Cationic Surface Charge Engineering of Recombinant Transthyretin Remarkably Increases the Inhibitory Potency Against Amyloid β-Protein Fibrillogenesis DOI Creative Commons
Xiaoding Lin,

Ting Xu,

Wenqi Hou

et al.

Molecules, Journal Year: 2024, Volume and Issue: 29(21), P. 5023 - 5023

Published: Oct. 24, 2024

The deposition of amyloid β-protein (Aβ) in the brain is main pathogenesis Alzheimer's disease (AD). development potent inhibitors against Aβ aggregation one effective strategies to combat AD. Endogenous transthyretin (TTR) can inhibit fibrillization via hydrophobic interactions, but its weak inhibitory potency hinders application AD therapy. Here, different recombinant TTRs were designed by cationic surface charge engineering. Compared with TTR, all positively charged showed enhanced capability inhibiting aggregation, especially protein obtained mutating acidic amino acid TTR arginine (TTR-nR) exhibited excellent effect. Among them, TTR-7R remarkably increased Aβ, which could effectively

Language: Английский

Where Biology Meets Engineering: Scaling Up Microbial Nutraceuticals to Bridge Nutrition, Therapeutics, and Global Impact DOI Creative Commons
Ahmed M. Elazzazy, Mohammed N. Baeshen,

Khalid M. Alasmi

et al.

Microorganisms, Journal Year: 2025, Volume and Issue: 13(3), P. 566 - 566

Published: March 2, 2025

The global nutraceutical industry is experiencing a paradigm shift, driven by an increasing demand for functional foods and dietary supplements that address malnutrition chronic diseases such as obesity, diabetes, cardiovascular conditions, cancer. Traditional plant- animal-derived nutraceuticals face limitations in scalability, cost, environmental impact, paving the way microbial biotechnology sustainable alternative. Microbial cells act bio-factories, converting nutrients like glucose amino acids into valuable products polyunsaturated fatty (PUFAs), peptides, other bioactive compounds. By harnessing their natural metabolic capabilities, microorganisms efficiently synthesize these compounds, making production effective approach development. This review explores transformative role of platforms nutraceuticals, emphasizing advanced fermentation techniques, synthetic biology, engineering. It addresses challenges optimizing strains, ensuring product quality, scaling while navigating regulatory frameworks. Furthermore, highlights cutting-edge technologies CRISPR/Cas9 genome editing, adaptive evolution strain enhancement, bioreactor innovations to enhance yield efficiency. With focus on sustainability precision, positioned game-changer industry, offering eco-friendly scalable solutions meet health needs. integration omics exploration novel sources hold potential revolutionize this field, aligning with growing consumer innovative products.

Language: Английский

Citations

2
What is the role of microbial biotechnology and genetic engineering in medicine? DOI Creative Commons
Fernando Santos‐Beneit

MicrobiologyOpen, Journal Year: 2024, Volume and Issue: 13(2)

Published: March 31, 2024

Abstract Microbial products are essential for developing various therapeutic agents, including antibiotics, anticancer drugs, vaccines, and enzymes. Genetic engineering techniques, functional genomics, synthetic biology unlock previously uncharacterized natural products. This review highlights major advances in microbial biotechnology, focusing on gene‐based technologies medical applications.

Language: Английский

Citations

13
Bioactivity and Bioavailability of Carotenoids Applied in Human Health: Technological Advances and Innovation DOI Open Access
Tomás Gabriel Bas

International Journal of Molecular Sciences, Journal Year: 2024, Volume and Issue: 25(14), P. 7603 - 7603

Published: July 11, 2024

This article presents a groundbreaking perspective on carotenoids, focusing their innovative applications and transformative potential in human health medicine. Research jointly delves deeper into the bioactivity bioavailability of revealing therapeutic uses technological advances that have to revolutionize medical treatments. We explore pioneering which carotenoids are used treat chronic diseases such as cancer, cardiovascular disease, age-related macular degeneration, offering novel protective mechanisms benefits. Our study also shows cutting-edge innovations carotenoid extraction bioavailability, including development supramolecular carriers advanced nanotechnology, dramatically improve absorption efficacy these compounds. These not only ensure consistent quality but tailor therapies each patient's needs, paving way for personalized By integrating latest scientific discoveries techniques, this research provides prospective clinical establishing new benchmark future studies field. findings underscore importance optimizing extraction, administration, bioactivity, methods develop more effective, targeted, treatments, thus visionary insight modern practices.

Language: Английский

Citations

12
Engineering Useful Microbial Species for Pharmaceutical Applications DOI Creative Commons
А.К. САДАНОВ, B.B. BAIMAKHANOVA,

Saltanat Emilievna Orasymbet

et al.

Microorganisms, Journal Year: 2025, Volume and Issue: 13(3), P. 599 - 599

Published: March 5, 2025

Microbial engineering has made a significant breakthrough in pharmaceutical biotechnology, greatly expanding the production of biologically active compounds, therapeutic proteins, and novel drug candidates. Recent advancements genetic engineering, synthetic biology, adaptive evolution have contributed to optimization microbial strains for applications, playing crucial role enhancing their productivity stability. The CRISPR-Cas system is widely utilized as precise genome modification tool, enabling enhancement metabolite biosynthesis activation biological pathways. Additionally, biology approaches allow targeted design microorganisms with improved metabolic efficiency potential, thereby accelerating development new products. integration artificial intelligence (AI) machine learning (ML) plays vital further advancing by predicting network interactions, optimizing bioprocesses, discovery process. However, challenges such efficient pathways, ensuring sustainable industrial-scale production, meeting international regulatory requirements remain critical barriers field. Furthermore, mitigate potential risks, it essential develop stringent biocontainment strategies implement appropriate oversight. This review comprehensively examines recent innovations analyzing key technological advancements, challenges, future perspectives.

