Integrated bioinformatics analysis and biological experiments to identify key immune genes in vascular dementia DOI Creative Commons
Yilong Zhao, Wen Xing, Weiqi Chen

et al.

Frontiers in Immunology, Journal Year: 2025, Volume and Issue: 16

Published: March 24, 2025

Objectives This study aimed to identify key immune genes provide new perspectives on the mechanisms and diagnosis of vascular dementia (VaD) based bioinformatic methods combined with biological experiments in mice. Methods We obtained gene expression profiles from a Gene Expression Omnibus database (GSE186798). The data were analysed using integrated bioinformatics machine learning techniques pinpoint potential immune-related for diagnosing VaD. Moreover, diagnostic accuracy was evaluated through receiver operating characteristic curve analysis. microRNA, transcription factor (TF), drug-regulating hub predicted database. Immune cell infiltration has been studied investigate dysregulation cells patients To evaluate cognitive impairment, mice bilateral common carotid artery stenosis (BCAS) subjected behavioural tests 30 d after chronic cerebral hypoperfusion. BCAS determined quantitative polymerase chain reaction(qPCR). Results results set enrichment variation analyses indicated that pathways upregulated A total 1620 included dataset, 323 differentially expressed examined GSE186798 dataset. Thirteen identified differential Protein-protein interaction network design functional analysis performed system as main subject. value, two core selected learning. Two putative genes, Rac family small GTPase 1( RAC1 ) CKLF-like MARVEL transmembrane domain containing 5 ( CMTM5 exhibit good value. Their high confidence levels confirmed by validating each biomarker different According GeneMANIA, VaD pathophysiology is strongly associated inflammatory responses. used construct miRNA gene, TFs-hub drug-hub networks. Varying also observed. In animal experiments, mouse model employed mimic humans, further Morris water maze test. mRNA significantly reduced group, which consistent Conclusions are frontal lobes mice, suggesting their biomarkers prognosis These findings pave way exploring novel molecular at preventing or treating

Language: Английский

Integrated bioinformatics analysis and biological experiments to identify key immune genes in vascular dementia DOI Creative Commons
Yilong Zhao, Wen Xing, Weiqi Chen

et al.

Frontiers in Immunology, Journal Year: 2025, Volume and Issue: 16

Published: March 24, 2025

Objectives This study aimed to identify key immune genes provide new perspectives on the mechanisms and diagnosis of vascular dementia (VaD) based bioinformatic methods combined with biological experiments in mice. Methods We obtained gene expression profiles from a Gene Expression Omnibus database (GSE186798). The data were analysed using integrated bioinformatics machine learning techniques pinpoint potential immune-related for diagnosing VaD. Moreover, diagnostic accuracy was evaluated through receiver operating characteristic curve analysis. microRNA, transcription factor (TF), drug-regulating hub predicted database. Immune cell infiltration has been studied investigate dysregulation cells patients To evaluate cognitive impairment, mice bilateral common carotid artery stenosis (BCAS) subjected behavioural tests 30 d after chronic cerebral hypoperfusion. BCAS determined quantitative polymerase chain reaction(qPCR). Results results set enrichment variation analyses indicated that pathways upregulated A total 1620 included dataset, 323 differentially expressed examined GSE186798 dataset. Thirteen identified differential Protein-protein interaction network design functional analysis performed system as main subject. value, two core selected learning. Two putative genes, Rac family small GTPase 1( RAC1 ) CKLF-like MARVEL transmembrane domain containing 5 ( CMTM5 exhibit good value. Their high confidence levels confirmed by validating each biomarker different According GeneMANIA, VaD pathophysiology is strongly associated inflammatory responses. used construct miRNA gene, TFs-hub drug-hub networks. Varying also observed. In animal experiments, mouse model employed mimic humans, further Morris water maze test. mRNA significantly reduced group, which consistent Conclusions are frontal lobes mice, suggesting their biomarkers prognosis These findings pave way exploring novel molecular at preventing or treating

Language: Английский

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