Experimental considerations of acute heat stress assays to quantify coral thermal tolerance DOI Creative Commons

JJV Nielsen,

Guillaume Matthews,

KR Frith

et al.

Research Square (Research Square), Journal Year: 2022, Volume and Issue: unknown

Published: May 31, 2022

Abstract Thermal tolerance is variable in corals, yet intrinsic and extrinsic drivers of are not well understood. Understanding the distribution abundance heat tolerant corals across seascapes imperative for predicting responses to climate change support novel management actions. Rapid high-throughput methods measure heat-induced coral bleaching sensitivity increasingly required understand current predict future change. Experimental evaluations typically involve ramp-and-hold experiments run days weeks within aquarium facilities with limits colony replication. Field-based acute stress assays have emerged as an alternative experimental approach rapidly quantify a large number samples role key methodological considerations on response measured remains unresolved. Here, we effects fragment size, sampling time point, physiological measures adult corals. The effect size differed between species ( Acropora tenuis Pocillopora damicornis ). Most parameters here declined over (tissue colour, chlorophyll-a protein content) from onset heating, exception maximum photosynthetic efficiency F v / m ), which was stable up 24h post heating. Based our experiments, identified efficiency, tissue colour change, host-specific such catalase activity rapid quantification thermal tolerance. We recommend that applications include larger fragments (>9cm 2 ) where possible sample 10 - 14h after end stress. A validated combined cost-effective genomic measurements underpins development markers maps ocean warming scenarios.

Language: Английский

Building consensus around the assessment and interpretation of Symbiodiniaceae diversity DOI Creative Commons
Sarah W. Davies, Matthew H. Gamache, Lauren I. Howe‐Kerr

et al.

PeerJ, Journal Year: 2023, Volume and Issue: 11, P. e15023 - e15023

Published: May 2, 2023

Within microeukaryotes, genetic variation and functional sometimes accumulate more quickly than morphological differences. To understand the evolutionary history ecology of such lineages, it is key to examine diversity at multiple levels organization. In dinoflagellate family Symbiodiniaceae, which can form endosymbioses with cnidarians ( e.g ., corals, octocorals, sea anemones, jellyfish), other marine invertebrates e.g. , sponges, molluscs, flatworms), protists foraminifera), molecular data have been used extensively over past three decades describe phenotypes make ecological inferences. Despite advances in Symbiodiniaceae genomics, a lack consensus among researchers respect interpreting has slowed progress field acted as barrier reconciling observations. Here, we identify challenges regarding assessment interpretation across levels: species, populations, communities. We summarize areas agreement highlight techniques approaches that are broadly accepted. where debate remains, unresolved issues discuss technologies help fill knowledge gaps related phenotypic diversity. also ways stimulate progress, particular by fostering inclusive collaborative research community. hope this perspective will inspire accelerate coral reef science serving resource those designing experiments, publishing research, applying for funding their symbiotic partnerships.

Language: Английский

Citations

50
Coral endosymbiont growth is enhanced by metabolic interactions with bacteria DOI Creative Commons
J. L. Matthews, Abeeha Khalil, Nachshon Siboni

et al.

Nature Communications, Journal Year: 2023, Volume and Issue: 14(1)

Published: Oct. 27, 2023

Bacteria are key contributors to microalgae resource acquisition, competitive performance, and functional diversity, but their potential metabolic interactions with coral microalgal endosymbionts (Symbiodiniaceae) have been largely overlooked. Here, we show that altering the bacterial composition of two widespread Symbiodiniaceae species, during free-living stage, results in a significant shift cellular metabolism. Indeed, abundance monosaccharides phytohormone indole-3-acetic acid (IAA) were correlated presence specific bacteria, including members Labrenzia (Roseibium) Marinobacter genera. Single-cell stable isotope tracking revealed these genera involved reciprocal exchanges carbon nitrogen Symbiodiniaceae. We identified provision IAA by Marinobacter, this metabolite caused growth enhancement By unravelling interkingdom interactions, our work demonstrates how associates fundamentally govern fitness.

