Purification, Source Exploration, and Bioactivity Evaluation of Oligosaccharides in Sijunzi Decoction DOI

Xiaonan Chen,

Zong‐Jin Pu,

Yixin Liu

et al.

Journal of Separation Science, Journal Year: 2024, Volume and Issue: 47(19)

Published: Sept. 28, 2024

ABSTRACT Sijunzi decoction (SJZD) has been widely used to treat splenic deficiency syndrome. Previous studies confirmed that polysaccharides and non‐polysaccharides (NPS) are the main active components of SJZD. This study aimed investigate composition activity oligosaccharides in NPS. In this study, oligosaccharide component (named SJZD‐OGS), consisting several different oligosaccharides, was separated purified from SJZD (SJZD_NPS). Ultra‐high‐performance liquid chromatography–ion mobility spectrometry–quadrupole time‐of‐flight mass spectrometry (UHPLC–IMS–QTOF/MS), matrix‐assisted laser desorption/ionization (MALDI‐TOF‐MS), high‐performance anion‐exchange chromatography with pulsed amperometric detection (HPAEC‐PAD) were analyze SJZD‐OGS. SJZD‐OGS, a molecular weight 2178 Da, composed glucose fructose, its chemical mainly included fructo‐oligosaccharides (FOS) degree polymerization 3–14 (DP 3–14). The results source exploration demonstrated SJZD‐OGS each herb addition, showed good anti‐inflammatory on macrophage cells intestinal epithelial cell protective activity. present work provides experimental evidence for elucidating pharmaceutical presents an effective strategy research traditional Chinese medicine (TCM).

Language: Английский

Total saponins from Siraitiae Fructus alleviate lung and intestinal injuries in rats with acute lung injury and mice infected with the influenza virus by regulating cytokines and enhancing the mucosal barrier. DOI

Zong‐Jin Pu,

Yuhang Ma, Xiaonan Chen

et al.

Food Bioscience, Journal Year: 2025, Volume and Issue: unknown, P. 106538 - 106538

Published: April 1, 2025

Language: Английский

Citations

0
Purification, Source Exploration, and Bioactivity Evaluation of Oligosaccharides in Sijunzi Decoction DOI

Xiaonan Chen,

Zong‐Jin Pu,

Yixin Liu

et al.

Journal of Separation Science, Journal Year: 2024, Volume and Issue: 47(19)

Published: Sept. 28, 2024

ABSTRACT Sijunzi decoction (SJZD) has been widely used to treat splenic deficiency syndrome. Previous studies confirmed that polysaccharides and non‐polysaccharides (NPS) are the main active components of SJZD. This study aimed investigate composition activity oligosaccharides in NPS. In this study, oligosaccharide component (named SJZD‐OGS), consisting several different oligosaccharides, was separated purified from SJZD (SJZD_NPS). Ultra‐high‐performance liquid chromatography–ion mobility spectrometry–quadrupole time‐of‐flight mass spectrometry (UHPLC–IMS–QTOF/MS), matrix‐assisted laser desorption/ionization (MALDI‐TOF‐MS), high‐performance anion‐exchange chromatography with pulsed amperometric detection (HPAEC‐PAD) were analyze SJZD‐OGS. SJZD‐OGS, a molecular weight 2178 Da, composed glucose fructose, its chemical mainly included fructo‐oligosaccharides (FOS) degree polymerization 3–14 (DP 3–14). The results source exploration demonstrated SJZD‐OGS each herb addition, showed good anti‐inflammatory on macrophage cells intestinal epithelial cell protective activity. present work provides experimental evidence for elucidating pharmaceutical presents an effective strategy research traditional Chinese medicine (TCM).

Language: Английский

Citations

0