Synergistic effects of Trichoderma and biochar on the biocontrol of two soil-borne phytopathogens in chickpeas DOI Creative Commons
Ranjna Kumari, Vipul Kumar, Bhupendra Koul

et al.

Frontiers in Microbiology, Journal Year: 2025, Volume and Issue: 16

Published: May 1, 2025

Introduction This study aims to identify and characterize four Trichoderma isolates using molecular techniques, Fourier transform infrared spectroscopy (FTIR), volatile organic compounds (VOC) profiling. Methods The antagonistic activity of these was assessed against Fusarium oxysporum f. sp. ciceri (FOC) Sclerotium rolfsii (SR) a dual culture technique. synergistic effect harzianum (accession no. PP256488) combined with biochar (BC) evaluated for plant growth enhancement disease suppression. Four ( T. harzianum, asperellum, virens , lixii ) were identified through ITS region analysis, VOC profiling, FTIR spectroscopy. Results Molecular analysis confirmed their distinct identities, GC-MS revealed 37 VOCs out 162 antipathogenic properties. Unique peaks recorded at 3271.96 cm −1 2800–2900 asperellum 2850–2950 both . Scanning electron microscopy (SEM) mycoparasitic structures, including hyphal coils, penetration holes, appressoria, indicating effective pathogen interaction. application (T9) significantly enhanced root length (9.23 cm), height (26.03 mass (43.33 g) in chickpea plants. Moreover, treatments (T10) reduced the incidence chickpeas, decreasing fusarium wilt by 27% collar rot 33%, respectively. Conclusion sustainable approach exhibits potential which can enhance reduce incidence, improve food security.

Language: Английский

Synergistic effects of Trichoderma and biochar on the biocontrol of two soil-borne phytopathogens in chickpeas DOI Creative Commons
Ranjna Kumari, Vipul Kumar, Bhupendra Koul

et al.

Frontiers in Microbiology, Journal Year: 2025, Volume and Issue: 16

Published: May 1, 2025

Introduction This study aims to identify and characterize four Trichoderma isolates using molecular techniques, Fourier transform infrared spectroscopy (FTIR), volatile organic compounds (VOC) profiling. Methods The antagonistic activity of these was assessed against Fusarium oxysporum f. sp. ciceri (FOC) Sclerotium rolfsii (SR) a dual culture technique. synergistic effect harzianum (accession no. PP256488) combined with biochar (BC) evaluated for plant growth enhancement disease suppression. Four ( T. harzianum, asperellum, virens , lixii ) were identified through ITS region analysis, VOC profiling, FTIR spectroscopy. Results Molecular analysis confirmed their distinct identities, GC-MS revealed 37 VOCs out 162 antipathogenic properties. Unique peaks recorded at 3271.96 cm −1 2800–2900 asperellum 2850–2950 both . Scanning electron microscopy (SEM) mycoparasitic structures, including hyphal coils, penetration holes, appressoria, indicating effective pathogen interaction. application (T9) significantly enhanced root length (9.23 cm), height (26.03 mass (43.33 g) in chickpea plants. Moreover, treatments (T10) reduced the incidence chickpeas, decreasing fusarium wilt by 27% collar rot 33%, respectively. Conclusion sustainable approach exhibits potential which can enhance reduce incidence, improve food security.

Language: Английский

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