Language: Английский

Citations

24
The diversity and ecology of Symbiodiniaceae: A traits-based review DOI
Matthew R. Nitschke, Sabrina L. Rosset, Clinton A. Oakley

et al.

Advances in marine biology, Journal Year: 2022, Volume and Issue: unknown, P. 55 - 127

Published: Jan. 1, 2022

Language: Английский

Citations

38
Deoxygenation lowers the thermal threshold of coral bleaching DOI Creative Commons
Rachel Alderdice, Gabriela Perna, Anny Cárdenas

et al.

Scientific Reports, Journal Year: 2022, Volume and Issue: 12(1)

Published: Oct. 31, 2022

Abstract Exposure to deoxygenation from climate warming and pollution is emerging as a contributing factor of coral bleaching mortality. However, the combined effects heating on susceptibility remain unknown. Here, we employed short-term thermal stress assays show that deoxygenated seawater can lower limit an Acropora by much 1 °C or 0.4 based index scores dark-acclimated photosynthetic efficiencies, respectively. Using RNA-Seq, similar responses heat with without seawater, both activating putative key genes hypoxia-inducible response system indicative cellular hypoxia. We also detect distinct responses, including disruption O 2 -dependent photo-reception/-protection, redox status, activation immune prior onset bleaching. Thus, corals are even more vulnerable when faced in waters. This highlights need integrate dissolved measurements into global monitoring programs reefs.

Language: Английский

Citations

35
Contrasting the thermal performance of cultured coral endosymbiont photo-physiology DOI
Nicole J. Dilernia, Emma F. Camp, Natasha Bartels

et al.

Journal of Experimental Marine Biology and Ecology, Journal Year: 2023, Volume and Issue: 561, P. 151865 - 151865

Published: Jan. 4, 2023

Language: Английский

Citations

15
Widespread oxyregulation in tropical corals under hypoxia DOI
David Hughes,

James Edward Alexander,

Gary Cobbs

et al.

Marine Pollution Bulletin, Journal Year: 2022, Volume and Issue: 179, P. 113722 - 113722

Published: May 7, 2022

Language: Английский

Citations

19
A phenomic modeling approach for using chlorophyll-a fluorescence-based measurements on coral photosymbionts DOI Creative Commons
Kenneth D. Hoadley, Grant Lockridge,

Audrey McQuagge

et al.

Frontiers in Marine Science, Journal Year: 2023, Volume and Issue: 10

Published: March 2, 2023

We test a newly developed instrument prototype which utilizes time-resolved chlorophyll- fluorescence techniques and fluctuating light to characterize Symbiodiniaceae functional traits across seven different coral species under cultivation as part of ongoing restoration efforts in the Florida Keys. While traditional only provide handful algal biometrics, system protocol we have generates > 1000 dynamic measurements short (~11 min) time frame. Resulting ‘high-content’ biometric data revealed distinct phenotypes, broadly corresponded genus-level designations determined using quantitative PCR. Next, from Acropora cervicornis (10 genotypes) A. palmata (5 fragments was correlated with bleaching response metrics collected after two month-long exposure high temperature. A network analysis identified 1973 correlations (Spearman R 0.5) between biometrics various metrics. These biomarkers thermal stress were then utilized train predictive model, when tested against same fragments, yielded correlation (R = 0.92) measured (reductions absorbance by chlorophyll-a). When applied all species, model ranked dominated Cladocopium or Breviolum symbionts more susceptible than corals harboring thermally tolerant ( Durusdinium ). direct testing predictions on novel genotypes is still needed, our device modeling pipeline may help broaden scalability existing approaches for determining tolerance reef corals. Our analytical aligns recent assessments that call development tools improving programs.

Language: Английский

Citations

11
Experimental considerations of acute heat stress assays to quantify coral thermal tolerance DOI Creative Commons
J. J. V. Nielsen,

G. Matthews,

K. R. Frith

et al.

