The effects of acteoside on locomotor recovery after spinal cord injury – The role of autophagy and apoptosis signaling pathway

Biomedicine & Pharmacotherapy, Journal Year: 2024, Volume and Issue: 175, P. 116607 - 116607

Published: April 30, 2024

In the current study, we investigated effects of acteoside as a phenylpropanoid glycoside on interaction with neurons to assesses locomotor recovery after spinal cord injury (SCI) in rats by focusing evaluating factors involved autophagy, apoptosis, inflammation and oxidative stress processes. 49 Spargue-Dawley were prepared divided into seven healthy SCI groups receiving different concentrations acteoside. After 28 days disease induction treatment acteoside, BBB score test was used evaluate activity. Then, preparing cell homogenates, expression levels MAP1LC3A, MAP-2, glial fibrillary acidic protein (GFAP), Nrf2, Keap-1, Caspase 3 (Casp3), Bax, Bcl-2, TNF-a, IL-1B, reactive oxygen species (ROS), malondialdehyde (MDA) measured. Improvement activity observed two weeks beginning experiment continued until fourth week. Both MAP1LC3A MAP-2 significantly up-regulated treated compared untreated rats, GFAP decreased these animals. Pro-apoptotic proteins Bax Casp3 anti-apoptotic Bcl-2 down-regulated up-regulated, respectively, addition, significant downregulation iNOS, TNF-α, IL-1β decrease contents both ROS MDA well increases Nrf2 Keap-1 seen Furthermore, strongly interacted targets binding affinities -8.3 kcal/mol, -8.5 determined molecular docking studies. general, it can be concluded that has protective considered an adjuvant therapy this disease. However, more studies, especially clinical are needed field.

Language: Английский

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High Dose C6 Ceramide-Induced Response in Embryonic Hippocampal Cells

Biomolecules, Journal Year: 2025, Volume and Issue: 15(3), P. 430 - 430

Published: March 17, 2025

Ceramide is a critical molecule in both the physiology and pathology of central nervous system. The most studied aspect its effect on embryonic/stem cells. A salient question whether low doses ceramide induce neuronal differentiation without interfering with sphingolipid metabolism high can be used glioblastoma for their cytotoxic effect. Here, we examined dose (13 µM) HN9.10e Interestingly, 13 µM induced an immediate increase cell viability, followed by number mitochondria. Microscopic morphometric analysis revealed decrease differentiated cells compared to 0.1 but longer neurites. Furthermore, lipidomic study demonstrated formation medium–long-chain sphingomyelin species sphingosine 1 phosphate. Sphingolipid modification correlated SMPD3, ASAH2, SPHK2 gene expression coding neutral sphingomyenase 2, ceramidase kinase respectively. Overall, our data show that variety responses same type dependent concentration used. Low do not affect metabolism, so different cellular response.

Language: Английский

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The combined damage of bisphenol A and high fat diet to learning and memory in young male mice: the regulatory effect of BDNF/TrkB/PI3K/AKT pathway on autophagy

Environmental Research, Journal Year: 2025, Volume and Issue: unknown, P. 121538 - 121538

Published: April 1, 2025

Language: Английский

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Present and future use of exosomes containing proteins and RNAs in neurodegenerative diseases for synaptic function regulation: A comprehensive review

International Journal of Biological Macromolecules, Journal Year: 2024, Volume and Issue: 280, P. 135826 - 135826

Published: Sept. 23, 2024

Neurodegenerative diseases (NDDs) are increasingly prevalent with global aging, demanding effective treatments. Exosomes, which contain biological macromolecules such as RNA (including miRNAs) and proteins like α-synuclein, tau, amyloid-beta, gaining attention innovative therapeutics. This comprehensive review systematically explores the potential roles of exosomes in NDDs, a particular focus on their role synaptic dysfunction. We present pathophysiology NDDs discuss mechanisms exosome formation, secretion, action. Subsequently, we different types Alzheimer's disease Parkinson's disease, special regulation function. In addition, explore use biomarkers, well challenges opportunities clinical application. provide perspectives future research directions development trends to more understanding guidance for application treatment NDDs. conclusion, rich macromolecules, novel therapeutic strategy, have opened up new possibilities brought hope patients.

Language: Английский

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Tribotronics for bioinspired neuromorphic tactile perception and computing

MRS Bulletin, Journal Year: 2024, Volume and Issue: unknown

Published: Dec. 31, 2024

Language: Английский

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