Exploring the effect of soybean fermentation broth (S-FB) on gut microbes of lipopolysaccharide (LPS)-infected loach (Misgurnus anguillicaudatus) using 16S rRNA sequencing

Frontiers in Microbiology, Journal Year: 2025, Volume and Issue: 16

Published: March 18, 2025

The fermentation products of soybean are rich in beneficial bacteria, which play Shenghui Chu a significant role maintaining the balance intestinal microbiota and improving health. To investigate immunomodulatory effects broth (S-FB) on loach (Misgurnus anguillicaudatus) with lipopolysaccharide (LPS)-induced enteritis, 16S rDNA high-throughput sequencing technology was employed to analyze composition structure two groups: LPS-treated group (fed broth) control (normal feeding conditions). results revealed that relative abundance such as Lactobacillus Muribaculaceae, significantly increased treatment group, while harmful including Aeromonas Shewanella, decreased. These findings suggest can repair damage maintain health by enhancing bacteria reducing pathogenic host. Functional prediction studies microbial communities also showed groups primarily affected metabolic genetic information processing. research analyzed changes distribution microflora different loach, providing new insights into possible liquid inflammation.

Language: Английский

Mung Bean Peptides Alleviate Dextran-Sulfate-Sodium-Induced Colitis Symptoms in Mice by Protecting the Intestinal Mechanical Barrier and Regulating Gut Microbiota

Foods, Journal Year: 2025, Volume and Issue: 14(8), P. 1363 - 1363

Published: April 15, 2025

Ulcerative colitis (UC), an idiopathic and recurrent ailment, substantially influences a patient’s health. Mung bean peptides (MBPs) are bioactive substances derived from mung protein that possess notable anti-inflammatory properties. However, their efficacy underlying mechanisms in UC treatment remain unclear. In this study, the structural characteristics of MBPs were examined by determining various parameters, such as amino acid composition, molecular weight distribution, peptide sequences, thereby structurally demonstrating potential. The therapeutic effectiveness was evaluated assessing its influence on colon length, histological damage to colonic tissue, disease activity index mice suffering induced dextran sulfate sodium (DSS). Additionally, study explored potential mechanism action analyzing intestinal microbiota, inflammatory cytokines serum, tight junction (TJ) proteins tissue mice. results revealed significantly increased reduced damage, decreased with UC. restored barrier function upregulating expression ZO-1 claudin-1 within DSS-induced colitis, treating exerted effects downregulating amplification improving gut microbiota structure regulating immune-related signaling pathways. Therefore, there is experimental basis for use adjunctive therapy

Language: Английский