Unleashing the Healing Power: 3D Bioprinting Mimics Hypoxia to Supercharge Mesenchymal Stem Cells
ACS Applied Materials & Interfaces,
Journal Year:
2025,
Volume and Issue:
unknown
Published: March 2, 2025
Mesenchymal
stem
cells
(MSCs)
play
a
critical
role
in
cell
therapy
due
to
their
tissue-mimicking
abilities.
However,
conventional
2D
culture
conditions
often
lead
the
loss
of
native
hypoxic
niche,
potentially
limiting
therapeutic
efficacy.
3D
bioprinting
offers
method
recreate
intricate
biological
environments
by
integrating
with
extracellular
matrices.
Therefore,
it
is
essential
adapt
printing
techniques
accurately
replicate
MSCs'
ecological
facilitating
integration
technology
into
clinical
applications.
In
this
study,
we
optimized
capabilities
using
performed
cellular
aggregates
(PCA)
method.
We
observed
that
printed
matrix
creates
microenvironment,
resulting
significant
increase
level
production
several
paracrine
signaling
molecules
and
immunomodulatory
factors
MSCs.
Furthermore,
MSCs
exhibited
enhanced
stemness
proliferative
capacity
early
stages
culture.
RNA-seq
analysis
revealed
these
changes
behavior
were
associated
environment
created
during
procedure
By
optimizing
bioink
composition
parameters,
successfully
simulated
vivo
leading
notable
improvements
MSC
characteristics
capacity.
RNA
sequencing
further
confirmed
activation
hypoxia
pathways,
which
are
crucial
for
properties.
These
findings
offer
valuable
insights
leveraging
MSC-based
therapies
regenerative
medicine.
Language: Английский
Regenerative Medicine in Plastic Surgery: The Role of Stem Cells and Bioprinting
Regenesis repair rehabilitation.,
Journal Year:
2025,
Volume and Issue:
unknown
Published: March 1, 2025
Language: Английский
Review on machine learning application in tissue engineering: What has been done so far? Application areas, challenges, and perspectives
Journal of Materials Science,
Journal Year:
2024,
Volume and Issue:
unknown
Published: Nov. 23, 2024
Language: Английский
Trends in 3D Printing Processes for Biomedical Field: Opportunities and Challenges: An Editorial Retrospective
Biomedicines,
Journal Year:
2024,
Volume and Issue:
12(11), P. 2612 - 2612
Published: Nov. 15, 2024
On
8
August
1984,
Chuck
Hull
(Charles
W
[...].
Language: Английский