Isolation and Characterization of Porcine Epidemic Diarrhea Virus G2c Strains Circulating in China from 2021 to 2024 DOI Creative Commons
Xi Lü,

Chen Chen,

Zixuan Wang

et al.

Veterinary Sciences, Journal Year: 2025, Volume and Issue: 12(5), P. 444 - 444

Published: May 6, 2025

Porcine epidemic diarrhea virus (PEDV) is a major pathogen responsible for viral in pigs, causing particularly high mortality neonatal piglets. In recent years, genetic variations PEDV have resulted alterations both its virulence and antigenicity, leading to reduced efficacy of existing vaccines. this study, diarrheal samples were collected from four commercial pig farms the Hubei, Guangxi, Jiangxi provinces, China, which experienced vaccine failure. RT-qPCR confirmed infection, three strains, 2021-HBMC, 2024-JXYX, 2024-JXNC, successfully isolated. Sequence analysis phylogenetic tree construction classified these strains into G2c genotype, predominant subtype China. The neutralization assays revealed significant reduction neutralizing titers against immune serum compared with AJ1102 reference strain. Further amino acid sequence spike (S) protein identified several mutations key epitopes strain, including S27L, E57A, N139D, M214T, P229L S-NTD epitope; A520S, F539L, K566N, D569E, G612V, P634S, E636V/K COE Y1376H 2C10 epitope, along deletions at N-glycosylation sites (347NSSD 510NITV). Additionally, whole-genome sequencing recombination indicated that 2021-HBMC strain may event. findings study underscore challenge posed by continuous evolution provide valuable insights future development control strategies.

Language: Английский

Isolation and Characterization of Porcine Epidemic Diarrhea Virus G2c Strains Circulating in China from 2021 to 2024 DOI Creative Commons
Xi Lü,

Chen Chen,

Zixuan Wang

et al.

Veterinary Sciences, Journal Year: 2025, Volume and Issue: 12(5), P. 444 - 444

Published: May 6, 2025

Porcine epidemic diarrhea virus (PEDV) is a major pathogen responsible for viral in pigs, causing particularly high mortality neonatal piglets. In recent years, genetic variations PEDV have resulted alterations both its virulence and antigenicity, leading to reduced efficacy of existing vaccines. this study, diarrheal samples were collected from four commercial pig farms the Hubei, Guangxi, Jiangxi provinces, China, which experienced vaccine failure. RT-qPCR confirmed infection, three strains, 2021-HBMC, 2024-JXYX, 2024-JXNC, successfully isolated. Sequence analysis phylogenetic tree construction classified these strains into G2c genotype, predominant subtype China. The neutralization assays revealed significant reduction neutralizing titers against immune serum compared with AJ1102 reference strain. Further amino acid sequence spike (S) protein identified several mutations key epitopes strain, including S27L, E57A, N139D, M214T, P229L S-NTD epitope; A520S, F539L, K566N, D569E, G612V, P634S, E636V/K COE Y1376H 2C10 epitope, along deletions at N-glycosylation sites (347NSSD 510NITV). Additionally, whole-genome sequencing recombination indicated that 2021-HBMC strain may event. findings study underscore challenge posed by continuous evolution provide valuable insights future development control strategies.

Language: Английский

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