Language: Английский

Citations

1
Analysis of meta-transcriptomics and identification of genes linked to bioactive peptides and vitamins in Indonesian tempe DOI

Jyoti Prakash Tamang,

Souvik Das, Sonam Lama

et al.

Food Research International, Journal Year: 2025, Volume and Issue: 202, P. 115757 - 115757

Published: Jan. 16, 2025

Language: Английский

Citations

0
Comprehensive Review on Enzymatic Polymer Degradation: A Sustainable Solution for Plastics DOI Creative Commons
M. Suresh,

Rafeeya Shams,

Kshirod Kumar Dash

et al.

Journal of Agriculture and Food Research, Journal Year: 2025, Volume and Issue: unknown, P. 101788 - 101788

Published: March 1, 2025

Language: Английский

Citations

0
Bacillus subtilis surface display technology: applications in bioprocessing and sustainable manufacturing DOI Creative Commons
Howra Bahrulolum,

Gholamreza Ahmadian

Biotechnology for Biofuels and Bioproducts, Journal Year: 2025, Volume and Issue: 18(1)

Published: March 15, 2025

The growing demand for sustainable and eco-friendly alternatives in bioprocessing, healthcare, manufacturing has stimulated significant interest Bacillus subtilis surface display technology. This innovative platform, leveraging both spore vegetative cell forms, provides exceptional versatility a wide spectrum of applications, spanning from green technologies to advanced biomedical innovations. robustness spores the metabolic activity cells enable efficient enzyme immobilization, biocatalysis, biosensor development, facilitating bioremediation, pollutant degradation, renewable energy generation. Additionally, B. systems have demonstrated remarkable potential vaccine development drug delivery, offering cost-effective, scalable, environmentally alternative traditional methods. These can effectively present antigens or therapeutic molecules, enabling targeted delivery robust immune responses. review explores recent advancements, challenges, opportunities harnessing technology biomanufacturing, innovations, transformative emphasizing its role addressing pressing global challenges environmental sustainability healthcare.

Language: Английский

Citations

0
Detoxification Techniques for Bacterial Toxins: A Pathway to Effective Toxoid Vaccines DOI
Parvaneh Esmaeilnejad-Ahranjani, Youcef Shahali, Maryam Dadar

et al.

Toxicon, Journal Year: 2025, Volume and Issue: 260, P. 108365 - 108365

Published: April 17, 2025

Language: Английский

Citations

0
Genetic Engineering in Bacteria, Fungi, and Oomycetes, Taking Advantage of CRISPR DOI Creative Commons

Piao Yang,

Abraham Condrich,

Ling Lü

et al.

DNA, Journal Year: 2024, Volume and Issue: 4(4), P. 427 - 454

Published: Nov. 14, 2024

Genetic engineering has revolutionized our ability to modify microorganisms for various applications in agriculture, medicine, and industry. This review examines recent advances genetic techniques bacteria, fungi, oomycetes, with a focus on CRISPR-Cas systems. In CRISPR-Cas9 enabled precise genome editing, enhancing antibiotic production metabolic engineering. For despite challenges associated their complex cell structures, CRISPR/Cas9 advanced the of enzymes secondary metabolites. significant plant pathogens, modified Agrobacterium-mediated transformation, CRISPR/Cas12a have contributed developing disease-resistant crops. provides comparative analysis efficiencies across these addresses ethical regulatory considerations. Future research directions include refining tools improve efficiency expand applicability non-model organisms. comprehensive overview highlights transformative potential microbiology its implications addressing global biotechnology.

Language: Английский

Citations

3
Advancing microbiota therapeutics: the role of synthetic biology in engineering microbial communities for precision medicine DOI Creative Commons
Asiya Nazir,

Fathima Hasnain Nadeem Hussain,

Afsheen Raza

et al.

Frontiers in Bioengineering and Biotechnology, Journal Year: 2024, Volume and Issue: 12

Published: Dec. 4, 2024

Over recent years, studies on microbiota research and synthetic biology have explored novel approaches microbial manipulation for therapeutic purposes. However, fragmented information is available this aspect with key insights scattered across various disciplines such as molecular biology, genetics, bioengineering, medicine. This review aims to the transformative potential of in advancing microbiome therapies, significant implications healthcare, agriculture, environmental sustainability. By merging computer science, engineering, allows precise design modification biological systems via cutting edge technologies like CRISPR/Cas9 gene editing, metabolic oligonucleotide synthesis, thus paving way targeted treatments personalized probiotics engineered microorganisms. The will also highlight vital role gut disorders caused by its dysbiosis suggesting microbiota-based therapies innovations biosensors real-time health monitoring, non-invasive diagnostic tools, automated bio foundries better outcomes. Moreover, challenges including genetic stability, safety, robust regulatory frameworks be discussed understand importance ongoing ensure safe effective interventions.

Language: Английский

Citations

3