In Situ Formation of Nanoparticles from Graft Copolypeptides Under Dispersion Polymerization Conditions DOI
Ernesto Tinajero‐Díaz, Robert Murphy, Bo Li

et al.

Macromolecular Rapid Communications, Journal Year: 2025, Volume and Issue: unknown

Published: April 28, 2025

Abstract A simple method is presented for preparing polypeptide nanoparticles using hydrophilic biosynthetic ε‐poly(lysine) (εPL) as a reactive surfactant under dispersion polymerization conditions. In situ graft of benzyl‐L‐glutamic acid N ‐carboxyanhydride (BLG‐NCA) triggers the self‐assembly amphiphilic copolymers into nanoparticles, which are colloidally stabilized by remaining εPL amino groups at particle surface. The average nanoparticle diameter can be controlled in range 40–120 nm varying initiator‐to‐NCA ratio, demonstrated correlation between copolymer molecular weight (measured size exclusion chromatography) and z‐average dynamic light scattering). Secondary structure analysis indicates that α‐helical conformation poly(benzyl‐L‐glutamate) (PBLG) grafts plays role both accelerating NCA stabilizing nanostructures. This approach readily scalable to high concentrations offers straightforward route peptidomimetic entirely composed acids, with promising potential nanomedicine applications.

Language: Английский

Polymerization-induced self-assembly of N-substituted glycine N-carboxyanhydrides in selected solvents DOI

Qing Gao,

Hailin Fu, Hui Liu

et al.

European Polymer Journal, Journal Year: 2025, Volume and Issue: 225, P. 113720 - 113720

Published: Jan. 4, 2025

Language: Английский

Citations

1
Poly(l-proline)-Stabilized Polypeptide Nanostructures via Ring-Opening Polymerization-Induced Self-Assembly (ROPISA) DOI Creative Commons
Ernesto Tinajero‐Díaz, Nicola Judge, Bo Li

et al.

ACS Macro Letters, Journal Year: 2024, Volume and Issue: 13(8), P. 1031 - 1036

Published: July 29, 2024

Poly(proline) II helical motifs located at the protein–water interface stabilize three-dimensional structures of natural proteins. Reported here is first example synthetic biomimetic poly(proline)-stabilized polypeptide nanostructures obtained by a straightforward ring-opening polymerization-induced self-assembly (ROPISA) process through consecutive N-carboxyanhydride (NCA) polymerization. It was found that use multifunctional 8-arm initiators critical for formation nanoparticles. Worm-like micelles as well spherical morphologies were confirmed dynamic light scattering (DLS), transmission electron microscopy (TEM), and small angle X-ray (SAXS). The loading with dyes demonstrated. This fast open-vessel procedure gives access to amino acids-based nanomaterials potential applications in nanomedicine.

Language: Английский

Citations

7
Polypept(o)ides – Origins, Synthesis, Applications and Future Directions DOI Creative Commons
Tobias Bauer,

Leon Simić,

Joachim F. R. Van Guyse

et al.

Progress in Polymer Science, Journal Year: 2024, Volume and Issue: unknown, P. 101889 - 101889

Published: Sept. 1, 2024

Language: Английский

Citations

5
Design, Synthesis, and Evaluation of Camptothecin-Based Antibody–Drug Conjugates with High Hydrophilicity and Structural Stability DOI Creative Commons

Tianqin Xiong,

Jiyu Jin, Dongliang Liu

et al.

Molecules, Journal Year: 2025, Volume and Issue: 30(7), P. 1398 - 1398

Published: March 21, 2025

In this study, we constructed a linear antibody–drug conjugate (ADC), 7300-LP1003, by coupling the camptothecin derivative 095 to linker through an ether bond. vitro enzyme experiments indicated that LP1003 releases action of tissue cathepsin B. Therefore, introduced lysine pairs with different water-soluble substituents further modify and synthesized side-chain ADCs 7300-LP3004 7300-LP2004, modified polysarcosine polyethylene glycol, respectively. showed that, after incubation at 55 °C in phosphate-buffered saline for 48 h, aggregation was 45.24%, which significantly lower than 7300-LP1003 (77.14%). Cell cytotoxicity assays demonstrated ADCs, exhibited higher activity (IC50 values 39.74 nM 32.17 nM, respectively) compared ADC 7300-Deruxtecan 186.5 124.5 respectively). subcutaneous model SHP-77 NOD scid gamma mice, when dose 5 mg/kg, highest tumor inhibition rate growth (TGI) 106.09%, superior positive control 7300-Deruxtecan, had TGI 103.95%. conclusion, strong antitumor high physicochemical stability, highlighting need research development drugs.

Language: Английский

Citations

0
In Situ Formation of Nanoparticles from Graft Copolypeptides Under Dispersion Polymerization Conditions DOI
Ernesto Tinajero‐Díaz, Robert Murphy, Bo Li

et al.

Macromolecular Rapid Communications, Journal Year: 2025, Volume and Issue: unknown

Published: April 28, 2025

Abstract A simple method is presented for preparing polypeptide nanoparticles using hydrophilic biosynthetic ε‐poly(lysine) (εPL) as a reactive surfactant under dispersion polymerization conditions. In situ graft of benzyl‐L‐glutamic acid N ‐carboxyanhydride (BLG‐NCA) triggers the self‐assembly amphiphilic copolymers into nanoparticles, which are colloidally stabilized by remaining εPL amino groups at particle surface. The average nanoparticle diameter can be controlled in range 40–120 nm varying initiator‐to‐NCA ratio, demonstrated correlation between copolymer molecular weight (measured size exclusion chromatography) and z‐average dynamic light scattering). Secondary structure analysis indicates that α‐helical conformation poly(benzyl‐L‐glutamate) (PBLG) grafts plays role both accelerating NCA stabilizing nanostructures. This approach readily scalable to high concentrations offers straightforward route peptidomimetic entirely composed acids, with promising potential nanomedicine applications.

Language: Английский

Citations

0