Circular RNA circSLC26A4 Accelerates Cervical Cancer Progression via miR-1287-5p/HOXA7 Axis

Molecular Therapy — Nucleic Acids, Journal Year: 2019, Volume and Issue: 19, P. 413 - 420

Published: Dec. 6, 2019

Circular RNAs (circRNAs) are group of noncoding derived from back-splicing events. Accumulating evidence certifies the critical roles circRNAs in human tumorigenesis. However, role and biogenesis cervical cancer still unclear. Here, a novel identified circRNA, circSLC26A4, was found to be upregulated tissue cells. Clinically, high expression circSLC26A4 related poor survival patients. Functionally, cellular experiments indicated that knockdown repressed proliferation, invasion, tumor growth vitro vivo. Furthermore, acted as sponge miR-1287-5p; moreover, miR-1287-5p targeted 3′ UTR HOXA7 mRNA. Mechanistically, RNA binding protein (RBP) quaking (QKI) interact with QKI response elements (QREs) SLC26A4 gene introns, thereby promoting biogenesis. In conclusion, these findings demonstrate facilitates progression through QKI/circSLC26A4/miR-1287-5p/HOXA7 axis, which might bring therapeutic strategies for cancer.

Language: Английский

---

The U2AF2 /circRNA ARF1/miR-342–3p/ISL2 feedback loop regulates angiogenesis in glioma stem cells

Journal of Experimental & Clinical Cancer Research, Journal Year: 2020, Volume and Issue: 39(1)

Published: Sept. 7, 2020

Abstract Background Glioma is the most common and lethal primary brain tumor in adults, angiogenesis one of key factors contributing to its proliferation, aggressiveness, malignant transformation. However, discovery novel oncogenes study molecular regulating mechanism based on circular RNAs (circRNAs) may provide a promising treatment target glioma. Methods Bioinformatics analysis, qPCR, western blotting, immunohistochemistry were used detect expression levels ISL2 , miR-342–3p, circRNA ARF1 (cARF1), U2AF2 VEGFA . Patient-derived glioma stem cells (GSCs) established for experiments. Lentiviral-based infection was regulate these molecules GSCs. The MTS, EDU, Transwell, tube formation assays invasion, human microvessel endothelial (hBMECs). RNA-binding protein immunoprecipitation, RNA pull-down, dual-luciferase reporter, chromatin immunoprecipitation direct regulation mechanisms among molecules. Results We first identified transcription factor related neural development. overexpressed correlated with poor patient survival. transcriptionally regulated GSCs promoted hBMECs via -mediated ERK signaling. Regarding action, cARF1 upregulated miR-342–3p sponging. Furthermore, bound stability cARF1, while induced which formed feedback loop also showed that both had an oncogenic effect, glioma, Conclusions Our This angiogenesis, could effective biomarker diagnosis prognostic evaluation, as well possibly being targeted therapy.

Language: Английский

---

Circular RNAs are a novel type of non-coding RNAs in ROS regulation, cardiovascular metabolic inflammations and cancers

Pharmacology & Therapeutics, Journal Year: 2020, Volume and Issue: 220, P. 107715 - 107715

Published: Oct. 24, 2020

Language: Английский

---

Mammalian circular RNAs result largely from splicing errors

Cell Reports, Journal Year: 2021, Volume and Issue: 36(4), P. 109439 - 109439

Published: July 1, 2021

Highlights•The rate of back-splicing for a gene declines with its degree splicing•The abundance in species effective population size•Mammalian circRNAs are overall evolutionarily non-conserved•More than 97% the observed circRNA production is deleteriousSummaryUbiquitous eukaryotes, circular RNAs (circRNAs) comprise large class mostly non-coding produced by back-splicing. Although some have demonstrated biochemical activities, whether most functional unknown. Here, we test hypothesis that primarily results from splicing error and so deleterious instead beneficial. In support hypothesis, our analysis RNA sequencing data 11 shared tissues humans, macaques, mice finds (1) much rarer linear-splicing, (2) diminishes amount, (3) prevalence size, (4) unconserved. We estimate more deleterious. identify small number candidates, genome-wide trend strongly suggests largely non-functional products errors.Graphical abstract

Language: Английский

---

microRNA-Based Biomarkers in Alzheimer’s Disease (AD)

Frontiers in Neuroscience, Journal Year: 2020, Volume and Issue: 14

Published: Oct. 30, 2020

Alzheimer's disease (AD) is a multifactorial, age-related neurological characterized by complex pathophysiological dynamics taking place at multiple biological levels, including molecular, genetic, epigenetic, cellular and large-scale brain networks. These alterations account for mechanisms such as protein accumulation, neuroinflammatory/neuro-immune processes, synaptic dysfunction, neurodegeneration that eventually lead to cognitive behavioral decline. Alterations in microRNA (miRNA) signaling have been implicated the epigenetics molecular genetics of all neurobiological processes associated with AD pathophysiology. changes encompass altered miRNA abundance, speciation complexity anatomical regions CNS targeted disease, modified expression patterns tissues, systemic circulation, extracellular fluid (ECF) cerebrospinal (CSF). miRNAs investigated candidate biomarkers diagnosis, prediction, prognosis therapeutic purposes because their involvement pathways both health disease. In this review we will: (i) highlight significantly heterogeneous nature tissues biofluids; (ii) address how information may be extracted from these data used diagnostic, prognostic and/or screening tools across entire continuum AD, preclinical stage, through prodromal, i.e., mild impairment (MCI) phase way clinically overt dementia; (iii) consider specific could categorized using reporters span initiation progression.

Language: Английский

---