Bio-optical signatures ofinsituphotosymbionts predict bleaching severity prior to thermal stress in the Caribbean coral speciesAcropora palmata

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2023, Volume and Issue: unknown

Published: July 9, 2023

Abstract The identification of bleaching tolerant traits among individual corals is a major focus for many restoration and conservation initiatives but often relies on large scale or high-throughput experimental manipulations which may not be accessible to front-line practitioners. Here we evaluate machine learning technique generate predictive model estimates severity using non-destructive chlorophyll-a fluorescence photophysiological metrics measured with low-cost open access bio-optical tool. First, four-week long thermal experiment was performed 156 genotypes Acropora palmata at land-based facility. Resulting responses (percent change in Fv/Fm Absorbance) significantly differed across the four distinct phenotypes generated via photophysiology-based dendrogram, indicating strong concordance between fluorescence-based future severity. Next, these correlations were used train then test Random Forest algorithm-based bootstrap resampling technique. Correlation predicted actual significant ( p < 0.0001) increased number training (Peak average R 2 values 0.42 0.33 absorbance, respectively). Strong provide highly scalable means assessing reef corals.

Language: Английский

Regulation of cultured coral endosymbiont photophysiology by alternate heat stress protocols

Marine Biology, Journal Year: 2023, Volume and Issue: 171(1)

Published: Dec. 14, 2023

Language: Английский

Experimental considerations of acute heat stress assays to quantify coral thermal tolerance

Research Square (Research Square), Journal Year: 2022, Volume and Issue: unknown

Published: May 31, 2022

Abstract Thermal tolerance is variable in corals, yet intrinsic and extrinsic drivers of are not well understood. Understanding the distribution abundance heat tolerant corals across seascapes imperative for predicting responses to climate change support novel management actions. Rapid high-throughput methods measure heat-induced coral bleaching sensitivity increasingly required understand current predict future change. Experimental evaluations typically involve ramp-and-hold experiments run days weeks within aquarium facilities with limits colony replication. Field-based acute stress assays have emerged as an alternative experimental approach rapidly quantify a large number samples role key methodological considerations on response measured remains unresolved. Here, we effects fragment size, sampling time point, physiological measures adult corals. The effect size differed between species ( Acropora tenuis Pocillopora damicornis ). Most parameters here declined over (tissue colour, chlorophyll-a protein content) from onset heating, exception maximum photosynthetic efficiency F v / m ), which was stable up 24h post heating. Based our experiments, identified efficiency, tissue colour change, host-specific such catalase activity rapid quantification thermal tolerance. We recommend that applications include larger fragments (>9cm 2 ) where possible sample 10 - 14h after end stress. A validated combined cost-effective genomic measurements underpins development markers maps ocean warming scenarios.

Language: Английский