Infrared and Laser Engineering, Journal Year: 2024, Volume and Issue: 53(9), P. 20240347 - 20240347
Published: Jan. 1, 2024
Biomedical Optics Express, Journal Year: 2025, Volume and Issue: 16(2), P. 837 - 837
Published: Jan. 15, 2025
Optical diffraction tomography (ODT) is an important technique for three-dimensional (3D) imaging of semi-transparent biological samples, enabling volumetric visualization living cells, cultures, and tissues without the need exogenous dyes. However, ODT faces significant challenges in complex specimens due to limited specificity refractive index (RI) coupled relationship between absorption phase image formation. Here, we present multi-modal transport intensity (MM-TIDT), a high-speed 3D microscopy that integrates electrically tunable lens with modified illumination patterns decouple information. Leveraging dual schemes-circular annular apertures-MM-TIDT acquires two stacks, facilitating accurate decoupling. Based on alternating direction method multipliers (ADMM) framework total variation (TV) non-negativity regularization, our reconstructs distributions fluorescence RI high accuracy robustness. Experimental validation fluorescent polystyrene microspheres, Spirulina specimens, DAPI-labeled C166 cells demonstrates capability MM-TIDT resolve fine structural details across diverse sample types, providing versatile platform exploring dynamic processes intricate cellular interactions.
Language: Английский
Citations
1
Laser & Photonics Review, Journal Year: 2025, Volume and Issue: unknown
Published: Feb. 28, 2025
Abstract Intensity diffraction tomography (IDT) and fluorescence dual‐modality imaging facilitate a comprehensive analysis of biological components their interactions. However, long‐term is typically compromised by environmental thermal fluctuations mechanical disturbances from the microscope, leading to time‐varying aberrations focus drift that degrades performance accuracy colocalization. To counter these issues, an adaptive optics‐assisted 3D method (AO‐FIDT) developed. The utilizes innovative iterative ptychographic approach, paired with annular matched illumination conditions, precisely reconstruct IDT results characterize in real‐time. Furthermore, feedback on real‐time point spread function system provided synchronously correct results. efficacy precision AO‐FIDT are confirmed through long‐term, high‐resolution HeLa cells. scrutinizing morphological characteristics subcellular organelles live COS‐7 cells, including progressive sphericity mitochondria under phototoxicity, monitoring continuous changes mitochondrial dynamics throughout cell division process, broad applicability analyzing organelle structure demonstrated.
Language: Английский
Citations
1
Laser & Photonics Review, Journal Year: 2025, Volume and Issue: unknown
Published: May 1, 2025
Abstract Fluorescence microscopy is an indispensable tool for investigating cellular and tissue‐level biology, yet its performance often limited by optical diffraction, aberrations, noise, resulting in suboptimal imaging quality. Traditional adaptive optics (AO) methods typically rely on additional hardware, such as wavefront sensors, to measure correct system which can be both complex costly. Here, a computational technique based sparse blind deconvolution (CAO‐SBD) introduced, uses single blurred image estimate aberrations perform deblurring. By incorporating priors of fluorescent specimens with Zernike polynomial‐based aberration modeling, CAO‐SBD allows the simultaneous reconstruction aberrated point spread function (PSF) sample information, eliminating need precise PSF calibration. This method outperforms traditional Richardson‐Lucy enhancing robustness noise stabilizing process through correction. Experimental results bovine pulmonary artery endothelial cells demonstrate that significantly enhances resolution contrast across wide‐field confocal fluorescence microscopic systems, positioning powerful high‐resolution biological broad applications.
Language: Английский
Citations
0
Frontiers in Physics, Journal Year: 2024, Volume and Issue: 12
Published: Sept. 27, 2024
In this work, we propose a structured-illumination lensless digital holographic microscopy (SI-LDHM). SI-LDHM illuminates sample with 24 structured illuminations (8 orientations × 3 phase shifts) and records the defocused interferogram formed by two copies of object waves along ±1st diffraction orders each SI. The reconstructed under different illumination are respectively propagated to plane +1st order then averaged, thus yielding clean image without artifact twin images. Experimental results demonstrated that thanks multi-oriented SI strategy, in is sevenfold reduced compared conventional DHM, while spatial resolution 1.15 times higher.
Language: Английский
Citations
0
Infrared and Laser Engineering, Journal Year: 2024, Volume and Issue: 53(9), P. 20240347 - 20240347
Published: Jan. 1, 2024
Citations
0