In this study, method of ultrasensitive immunochromatographic detection GAT was developed for first time. An indirect format lateral flow immunoassay (LFIA) performed. GAT-specific monoclonal antibodies and labeled anti-species were used in LFIA. Bimetallic core@shell Au@Ag nanoparticles (Au@Ag NPs) synthesized as new label. Peroxidase-mimic properties NPs allowed catalytic enhancement signal on test strips, increasing assay sensitivity. A mechanism NPs-mediated catalysis deduced. Signal amplification achieved through oxidative etching by hydrogen peroxide. This resulted formation gold Ag+ ions, which catalyzed oxidation peroxidase substrate. Such “chemical enhancement” reaching instrumental limit (LOD, calculated Three Sigma approach) cutoff 0.8 20 pg/mL, respectively. The enhanced procedure can be completed 21 min. LFIA tested raw meat samples, recoveries from 78.1–114.8%. recommended promising instrument sensitive various toxicants.

Language: Английский

Formulation Advances in Posterior Segment Ocular Drug Delivery DOI
Tumpa Sarkar, Niva Rani Gogoi, Bani Kumar Jana

et al.

Journal of Ocular Pharmacology and Therapeutics, Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 23, 2025

Posterior segment ocular diseases, such as diabetic retinopathy, age-related macular degeneration, and retinal vein occlusion, are leading causes of vision impairment blindness worldwide. Effective management these conditions remains a formidable challenge due to the unique anatomical physiological barriers eye, including blood-retinal barrier rapid drug clearance mechanisms. To address hurdles, nanostructured delivery systems proposed overcome barriers, target retina, enhance permeation while ensuring controlled release. Traditional therapeutic approaches, intravitreal injections, pose significant drawbacks, patient discomfort, poor compliance, potential complications. Therefore, understanding physiology mechanism eye could aid in design novel formulations that be noninvasive deliver drugs reach site is pivotal for effective treatment strategies. This review focuses on recent advances formulation strategies posterior delivery, highlighting their limitations. Furthermore, nanocarrier in-situ gel, niosomes, hydrogels, dendrimers, liposomes, nanoparticles, nanoemulsions more effectively selectively explored, supplemented with illustrative examples, figures, tables. aims provide insights into current state emphasizing need interdisciplinary approaches develop patient-centric, minimally invasive, solutions.

Language: Английский

Citations

1

Ultrasensitive Lateral Flow Immunoassay of Fluoroquinolone Antibiotic Gatifloxacin Using Au@Ag Nanoparticles as a Signal-Enhancing Label DOI Creative Commons
Olga D. Hendrickson, Nadezhda A. Byzova, Vasily G. Panferov

et al.

Biosensors, Journal Year: 2024, Volume and Issue: 14(12), P. 598 - 598

Published: Dec. 6, 2024

Gatifloxacin (GAT), an antibiotic belonging to the fluoroquinolone (FQ) class, is a toxicant that may contaminate food products. In this study, method of ultrasensitive immunochromatographic detection GAT was developed for first time. An indirect format lateral flow immunoassay (LFIA) performed. GAT-specific monoclonal antibodies and labeled anti-species were used in LFIA. Bimetallic core@shell Au@Ag nanoparticles (Au@Ag NPs) synthesized as new label. Peroxidase-mimic properties NPs allowed catalytic enhancement signal on test strips, increasing assay sensitivity. A mechanism NPs-mediated catalysis deduced. Signal amplification achieved through oxidative etching by hydrogen peroxide. This resulted formation gold Ag+ ions, which catalyzed oxidation peroxidase substrate. Such “chemical enhancement” reaching instrumental limit (LOD, calculated Three Sigma approach) cutoff 0.8 20 pg/mL, respectively. The enhanced procedure can be completed 21 min. LFIA tested raw meat samples, recoveries from 78.1–114.8%. recommended promising instrument sensitive various toxicants.

Language: Английский

Citations

1