Application of Bacteriocins in Practice, Including Microcins: the Present and the Future

Antibiot Khimioter = Antibiotics and Chemotherapy, Journal Year: 2025, Volume and Issue: 69(9-10), P. 71 - 85

Published: Feb. 10, 2025

In recent decades, bacteriocins have been actively studied as antimicrobial compounds. Bacteriocins primarily used to preserve food, but they are currently of increasing interest potential clinical agents and possible immunomodulatory agents. Infections caused by antibiotic-resistant bacteria declared a global public health threat. represent solution this worldwide threat due their broad- or narrow-spectrum activity against bacteria. particular, despite role natural alternatives chemical preservation in ensuring food safety, nisin remains the only bacteriocin approved regulatory authorities for preservation. The article highlights data on safety toxicity, which may become barrier wider use medicine industry. describes latest trends development bacteriocin-based drugs, effectiveness, spectrum activity, mechanisms action microorganisms.

Language: Английский

Turbidimetric bioassays: A solution to antimicrobial activity detection in asymptomatic bacteriuria isolates against uropathogenic Escherichia coli

MicrobiologyOpen, Journal Year: 2024, Volume and Issue: 13(3)

Published: May 6, 2024

Abstract Traditional bacteriocin screening methods often face limitations due to diffusion‐related challenges in agar matrices, which can prevent the peptides from reaching their target organism. Turbidimetric techniques offer a solution these issues, eliminating problems and providing an initial quantification of efficacy producer organisms. This study involved cell‐free supernatant (CFS) eight uncharacterized asymptomatic bacteriuria (ABU) isolates Escherichia coli 83972 for antimicrobial activity against clinical uropathogenic E. (UPEC) strains using turbidimetric growth methods. ABU exhibiting five or more UPEC were further characterized (PUTS 37, PUTS 58, 59, S‐07‐4, SK‐106‐1). The inhibition CFS by proteinase K suggested that was proteinaceous nature, potentially bacteriocins. 58 SK‐106‐1 enhanced artificial urine medium, with both inhibiting all UPECs. A putative microcin H47 operon identified SK‐106‐1, along previously V colicin E7 37 respectively. These findings indicate bacteriocin‐producers could serve as viable prophylactics therapeutics increasing antibiotic resistance among uropathogens.

Language: Английский

Genotypic and phenotypic characterisation of asymptomatic bacteriuria (ABU) isolates displaying bacterial interference against multi-drug resistant uropathogenic E. Coli

Archives of Microbiology, Journal Year: 2024, Volume and Issue: 206(10)

Published: Sept. 9, 2024

Language: Английский