Neither cholelithiasis nor cholecystectomy is causally associated with colorectal cancer: A univariate and multivariate mendelian randomization study DOI Creative Commons
Qiong Qin, Yong Zhou, Ao Ren

et al.

Research Square (Research Square), Journal Year: 2024, Volume and Issue: unknown

Published: Oct. 30, 2024

Abstract Background Epidemiological studies reported controversial results regarding the relationship between cholelithiasis, cholecystectomy and colorectal cancer (CRC). In presence of reverse causality confounding factors, findings our previous retrospective study that it was gallbladder disease rather than a risk factor for not sufficiently convincing. Therefore, we used Mendelian randomization (MR) to further explore cholelithiasis or CRC. Methods We performed univariate MR (UVMR) multivariate (MVMR) analysis 1,054,773 samples 37,970,958 SNPs from three European genome-wide association (GWAS) The forward analysis, with as exposure CRC outcome, included UVMR MVMR analysis. UVMR, 21 30 independent strongly (P < 5*10− 8) associated on were extracted valid instrumental variables (IVs); in MVMR, 14 26 IVs extracted, respectively. inverse only MVMR. 9 7 estimated using multiplicative random effects-inverse variance weighted (MRE-IVW), simple mode (SM), median (WME), (WMO) MR-Egger regression methods, Sensitivity heterogeneity test, pleiotropy test leave-one-out test. presented scatter plots, forest plots funnel plots. Results neither nor by MRE-IVW revealed causal (OR = 1.0002, 95% CI 0.999–1.001, P 0.729 OR 1.0003, 0.998–1.003, 0.799, respectively) 0.9917, 0.963–1.022, 0.582 0.9897, 0.936–1.046, 0.715, respectively). estimates also little evidence 0.0504, 0.001–2.871, 0.147) 0.9894, 0.792–1.236, 0.925). other estimation methods consistent MRE-IVW. confirmed stability reliability results. Conclusions Our two-sample causally cancer.

Language: Английский

An Investigative Study of LGALSL and HLA‐DRB1 as Prognostic Biomarkers and Therapeutic Targets in Chronic Hepatitis B Patients With Persistent HBV DNA Viremia Under Entecavir Treatment DOI
Qun Wan, Xiaolin Yu, Jinyu Huang

et al.

Journal of Medical Virology, Journal Year: 2025, Volume and Issue: 97(4)

Published: April 1, 2025

Despite significant advances in chronic hepatitis B (CHB) treatment, some patients receiving entecavir (ETV) still experience poor clinical outcomes. Identifying host factors contributing to ETV anti-HBV failure CHB with persistent HBV DNA positivity is crucial for developing targeted therapies. We conducted a comprehensive study using univariate and reverse Mendelian randomization (MR), incorporating sequencing data publicly available genetic data, followed by gene set variation analysis (GSVA), enrichment (GSEA) immune cell infiltration systematically explore causal associations between CHB. Univariate MR analyses revealed inverse association increased HLA-DRB1 levels risk (odds ratio [OR] 0.607, 95% confidence interval [CI] 0.478-0.771, p = 0.00004), while LGALSL were significantly associated heightened of prognosis (OR 1.110, CI: 1.017-1.212, 0.01885), as estimated the variance weighting (IVW) method. Analysis showed higher HLA mast group. positive correlation HLA, whereas negative correlation. Compared favorable prognoses, those prognoses exhibited serum (ELISA), lower expression peripheral blood mononuclear cells (PBMCs) (qPCR), liver tissue (IHC). Therefore, may serve potential prognostic biomarkers ETV, providing novel avenues diagnosis treatment.

Language: Английский

Citations

0
Decoding the causal association between immune cells and three chronic respiratory diseases: Insights from a bi-directional Mendelian randomization study DOI Creative Commons
Anqi Xie, He Zhao, Chenghu Song

et al.

BMC Pulmonary Medicine, Journal Year: 2025, Volume and Issue: 25(1)

