A versatile and automatic on-line screening method: Transverse diffusion of laminar flow profiles-based capillary electrophoresis for exploring PTP1B inhibitors in natural products DOI
Juan Gao, Erwin Adams, Zhengjin Jiang

et al.

Journal of Chromatography A, Journal Year: 2024, Volume and Issue: 1741, P. 465607 - 465607

Published: Dec. 18, 2024

Language: Английский

Recent advances of capillary electrophoresis for enzyme analysis (2021–2024) DOI

Y. Y. Thomas Su,

範夫 清水,

Jia Tang

et al.

Microchemical Journal, Journal Year: 2025, Volume and Issue: unknown, P. 113701 - 113701

Published: April 1, 2025

Language: Английский

Citations

0
Determination of Nine Organophosphorus Pesticide Residues in Corn by Capillary Electrophoresis with Solid Phase Extraction DOI Creative Commons

Chunxiu Gu,

Baining Liu,

Kaowen Zhou

et al.

International Journal of Electrochemical Science, Journal Year: 2024, Volume and Issue: unknown, P. 100845 - 100845

Published: Oct. 1, 2024

Language: Английский

Citations

2
Screening thrombin inhibitors from Yangxinshi tablets by online capillary electrophoresis‐based immobilized enzyme microreactor and molecular docking DOI
Wenping Liu, Rui Zhou,

Jiake Wen

et al.

Phytochemical Analysis, Journal Year: 2024, Volume and Issue: unknown

Published: Sept. 15, 2024

Abstract Introduction Yangxinshi tablet (YXST) is a effective traditional Chinese medicine in treating cardiovascular diseases such as heart failure and myocardial infarction. Objectives This study aims to develop method for screening thrombin inhibitors from YXST using an online immobilized enzyme microreactor (IMER) based on capillary electrophoresis (CE). Materials methods Thrombin (THR) was the capillary's inner wall polydopamine (PDA). The chromogenic substrate S‐2238 employed assess activity kinetic parameters. stability repeatability of constructed thrombin‐immobilized (THR‐IMER) were evaluated over 40 runs, maintaining 85% initial activity. Michaelis–Menten constant ( K m ) THR determined be 11.98 mM. half‐maximal inhibitory concentration (IC 50 inhibition i argatroban calculated. Ten compounds screened potency THR‐IMER. Results Salvianolic acid B caffeic identified potential YXST, with rates at 200 μg/mL 55.06 ± 6.70% 31.88 4.79%, respectively, aligning microplate reader assay results. Molecular docking analysis confirmed their interactions key residues, verifying Conclusion CE‐based THR‐IMER successfully developed offering reliable approach identifying therapeutic compounds.

Language: Английский

Citations

0
Multi-enzyme simulation activity of MXene-based metal composites and the application for detection and degradation of phenolic pollutants DOI
He Huang, Qinqin Yang, Yu He

et al.

Microchemical Journal, Journal Year: 2024, Volume and Issue: unknown, P. 112350 - 112350

Published: Dec. 1, 2024

Language: Английский

Citations

0
A versatile and automatic on-line screening method: Transverse diffusion of laminar flow profiles-based capillary electrophoresis for exploring PTP1B inhibitors in natural products DOI
Juan Gao, Erwin Adams, Zhengjin Jiang

et al.

Journal of Chromatography A, Journal Year: 2024, Volume and Issue: 1741, P. 465607 - 465607

Published: Dec. 18, 2024

Language: Английский

Citations

0