Intranasal Inoculation of Cationic Crosslinked Carbon Dots‐Adjuvanted Respiratory Syncytial Virus F Subunit Vaccine Elicits Mucosal and Systemic Humoral and Cellular Immunity

MedComm, Journal Year: 2025, Volume and Issue: 6(4)

Published: March 24, 2025

ABSTRACT Respiratory syncytial virus (RSV) causes severe acute lower respiratory tract infections, especially in infants and the elderly. Developing an RSV vaccine that promotes a robust mucosal immune response is necessary to successfully prevent viral transmission development of disease. We previously reported crosslinked carbon dots (CCD) may be excellent adjuvant candidate for intranasal (IN) protein subunit vaccines. Considering strong immunogenicity prefused F (preF), we prepared IN composed CCD preF as antigen (CCD/preF) evaluated induced antigen‐specific humoral cellular immunity. found immunization with CCD/preF elicited serum IgG responses immunity, including secreted IgA antibodies, tissue‐resident memory T (T RM ) cells, B which lasted at least 1 year. In addition, combination intramuscular stronger systemic Together, this study proved high vaccines supported university adjuvant, supporting further larger animal models clinical studies.

Language: Английский

AH-6809 mediated regulation of lung adenocarcinoma metastasis through NLRP7 and prognostic analysis of key metastasis-related genes

Frontiers in Pharmacology, Journal Year: 2024, Volume and Issue: 15

Published: Dec. 4, 2024

Lung adenocarcinoma (LUAD) has become one of the leading causes cancer-related deaths globally, with metastasis representing most lethal stage disease. Despite significant advances in diagnostic and therapeutic strategies for LUAD, mechanisms enabling cancer cells to breach blood-brain barrier remain poorly understood. While genomic profiling shed light on nature primary tumors, genetic drivers clinical relevance LUAD are still largely unexplored.

Language: Английский

Paromomycin targets HDAC1-mediated SUMOylation and IGF1R translocation in glioblastoma

Frontiers in Pharmacology, Journal Year: 2024, Volume and Issue: 15

Published: Dec. 11, 2024

Objective This study investigates the effects of Paromomycin on SUMOylation-related pathways in glioblastoma (GBM), specifically targeting HDAC1 inhibition. Methods Using TCGA and GTEx datasets, we identified genes associated with GBM prognosis. Molecular docking analysis suggested as a potential inhibitor. In vitro assays U-251MG cells were performed to assess Paromomycin’s cell viability, SUMOylation gene expression, IGF1R translocation using CCK8 assays, qRT-PCR, immunofluorescence. Results treatment led dose-dependent reduction colony formation, migration. It modulated SUMO1 expression decreased nuclear translocation, an effect reversible by inhibitor Trochostatin A (TSA), suggesting involvement SUMO1-regulated pathways. Conclusion highlights therapeutic agent for HDAC1-mediated influencing warranting further investigation its clinical application.

Language: Английский