Analysis of the value of potential biomarker S100‐A8 protein in the diagnosis and pathogenesis of spinal tuberculosis DOI Creative Commons
Zhibo Ren,

Jinke Ji,

Caili Lou

et al.

JOR Spine, Journal Year: 2024, Volume and Issue: 7(2)

Published: April 10, 2024

Abstract Objectives The objective of this study is to evaluate the value S100‐A8 protein as a diagnostic marker for spinal tuberculosis and explore its role in potential pathogenesis (STB). Methods peripheral blood 100 patients admitted General Hospital Ningxia Medical University from September 2018 June 2021 were collected observation group, 30 healthy medical examiners control group. Three samples group three selected proteomics detection screening differential proteins. Kyoto Encyclopedia Genes (KEGG) was used enrich analyze related signaling pathways confirm target protein. serum expression levels proteins determined compared between two groups using enzyme‐linked immunosorbent assay (ELISA). Statistical methods STB. A macrophage model Mycobacterium infection constructed small interfering RNA investigate molecular mechanism Results has diagnosing (AUC = 0.931, p < 0.001), level (59.04 ± 19.37 ng/mL) significantly higher than that (43.16 10.07 ( 0.05). showed significant positive correlation with both CRP ESR values 0.01). Its AUCs combined bacteriological detection, T‐SPOT results, imaging, antacid staining pathological results 0.705 0.05), 0.754 0.01), 0.716 0.656 0.681 respectively. Lack leads decrease TLR4 IL‐17A infected macrophages. Conclusion differentially expressed individuals may be novel candidate biomarker diagnosis tuberculosis. feedback loop on S100‐A8‐TLR4‐IL‐17A axis play an important inflammatory

Language: Английский

Analysis of the value of potential biomarker S100‐A8 protein in the diagnosis and pathogenesis of spinal tuberculosis DOI Creative Commons
Zhibo Ren,

Jinke Ji,

Caili Lou

et al.

JOR Spine, Journal Year: 2024, Volume and Issue: 7(2)

Published: April 10, 2024

Abstract Objectives The objective of this study is to evaluate the value S100‐A8 protein as a diagnostic marker for spinal tuberculosis and explore its role in potential pathogenesis (STB). Methods peripheral blood 100 patients admitted General Hospital Ningxia Medical University from September 2018 June 2021 were collected observation group, 30 healthy medical examiners control group. Three samples group three selected proteomics detection screening differential proteins. Kyoto Encyclopedia Genes (KEGG) was used enrich analyze related signaling pathways confirm target protein. serum expression levels proteins determined compared between two groups using enzyme‐linked immunosorbent assay (ELISA). Statistical methods STB. A macrophage model Mycobacterium infection constructed small interfering RNA investigate molecular mechanism Results has diagnosing (AUC = 0.931, p < 0.001), level (59.04 ± 19.37 ng/mL) significantly higher than that (43.16 10.07 ( 0.05). showed significant positive correlation with both CRP ESR values 0.01). Its AUCs combined bacteriological detection, T‐SPOT results, imaging, antacid staining pathological results 0.705 0.05), 0.754 0.01), 0.716 0.656 0.681 respectively. Lack leads decrease TLR4 IL‐17A infected macrophages. Conclusion differentially expressed individuals may be novel candidate biomarker diagnosis tuberculosis. feedback loop on S100‐A8‐TLR4‐IL‐17A axis play an important inflammatory

Language: Английский

Citations

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