Extracellular Vesicles from Adipose‐Derived Mesenchymal Stem Cells Combined with PEG Hydrogel Alleviate Maternal Simulated Birth Injury in a Rat Model DOI
Qīng Wáng, Yaqin Li, Yue Zhang

et al.

Advanced Healthcare Materials, Journal Year: 2025, Volume and Issue: unknown

Published: April 16, 2025

Abstract Pelvic organ prolapse (POP) is a common and distressing condition affecting women, particularly those with history of vaginal delivery. The impact extracellular vesicles derived from adipose‐derived mesenchymal stem cells (ADSC‐EVs) on pelvic floor tissue injury remains unclear. Due to their short half‐life rapid clearance in vivo, ADSC‐EVs lose efficacy quickly. To address this, an injectable tetra‐PEG hydrogel encapsulate (PEG@EVs) developed. formed by tetra‐PEG‐NH 2 tetra‐PEG‐NHS through ammonolysis reaction, leading the formation amide bonds within seconds. Vaginal wall POP patients shows disruption matrix, lipid peroxidation, inflammation. In vitro, significantly reduce H₂O₂‐induced oxidative stress, oxidation, apoptosis, while enhancing expression Nrf2 its downstream targets—CAT, NQO1, HO‐1, SOD2. also upregulate GPX4 SLC7A11, reducing mitochondrial damage mitigating ferroptosis. inhibitor ML385 reverses these protective effects. rat model childbirth injury, PEG@EVs treatment promotes nuclear translocation, induces M1‐to‐M2 macrophage conversion, reduces inflammation, stimulates collagen deposition, thereby accelerating repair. findings this study may serve as foundation for early targeted intervention POP, representing promising therapeutic approach.

Language: Английский

Recent Advances and Challenges for Biological Materials in Micro/Nanocarrier Synthesis for Bone Infection and Tissue Engineering DOI
Qinglin Xia,

Shuyan Zhou,

Jingya Zhou

et al.

ACS Biomaterials Science & Engineering, Journal Year: 2025, Volume and Issue: unknown

Published: March 11, 2025

Roughly 1.71 billion people worldwide suffer from large bone abnormalities, which are the primary cause of disability. Traditional grafting procedures have several drawbacks that impair their therapeutic efficacy and restrict use in clinical settings. A great deal work has been done to create fresh, more potent strategies. Under these circumstances, a crucial technique for regeneration major lesions emerged: tissue engineering (BTE). BTE involves biomaterials can imitate natural design bone. To yet, no biological material able fully meet parameters perfect implantable material, even though varieties created investigated regeneration. Against this backdrop, researchers focused interest over past few years on subject nanotechnology nanostructures regenerative medicine. The ability nanoengineered particles overcome current constraints strategies─such as decreased cell proliferation differentiation, insufficient mechanical strength materials, production extrinsic factors required effective osteogenesis revolutionized field engineering. effects nanoparticles characteristics application materials main topics our review, summarizes most recent vitro vivo research context BTE.

Language: Английский

Citations

0
Evaluation of Regeneration Potential of Bone Marrow–Derived Mesenchymal Stem Cells on Induced Damaged Submandibular Salivary Gland in Mice DOI Creative Commons
Nadia Radi, Doaa Habba, Seham I. Hallool

et al.

European Journal of Dentistry, Journal Year: 2025, Volume and Issue: unknown

Published: March 12, 2025

Abstract Objectives The ultimate goal of stem cell (SC) transplantation is the regeneration salivary gland function by transplanted SCs differentiating into cells. Therefore, this study aimed to evaluate regenerative capacity bone marrow–derived mesenchymal cells (BM-MSCs) in irradiated mice using immunohistochemical markers Ki-67 and CD34. Material Methods Four groups male were included study. Group I (normal control) comprised six that not subjected gamma radiation. II treated with BM-MSCs. III 12 intraglandular injection labeled BM-MSCs their submandibular glands, 24 hours postradiation. IV on day 11 Statistical Analysis Data presented as mean standard deviation. different compared a one-way analysis variance (ANOVA). Results ANOVA test revealed difference between all was extremely statistically significant (p < 0.003), Tukey's post hoc group I, III, regarding microvessel density CD34 immunoexpression groups. Conclusion have potential induced damaged glands mice; time an essential factor