Published: April 15, 2025

Numerous studies have indicated the correlations of immune traits and chronic respiratory diseases (CRDs). Whereas, causality is still implicative. Hence, our study was designed to investigate causal relations utilizing bidirectional Mendelian randomization (MR) identify potential significance. Using GWAS datasets, we performed analyses examine 731 associated with three CRDs: asthma, bronchiectasis obstructive pulmonary disease (COPD). Six widely applied MR approaches, along Bayesian weighted analysis, were utilized assess causality. Through extensive sensitivity assessments, heterogeneity pleiotropy been examined. For integrity, leave-one-out analysis implemented as final step. Our reveals 13 that may a genetic basis for predicting occurrence CRDs, which include two risk (CD62L- myeloid dendritic cell (DC) absolute count (AC), CD8 on CD28+ CD45RA- CD8+ T cell) four protective (CD39+ %T cell, CD4 CD39+ activated regulatory (Treg) herpes virus entry mediator (HVEM) Central Memory (CM) CD16 CD14+ CD16+ monocyte) in COPD, (IgD- CD27- %B CD3 CM one trait %DC) bronchiectasis. Additionally, (CD14- CD16- AC monocyte, CD19 IgD+ CD38+ B (HVEM CD4+ identified asthma. Sensitivity showed no indications or signs heterogeneity. The inverse assessment results gave evidence reverse causations, ultimately validating soundness findings. investigation identifies latent major offering novel perspectives preventive therapeutical strategies CRDs.

Language: Английский

Citations

0
Causal effect of serum matrix metalloproteinase levels on venous thromboembolism: a Mendelian randomization study DOI Creative Commons

Deheng Han,

Fangcong Yu,

Liangrong Zheng

et al.

Epidemiology and Health, Journal Year: 2024, Volume and Issue: unknown, P. e2024046 - e2024046

Published: April 24, 2024

Serum matrix metalloproteinase (MMP) levels are associated with cardiovascular diseases. However, the causal associations between serum of specific MMPs and venous thromboembolism (VTE) remain unclear. The present study sought to explore relationship MMP VTE by using Mendelian randomization (MR) method.

Language: Английский

Citations

1
Revealing an association between HPV and systemic lupus erythematosus: A bidirectional two‐sample Mendelian randomization study DOI Creative Commons
Fangfang Pan, Huiliang Shen,

Ben Wang

et al.

Skin Research and Technology, Journal Year: 2024, Volume and Issue: 30(8)

Published: Aug. 1, 2024

Abstract Background An increasing number of studies have focused on the association between Human papillomavirus (HPV) infection and systemic lupus erythematosus (SLE). However, current evidence is largely based retrospective studies, which are susceptible to confounding factors cannot establish causation. Methods A bidirectional two‐sample Mendelian randomization (MR) design was used evaluate causal relationship HPV SLE. Mononucleoside polymers (SNPS) with strong for genome‐wide (GWAS) were selected from exposure dataset as an instrumental variable (IV) this study. For MR Analysis results, MR‐Egger intercept P test, MR‐Presso global CochranQ test leave‐one sensitivity analysis. Results Based Analysis, study finally determined that there no HPV16 HPV18 Conclusions Possible regulation not significantly associated These findings provide new insights into underlying mechanisms SLE need be validated by further studies.

Language: Английский

Citations

1
Neither cholelithiasis nor cholecystectomy is causally associated with colorectal cancer: A univariate and multivariate mendelian randomization study DOI Creative Commons
Qiong Qin, Yong Zhou, Ao Ren

et al.

Research Square (Research Square), Journal Year: 2024, Volume and Issue: unknown

Published: Oct. 30, 2024

Abstract Background Epidemiological studies reported controversial results regarding the relationship between cholelithiasis, cholecystectomy and colorectal cancer (CRC). In presence of reverse causality confounding factors, findings our previous retrospective study that it was gallbladder disease rather than a risk factor for not sufficiently convincing. Therefore, we used Mendelian randomization (MR) to further explore cholelithiasis or CRC. Methods We performed univariate MR (UVMR) multivariate (MVMR) analysis 1,054,773 samples 37,970,958 SNPs from three European genome-wide association (GWAS) The forward analysis, with as exposure CRC outcome, included UVMR MVMR analysis. UVMR, 21 30 independent strongly (P < 5*10− 8) associated on were extracted valid instrumental variables (IVs); in MVMR, 14 26 IVs extracted, respectively. inverse only MVMR. 9 7 estimated using multiplicative random effects-inverse variance weighted (MRE-IVW), simple mode (SM), median (WME), (WMO) MR-Egger regression methods, Sensitivity heterogeneity test, pleiotropy test leave-one-out test. presented scatter plots, forest plots funnel plots. Results neither nor by MRE-IVW revealed causal (OR = 1.0002, 95% CI 0.999–1.001, P 0.729 OR 1.0003, 0.998–1.003, 0.799, respectively) 0.9917, 0.963–1.022, 0.582 0.9897, 0.936–1.046, 0.715, respectively). estimates also little evidence 0.0504, 0.001–2.871, 0.147) 0.9894, 0.792–1.236, 0.925). other estimation methods consistent MRE-IVW. confirmed stability reliability results. Conclusions Our two-sample causally cancer.

Language: Английский

Citations

0