Scientific Reports, Journal Year: 2022, Volume and Issue: 12(1)

Published: Oct. 7, 2022

Abstract Understanding the distribution and abundance of heat tolerant corals across seascapes is imperative for predicting responses to climate change support novel management actions. Thermal tolerance variable in intrinsic extrinsic drivers are not well understood. Traditional experimental evaluations coral bleaching typically involve ramp-and-hold experiments run days weeks within aquarium facilities with limits colony replication. Field-based acute stress assays have emerged as an alternative approach rapidly quantify many samples yet role key methodological considerations on response measured remains unresolved. Here, we effects fragment size, sampling time point, physiological measures adult corals. The effect size differed between species ( Acropora tenuis Pocillopora damicornis ). Most parameters here declined over (tissue colour, chlorophyll- a protein content) from onset heating, exception maximum photosynthetic efficiency F v / m ) which was surprisingly stable this scale. Based our experiments, identified efficiency, tissue colour change, host-specific such catalase activity rapid quantification thermal tolerance. We recommend that future applications include larger fragments (> 9 cm 2 where possible sample 10 24 h after end stress. A validated high-throughput combined cost-effective genomic measurements underpins development markers maps ocean warming scenarios.

Language: Английский

Citations

19
Diatom responses to rapid light and temperature fluctuations: adaptive strategies and natural variability DOI Creative Commons

Emma Juffermans,

Lilian Hoch,

Kittikun Songsomboon

et al.

Frontiers in Photobiology, Journal Year: 2025, Volume and Issue: 3

Published: Jan. 27, 2025

Diatoms are crucial in global primary productivity and carbon sequestration, contributing significantly to marine food webs biogeochemical cycles. With the projected increase sea surface temperatures, climate change poses significant threats these essential organisms. This study investigates photobiological responses of nine diatom species rapid changes light temperature, aiming understand their adaptability resilience climate-induced environmental fluctuations. Using a high-throughput phenoplate assay, we evaluated maximum quantum yield photosystem 2 (Fv/Fm), non-photochemical quenching (NPQ) additional photosynthetic parameters under varying temperature conditions. Our results revealed variability photophysiological among species, with emerging as dominant abiotic factor relative light, accounting for 13.2%–37.5% measured variability. Measurements effect size on Fv/Fm showed that there is innate samples when homogeneous culture fractioned 384 subpopulations. Furthermore, hierarchical clustering analysis all identified two distinct groups. One group exhibited strong interaction between intensity suggesting active synergetic mechanisms cope fluctuating environments, while other potential limitations this regard. These findings highlight diatoms’ diverse strategies optimize photosynthesis manage thermal stress, providing insights into future scenarios. demonstrate using method presented work can functionally cluster different species.

Language: Английский

Citations

0
Novel infrastructure for coral gardening and reefscaping DOI Creative Commons
Sebastian Schmidt‐Roach, Rebecca Klaus,

Abdulaziz Al‐Suwailem

et al.

Frontiers in Marine Science, Journal Year: 2023, Volume and Issue: 10

Published: Feb. 10, 2023

Since 1950, coral abundance has declined worldwide by an estimated 60%, and further dramatic declines are predicted. Although global reductions in carbon emissions essential to prevent loss, reef restoration become imperative maintain the ecosystem services that reefs provide humans at local scales. Yet, currently gardening efforts too expensive scale up due labor-intensive nature of methods low success rates. Here, we present a suite technologies improve rehabilitation’s scalability, efficiency, effectiveness. Our modular designed streamline ex situ nursery workflows, reduce maintenance times, solve problems transporting corals outplanting sites, enable rapid on natural artificial substrates. These novel structures can act as seeding hubs, which placed strategically, have capacity enhance reproduction replenish degraded nearby with larvae. They be applied reefscaping, complemented unique eco-friendly, low-carbon-emission for creation architecturally visually appealing habitats underwater landscapes. integrate monitoring approaches support intelligent solutions track genotypes, optimize control stock management, apply assisted evolution approaches, adaptive management through long-term monitoring.

Language: Английский

Citations

7