Language: Английский

Citations

0
Advancements in Skin Tissue Regeneration: Unveiling Emerging Treatment Strategies, Regulatory Perspectives, and Future Directions for Enhanced Healing DOI

Pawan Jaiswal,

Giriraj Pandey,

Shivam Kumar

et al.

Journal of Drug Delivery Science and Technology, Journal Year: 2025, Volume and Issue: unknown, P. 106872 - 106872

Published: April 1, 2025

Language: Английский

Citations

0
A Versatile Immune Protective Armor to Enhance the Regenerative Potential of Exogenous Stem Cells DOI

Rongbai Ju,

Xinghui Gao,

Chi Zhang

et al.

ACS Applied Materials & Interfaces, Journal Year: 2025, Volume and Issue: unknown

Published: April 8, 2025

Host immune rejection has long been recognized as a major contributor to the poor survival rates of exogenous stem cells (ESCs). In this study, we present simple and versatile strategy protect ESCs from host system insults by developing protective "armor." This armor was designed using tannic acid (TA), leveraging its strong affinity for biomacromolecules anti-inflammatory properties. Prior implantation, can be readily applied surface individual ESCs, cell aggregates, sheets, or cell-laden hydrogel systems simply immersing them in TA solution several seconds, without additional processing steps. The TA-based effectively modulates acute inflammatory response during initial days postimplantation scavenging reactive oxygen species (ROS), thereby creating an ESCs-friendly microenvironment. evidenced reduction infiltration pro-inflammatory secretion cytokines. Consequently, engrafted significantly enhanced, with preserved stemness immunomodulatory functions. regenerative potential further demonstrated rat periodontal defect model. These findings provide novel approach enhancing performance offer straightforward shield rejection.

Language: Английский

Citations

0
Extracellular Vesicles from Adipose‐Derived Mesenchymal Stem Cells Combined with PEG Hydrogel Alleviate Maternal Simulated Birth Injury in a Rat Model DOI
Qīng Wáng, Yaqin Li, Yue Zhang

et al.

Advanced Healthcare Materials, Journal Year: 2025, Volume and Issue: unknown

Published: April 16, 2025

Abstract Pelvic organ prolapse (POP) is a common and distressing condition affecting women, particularly those with history of vaginal delivery. The impact extracellular vesicles derived from adipose‐derived mesenchymal stem cells (ADSC‐EVs) on pelvic floor tissue injury remains unclear. Due to their short half‐life rapid clearance in vivo, ADSC‐EVs lose efficacy quickly. To address this, an injectable tetra‐PEG hydrogel encapsulate (PEG@EVs) developed. formed by tetra‐PEG‐NH 2 tetra‐PEG‐NHS through ammonolysis reaction, leading the formation amide bonds within seconds. Vaginal wall POP patients shows disruption matrix, lipid peroxidation, inflammation. In vitro, significantly reduce H₂O₂‐induced oxidative stress, oxidation, apoptosis, while enhancing expression Nrf2 its downstream targets—CAT, NQO1, HO‐1, SOD2. also upregulate GPX4 SLC7A11, reducing mitochondrial damage mitigating ferroptosis. inhibitor ML385 reverses these protective effects. rat model childbirth injury, PEG@EVs treatment promotes nuclear translocation, induces M1‐to‐M2 macrophage conversion, reduces inflammation, stimulates collagen deposition, thereby accelerating repair. findings this study may serve as foundation for early targeted intervention POP, representing promising therapeutic approach.

Language: Английский

Citations

0