A strategy for synergistic enhancement of immune circulation in head and neck squamous cell carcinoma by novel nucleic acid drug therapy and immunotherapy

Journal of Translational Medicine, Journal Year: 2025, Volume and Issue: 23(1)

Published: March 20, 2025

Studies have shown that in the pathogenesis of head and neck squamous cell carcinoma, immune circulation obstruction caused by various factors including metabolic abnormalities, gene mutations, matrix barrier, is a critical factor for induction tumor development progression. Therefore, immunotherapy strategy killing carcinoma cells an enhanced mechanism has attracted much attention. In addition, rapid new nucleic acid drug therapy, such as mRNA, oligonucleotide small guide RNA (sgRNA), taken (immune checkpoint inhibitors, vaccines, cellular immunotherapy, cytokines adjuvants, etc.) to level. The combination therapy with developed its therapeutic properties brought direction diagnosis treatment two had considerable curative effect patients refractory/recurrent carcinoma. this review, we summarized latest progress applied conventional discussed action efficacy, looked into future trend.

Language: Английский

---

Detection of HPV DNA in Saliva of Patients with HPV-Associated Oropharyngeal Cancer Treated with Radiotherapy

Current Oncology, Journal Year: 2024, Volume and Issue: 31(8), P. 4397 - 4405

Published: Aug. 1, 2024

Background: To investigate the technical feasibility of RT–PCR and direct sequencing to quantify HPV DNA in saliva patients with Human-Papilloma-Virus related oropharyngeal cancer (HPV-OPC), level which is known predict prognosis after treatment. Methods: Nine locally advanced HPV-OPC treated definitive radiotherapy chemotherapy or cetuximab, alone between April 2016 September 2017, were enrolled, two whom also received induction chemotherapy. Saliva was collected before (baseline), during (mid-RT) (post-RT) radiotherapy. HPV-16 DNAs (E6 E7) quantified by sequencing, latter using a custom panel. Correlations levels clinical outcomes assessed. Results: Compared baseline, relative cycle threshold (Ct) value E6 E7 reduced at point mid-RT majority (100% 75% for E7, respectively). Similarly, Ct from baseline post-RT 86% 100% respectively. During follow-up period, three (33%) experienced disease progression. The values these top 4 all patients. sequences detected five (83%) six samples that underwent along several gene mutations, such as TP53,CDKN2A PIK3CA. Conclusions: This study demonstrates that, addition detection quantification RT–PCR, HPV-DNA customized panel technically possible.

Language: Английский

---

Circulating tumor DNA in head and neck squamous cell carcinoma—current status and future prospects

Academia oncology., Journal Year: 2024, Volume and Issue: 1(2)

Published: Dec. 19, 2024

Squamous cell carcinoma (SCC) is the most common malignancy of head and neck. Stagnating survival rates in recent decades, despite advances treatment paradigms, surveillance technologies, multidisciplinary care, leave clinicians with a need for better options screening, risk-stratifying, monitoring patients. A growing proportion patients HPV-associated SCC have improved outcomes but continue to heterogenous response treatment. Advances platforms assays measuring circulating tumor DNA offer an opportunity monitor disease status at molecular level both virally mediated traditional risk-factor-driven This overview will discuss experimental, clinically used, commercially available liquid biopsy their applications neck malignancies.

Language: Английский

---

AVANÇOS NO DIAGNÓSTICO MOLECULAR DO PAPILOMA VÍRUS HUMANO E SUA IMPORTÂNCIA CLÍNICA

Revista Contemporânea, Journal Year: 2024, Volume and Issue: 4(12), P. e7139 - e7139

Published: Dec. 31, 2024

Introdução: A infecção pelo Papilomavírus Humano (HPV) possui alta transmissibilidade e atua no desenvolvimento de lesões cérvico-vaginais, bem como câncer do colo útero. Assim, entende-se que o problema da HPV Brasil representa um importante saúde pública novas discussões acerca desta problemática, são necessárias enorme relevância. Este estudo tem por objetivo descrever os avanços diagnóstico molecular Humano, identificando métodos diagnósticos moleculares mais atuais correlacionando com a importância clínica. Metodologia: Será realizada uma revisão sistemática literatura científica, baseada em artigos publicados entre 2012 2023, língua portuguesa, inglesa espanhola, disponíveis nas seguintes bases dados: Pubmed, Scielo (Scientific Eletronic Library Online) BVS. Serão incluídos apresentem informações relevantes sobre tema principal deste excluídos não estiverem formato artigo, forem revisões literatura, bibliográficas, fugirem temática proposta ou estejam dentro intervalo tempo pré-definido. Resultados: Nesta bibliográfica, foram analisados 10 originais abordaram fatores risco associados à sífilis congênita mostraram recentes melhoraram significativamente detecção tratamento doenças relacionadas ao HPV, particularmente cânceres. Conclusão: Esses apenas complementam, mas frequentemente superam as limitações dos exames citológicos convencionais, Papanicolau, especialmente populações elevado.

---