Transcriptional Regulation of Neuropeptide Receptors Decodes Complexity of Peptidergic Modulation of Behavior and Physiology. DOI Creative Commons

SeungHeui Ryu,

Yanan Wei, Zekun Wu

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2024, Volume and Issue: unknown

Published: Nov. 23, 2024

ABSTRACT The modulation of complex behaviors in response to environmental and physiological contexts is a fundamental aspect animal biology, with neuropeptides (NPs) playing crucial role this process. This study investigates the transcriptional regulation neuropeptide receptors (NPRs) as mechanism for context-dependent neuropeptidergic physiology behavior. We hypothesize that control NPR genes, rather than NPs themselves, critical determinant behavior physiology. Using multi-faceted approach, including comparative genomics, transcription factor network analysis, empirical validation model organisms such Drosophila melanogaster , we reveal regulatory landscape where expression tightly controlled. Our findings demonstrate genes exhibit higher number enhancers, CTCF-binding sites, open chromatin regions compared NP suggesting greater susceptibility modulation. architecture allows precise over signaling, enabling dynamic context-specific behavioral responses. results highlight importance NPR-expressing cells by mediating effects on show conserved across species, indicating an evolutionarily significant fine-tuning signaling. Furthermore, our provides insights into distinct mechanisms underlying multifunctionality their receptors, offering novel perspective behaviors. In conclusion, advances understanding signaling focusing NPRs. have broad implications development therapeutic strategies targeting systems various neurological disorders.

Language: Английский

The diverse roles of insulin signaling in insect behavior DOI Creative Commons

Anastasia A. Weger,

Clare C. Rittschof

Frontiers in Insect Science, Journal Year: 2024, Volume and Issue: 4

Published: April 4, 2024

In insects and other animals, nutrition-mediated behaviors are modulated by communication between the brain peripheral systems, a process that relies heavily on insulin/insulin-like growth factor signaling pathway (IIS). Previous studies have focused mechanistic physiological functions of insulin-like peptides (ILPs) in critical developmental adult milestones like pupation or vitellogenesis. Less work has detailed mechanisms connecting ILPs to nutrient-mediated related survival reproductive success. Here we briefly review range linked IIS insects, from conserved regulation feeding behavior evolutionarily derived polyphenisms. Where possible, incorporate information Drosophila melanogaster model species describe molecular neural connect nutritional status behavioral expression via IIS. We identify knowledge gaps which include diverse functional roles ILPs, how modulate function across lifespan, lack research broad taxa. Addressing these would enable better understanding evolution this widely deployed tool kit pathway.

Language: Английский

Citations

13
Precise measurement of motor neuron dysfunction in Drosophila ALS model via climbing assay and leg imaging DOI

Yanan Wei,

Hongyu Miao, Hadi Najafi

et al.

Methods in cell biology, Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 1, 2025

Language: Английский

Citations

0
Surface glia for modeling ALS-FTD-associated mutant C9orf72 toxicity in the nervous system of Drosophila DOI Creative Commons

Yanan Wei,

Brittany Anne Snow,

Ciara Crowley Stevenson

et al.

Genes & Diseases, Journal Year: 2025, Volume and Issue: unknown, P. 101629 - 101629

Published: April 1, 2025

Language: Английский

Citations

0
The astrocyte-enriched gene deathstar plays a crucial role in the development, locomotion, and lifespan of D. melanogaster DOI Creative Commons
Xiaoli Zhang,

Dongyu Sun,

Kyle Wong

et al.

Fly, Journal Year: 2024, Volume and Issue: 18(1)

Published: June 17, 2024

The Drosophila melanogaster brain is a complex organ with various cell types, orchestrating the development, physiology, and behaviors of fly. While each type in known to express unique gene set, their complete genetic profile still unknown. Advances RNA sequencing techniques at single-cell resolution facilitate identifying novel markers and/or re-examining specificity available ones. In this study, exploiting data optic lobe, we categorized cells based on expression pattern for markers, then genes enriched astrocytes were identified. CG11000 was identified as comparable Eaat1 gene, an astrocyte marker, every individual inside lobe midbrain, well entire throughout its development. Consistent our bioinformatics data, immunostaining brains dissected from transgenic adult flies showed co-expression set single corresponding brain. Physiologically, inhibiting through interference disrupted normal development male D. melanogaster, while having no impact females. Expression suppression led decreased locomotion activity also shortened lifespan specifically astrocytes, indicating gene's significance astrocytes. We designated 'deathstar' due crucial role maintaining star-like shape glial cells, into stage.

Language: Английский

Citations

3
Hypersensitivity controlled by mir-9a modulates female receptivity ofDrosophila melanogaster DOI Open Access
Xiaoli Zhang, Joshua A. Bagley, Huang Yongwen

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2024, Volume and Issue: unknown

Published: July 29, 2024

ABSTRACT Female Drosophila melanogaster undergo a complex behavioral transformation following mating, characterized by increased sensory sensitivity and altered reproductive behaviors. In this study, we investigated the role of miR-9a, conserved microRNA, in regulating these post-mating changes. We found that miR-9a mutant females exhibited hypersensitivity phenotype, with rejection courting males, delayed onset sexual receptivity, abnormal mating termination behavior. This phenotype was associated aberrant overgrowth adult body wall neurons, suggesting link between neuronal To further elucidate underlying mechanisms, performed genetic interaction studies sens bru2 , genes known to interact miR-9a. removing one copy or backgrounds rescued female normalized morphology. suggests regulates neuron development receptivity modulating expression target like . Our findings reveal novel for behaviors research provides valuable insights into molecular mechanisms adaptations neural development. Additionally, our highlight potential as model organism investigating miR-9 family members specification function, implications understanding processing plasticity other organisms.

Language: Английский

Citations

0
Transcriptional Regulation of Neuropeptide Receptors Decodes Complexity of Peptidergic Modulation of Behavior and Physiology. DOI Creative Commons

SeungHeui Ryu,

Yanan Wei, Zekun Wu

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2024, Volume and Issue: unknown

Published: Nov. 23, 2024

ABSTRACT The modulation of complex behaviors in response to environmental and physiological contexts is a fundamental aspect animal biology, with neuropeptides (NPs) playing crucial role this process. This study investigates the transcriptional regulation neuropeptide receptors (NPRs) as mechanism for context-dependent neuropeptidergic physiology behavior. We hypothesize that control NPR genes, rather than NPs themselves, critical determinant behavior physiology. Using multi-faceted approach, including comparative genomics, transcription factor network analysis, empirical validation model organisms such Drosophila melanogaster , we reveal regulatory landscape where expression tightly controlled. Our findings demonstrate genes exhibit higher number enhancers, CTCF-binding sites, open chromatin regions compared NP suggesting greater susceptibility modulation. architecture allows precise over signaling, enabling dynamic context-specific behavioral responses. results highlight importance NPR-expressing cells by mediating effects on show conserved across species, indicating an evolutionarily significant fine-tuning signaling. Furthermore, our provides insights into distinct mechanisms underlying multifunctionality their receptors, offering novel perspective behaviors. In conclusion, advances understanding signaling focusing NPRs. have broad implications development therapeutic strategies targeting systems various neurological disorders.

Language: Английский

Citations

0