Exploring the role of LOX family in glioma progression and immune modulation

Frontiers in Immunology, Journal Year: 2025, Volume and Issue: 16

Published: April 9, 2025

Glioma is a major cause of mortality among central nervous system tumors, with generally poor prognosis. The lysyl oxidase (LOX) family, group copper-dependent amine oxidases, has been implicated in the progression various cancers, but its specific role glioma and relationship immune infiltration remains insufficiently explored. This study aims to investigate LOX family's expression, prognostic significance, dynamics identify potential therapeutic targets. A comprehensive analysis was conducted using public databases assess gene mutation frequency, patterns related family glioma. results were validated through survival immunohistochemistry. Functional assays, including EdU, Transwell, flow cytometry, used evaluate cell proliferation, migration, invasion, apoptosis. Co-culture experiments cells, ELISA, transplantation model employed immune-modulatory effects family. Gene protein expression levels further analyzed qRT-PCR Western blotting. significantly upregulated low-grade gliomas strongly associated clinical outcomes. Although frequencies low, contributed pathways involving metastasis, hypoxia response, angiogenesis, infiltration. correlated increased macrophages eosinophils decreased presence Treg CD8+ T cells. Knockdown genes impaired functions, induced apoptosis, altered behavior by reducing M2 macrophage polarization enhancing activity. overexpressed prognosis patterns. These findings highlight as promising marker target, particularly for effectiveness immunotherapy treatment.

Language: Английский

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LINC02774 inhibits glycolysis in glioma to destabilize HIF‐1α dependent on transcription factor RP58

MedComm, Journal Year: 2023, Volume and Issue: 4(5)

Published: Sept. 11, 2023

Glioma, the most common of malignant tumors in brain, is responsible for majority deaths from primary brain tumors. The regulation long noncoding RNAs (lncRNAs) HIF-1α-driven tumor development remains unclear. LINC02774 a nuclear lncRNA and that it being reported first time this study. We found downregulation glioma decreased with degree malignant, its expression showing negative correlation relative index enhanced magnetic resonance (RIEMR). RIEMR-associated was to inhibit glycolysis by modulating hypoxia pathway rather than response itself. interacted neighboring gene, RP58 (ZBTB18), enhance PHD3, which catalyzed HIF-1α hydroxylase ubiquitination, leading expression. also function LINC02774, dependent on diminished upon depletion. Notably, higher associated favorable prognosis. In conclusion, these findings reveal role relies neighbor RP58, regulate as novel suppressor, suggesting potential be prognostic marker molecular target therapy glioma.

Language: Английский

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Feedback loop LINC00511–YTHDF2–SOX2 regulatory network drives cholangiocarcinoma progression and stemness

MedComm, Journal Year: 2024, Volume and Issue: 5(11)

Published: Oct. 22, 2024

Abstract Cholangiocarcinoma (CCA) was identified as a malignant tumor with rising incidence and mortality rates, the roles of long noncoding RNA (lncRNA) in CCA remained not entirely clear. In this study, LINC00511 had high expression CCA, which closely related to poor prognosis. Knockdown significantly inhibited cell biological behaviors. It also affected stemness evidenced by decreased SOX2 protein expression. Moreover, study revealed interaction LINC00511, YTHDF2, CCA. Specifically, facilitated formation complex YTHDF2 on mRNA, uniquely enhances mRNA's stability through m6A methylation sites. This stabilization appears crucial for maintaining behaviors cells. Additionally, modulated via PI3K/AKT signaling pathway. Meanwhile, can promote an upstream transcription factor, thereby confirming positive feedback loop formed SOX2, plays significant role occurrence development Finally, successfully constructed two patient‐derived xenograft models, revealing vital development. summary, research provides comprehensive understanding pathogenesis

Language: Английский

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Glucose metabolism in glioma: an emerging sight with ncRNAs

Cancer Cell International, Journal Year: 2024, Volume and Issue: 24(1)

Published: Sept. 13, 2024

Language: Английский

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