RBM15-dependent m6A modification mediates progression of non-small cell lung cancer cells DOI Creative Commons
Man Wang,

Ya‐Zhen Qin,

Xiaoqi Ai

et al.

Molecular Medicine, Journal Year: 2024, Volume and Issue: 30(1)

Published: Dec. 23, 2024

Non-small cell lung cancer (NSCLC) is the predominant form of cancer, contributing significantly to global health and economic challenges. This study elucidated role RBM15 in NSCLC progression through its involvement m6A modifications. levels tissues cells were assessed via RT-qPCR Western blotting. The impact knockdown on proliferation, invasion, migration was evaluated using CCK-8, colony formation, Transwell assays. Expression KLF1, TRIM13, ANXA8 determined by blot. methylation analyzed, while RIP MeRIP assays employed explore interaction between YTHDF1/YTHDF2/m6A KLF1/TRIM13, as well KLF1 binding promoter. ubiquitination examined Xenograft metastasis models utilized assess RBM15's vivo. found be overexpressed NSCLC. Silencing led decreased cells. upregulated downregulated TRIM13 YTHDF1/YTHDF2, resulting promotion expression. overexpression or downregulation partially reversed suppressive effects proliferation. ANXA8, NSCLC, mitigated inhibitory silencing malignant behaviors. In vivo, hindered proliferation modulating KLF1-TRIM13/ANXA8 axis. RBM15-mediated enhances expression suppresses thereby promoting facilitating progression. These findings provide novel insights potential therapeutic targets for treatment.

Language: Английский

The promoting effect of the POU3F2/METTL16/PFKM cascade on glycolysis and tumorigenesis of hepatocellular carcinoma DOI Creative Commons

Ming Chen,

Yuan Yang,

Guangsheng Hu

et al.

Annals of Hepatology, Journal Year: 2025, Volume and Issue: unknown, P. 101776 - 101776

Published: Jan. 1, 2025

Deregulation of m

Language: Английский

Citations

1
Emerging importance of m6A modification in liver cancer and its potential therapeutic role DOI
Tao Chen,

Wufei Ye,

Songsen Gao

et al.

Biochimica et Biophysica Acta (BBA) - Reviews on Cancer, Journal Year: 2025, Volume and Issue: unknown, P. 189299 - 189299

Published: March 1, 2025

Language: Английский

Citations

0
Hepatitis B Virus Promotes Angiogenesis in Hepatocellular Carcinoma by Increasing m6A Modification of VEGFA mRNA via IGF2BP3 DOI
Xiaoxin Xu, Yi Zhang,

Shuxiang Wu

et al.

Journal of Medical Virology, Journal Year: 2025, Volume and Issue: 97(5)

Published: April 22, 2025

ABSTRACT Angiogenesis plays a crucial role in the development of HBV‐related hepatocellular carcinoma (HCC). VEGFA is key angiogenic factor, and while its transcriptional regulation by HBV has been extensively studied, posttranscriptional remains poorly understood. Building on our previous findings that delineated an RBM15/YTHDF2/IGF2BP3 regulatory axis m6A‐mediated RNA metabolism HCC, this study further explores HBV. By MeRIP‐qPCR integrating MeRIP‐seq data, we discovered enhances m6A methylation mRNA. Comprehensive cellular molecular biology experiments demonstrated induces upregulation IGF2BP3, which serves as “reader” recognizes stabilizes mRNA methylation‐dependent manner. This stabilization leads to elevated expression, promoting enhanced functions such HUVEC migration tube formation. Furthermore, HBV‐associated HCC xenograft model, IGF2BP3 knockdown resulted decreased expression inhibited tumor growth. expands understanding HBV‐driven angiogenesis identifies IGF2BP3‐VEGFA potential therapeutic target for antiangiogenic strategies HCC.

Language: Английский

Citations

0
Deciphering the IGF2BP3-mediated control of ferroptosis: mechanistic insights and therapeutic prospects DOI Creative Commons
Wenjuan Zhang, Hongxing Liu,

Changrong Ren

et al.

Discover Oncology, Journal Year: 2024, Volume and Issue: 15(1)

Published: Oct. 11, 2024

The rapid expansion of the oncology field has revealed new insights into cell death processes, with a particular emphasis on role ferroptosis. Characterized by iron dependency and accumulation lipid peroxides within cells, ferroptosis stands out as unique form programmed demise. This in-depth analysis delves pivotal IGF2BP3, an RNA-binding protein, in complex regulatory network cancerous cells. By exerting post-transcriptional control over genes associated equilibrium, IGF2BP3 is demonstrated to manage cellular concentrations reactive oxygen species (ROS) thus affecting destiny cell. correlation between aberrant expression increased aggressiveness, metastatic capacity, poor prognosis various cancers further clarified. potential biomarker for therapeutic agent enhance cancer cells' vulnerability also examined, heralding strategies treatment.

Language: Английский

Citations

0
RBM15-dependent m6A modification mediates progression of non-small cell lung cancer cells DOI Creative Commons
Man Wang,

Ya‐Zhen Qin,

Xiaoqi Ai

et al.

Molecular Medicine, Journal Year: 2024, Volume and Issue: 30(1)

Published: Dec. 23, 2024

Non-small cell lung cancer (NSCLC) is the predominant form of cancer, contributing significantly to global health and economic challenges. This study elucidated role RBM15 in NSCLC progression through its involvement m6A modifications. levels tissues cells were assessed via RT-qPCR Western blotting. The impact knockdown on proliferation, invasion, migration was evaluated using CCK-8, colony formation, Transwell assays. Expression KLF1, TRIM13, ANXA8 determined by blot. methylation analyzed, while RIP MeRIP assays employed explore interaction between YTHDF1/YTHDF2/m6A KLF1/TRIM13, as well KLF1 binding promoter. ubiquitination examined Xenograft metastasis models utilized assess RBM15's vivo. found be overexpressed NSCLC. Silencing led decreased cells. upregulated downregulated TRIM13 YTHDF1/YTHDF2, resulting promotion expression. overexpression or downregulation partially reversed suppressive effects proliferation. ANXA8, NSCLC, mitigated inhibitory silencing malignant behaviors. In vivo, hindered proliferation modulating KLF1-TRIM13/ANXA8 axis. RBM15-mediated enhances expression suppresses thereby promoting facilitating progression. These findings provide novel insights potential therapeutic targets for treatment.

Language: Английский

Citations

0