Electrobiocorrosion by Microbes without Outer-Surface Cytochromes DOI Open Access
Dawn E. Holmes,

Trevor L. Woodard,

Jessica A. Smith

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2023, Volume and Issue: unknown

Published: July 26, 2023

Abstract Anaerobic microbial corrosion of iron-containing metals causes extensive economic damage. Some microbes are capable direct metal-to-microbe electron transfer (electrobiocorrosion), but the prevalence electrobiocorrosion among diverse methanogens and acetogens is poorly understood because a lack tools for their genetic manipulation. Previous studies have suggested that respiration with 316L stainless steel as donor indicative because, unlike pure Fe 0 , does not abiotically generate H 2 an intermediary carrier. Here we report all ( Methanosarcina vacuolata Methanothrix soehngenii Methanobacterium strain IM1) Sporomusa ovata Clostridium ljungdahlii ) evaluated respired donor, only M. Mx S. were electrobiocorrosion. The electrobiocorrosive required acetate additional energy source in order to produce methane from steel. Co-cultures Mx. demonstrated how can provide during corrosion. Not was IM1 electrobiocorrosion, it also did accept electrons Geobacter metallireducens effective electron- donating partner interspecies directly . finding despite outer-surface c -type cytochromes previously found be important other microbes, demonstrates there multiple strategies making electrical contact Impact Statement Understanding anaerobic receive likely lead novel mitigating metals, which has enormous impact. Electrobiocorrosion, relatively recently recognized mechanism. It cultures when oxidation inhibited by deletion genes known involved forms extracellular exchange. However, many obvious connections difficult genetically manipulate. study reported here provides alternative approach evaluating whether require results indicate IM1, electrobiocorrosive, contrast previous speculation. some without do appear suggesting this mechanism may more widespread than thought.

Language: Английский

Microbially mediated metal corrosion DOI
Dake Xu, Tingyue Gu, Derek R. Lovley

et al.

Nature Reviews Microbiology, Journal Year: 2023, Volume and Issue: 21(11), P. 705 - 718

Published: June 21, 2023

Language: Английский

Citations

215
Accelerated Microbial Corrosion by Magnetite and Electrically Conductive Pili through Direct Fe0‐to‐Microbe Electron Transfer DOI
Yuting Jin,

Enze Zhou,

Toshiyuki Ueki

et al.

Angewandte Chemie International Edition, Journal Year: 2023, Volume and Issue: 62(38)

Published: Aug. 1, 2023

Electrobiocorrosion, the process in which microbes extract electrons from metallic iron (Fe0 ) through direct Fe0 -microbe electrical connections, is thought to contribute costly corrosion of iron-containing metals that impacts many industries. However, electrobiocorrosion mechanisms are poorly understood. We report here electrically conductive pili (e-pili) and mineral magnetite play an important role electron transfer between Geobacter sulfurreducens, first microbe has been rigorously documented. Genetic modification express substantially diminished corrosive pitting rates -to-microbe flux. Magnetite reduced resistance transfer, increasing currents intensifying pitting. Studies with mutants suggested promoted a manner similar outer-surface c-type cytochrome OmcS. These findings, fact common product corrosion, suggest potential positive feedback loop produced during further accelerating electrobiocorrosion. The interactions e-pili, cytochromes, demonstrate mechanistic complexities electrobiocorrosion, but also provide insights into detecting possibly mitigating this economically damaging process.

Language: Английский

Citations

37
Burning question: Are there sustainable strategies to prevent microbial metal corrosion? DOI Creative Commons
Di Wang,

Enze Zhou,

Dake Xu

et al.

Microbial Biotechnology, Journal Year: 2023, Volume and Issue: 16(11), P. 2026 - 2035

Published: Oct. 5, 2023

Abstract The global economic burden of microbial corrosion metals is enormous. Microbial iron‐containing most extensive under anaerobic conditions. Microbes form biofilms on metal surfaces and can directly extract electrons derived from the oxidation Fe 0 to 2+ support respiration. H 2 generated abiotic also serves as an electron donor for respiratory microbes. metabolites accelerate this oxidation. Traditional strategies curbing include cathodic protection, scrapping, a diversity biocides, alloys that protective layers or release toxic ions, polymer coatings. However, these approaches are typically expensive and/or limited applicability not environmentally friendly. Biotechnology may provide more effective sustainable solutions. Biocides produced with microbes be less eukaryotes, expanding environments potential application. Microbially surfactants diminish biofilm formation by corrosive microbes, quorum‐sensing inhibitors. Amendments phages predatory bacteria have been successful in attacking laboratory studies. Poorly deposit extracellular polysaccharides minerals protect surface their metabolites. Nitrate amendments permit nitrate reducers outcompete highly sulphate‐reducing reducing corrosion. Investigation all mitigation its infancy. More study, especially relevant conditions, including diverse communities, warranted.

Language: Английский

Citations

23
Unveiling the unique role of iron in the metabolism of methanogens: A review DOI
Yuxin Ma, Ying Qu,

Xiangwu Yao

et al.

Environmental Research, Journal Year: 2024, Volume and Issue: 250, P. 118495 - 118495

Published: Feb. 15, 2024

Language: Английский

Citations

11
MmcA is an electron conduit that facilitates both intracellular and extracellular electron transport in Methanosarcina acetivorans DOI Creative Commons
Dinesh Gupta, Keying Chen, Sean J. Elliott

et al.

Nature Communications, Journal Year: 2024, Volume and Issue: 15(1)

Published: April 17, 2024

Abstract Methanogens are a diverse group of Archaea that obligately couple energy conservation to the production methane. Some methanogens encode alternate pathways for conservation, like anaerobic respiration, but biochemical details this process unknown. We show multiheme c -type cytochrome called MmcA from Methanosarcina acetivorans is important intracellular electron transport during methanogenesis and can also reduce extracellular acceptors soluble Fe 3+ anthraquinone-2,6-disulfonate. Consistent with these observations, displays reversible redox features ranging −100 −450 mV versus SHE. Additionally, mutants lacking mmcA have significantly slower reduction rates. The locus prevalent in members Order Methanosarcinales part distinct clade cytochromes closely related octaheme tetrathionate reductases. Taken together, might act as an conduit potentially support variety strategies extend beyond methanogenesis.

Language: Английский

Citations

9
Cytochrome‐mediated direct electron uptake from metallic iron by Methanosarcina acetivorans DOI Creative Commons
Dawn E. Holmes,

Haiyan Tang,

Trevor L. Woodard

et al.

mLife, Journal Year: 2022, Volume and Issue: 1(4), P. 443 - 447

Published: Nov. 17, 2022

Impact statement Methane‐producing microorganisms accelerate the corrosion of iron‐containing metals. Previous studies have inferred that some methanogens might directly accept electrons from Fe(0), but when this possibility was more intensively investigated, H 2 shown to be an intermediary electron carrier between Fe(0) and methanogens. Here, we report Methanosarcina acetivorans catalyzes direct metal‐to‐microbe transfer support methane production. Deletion gene for multiheme, outer‐surface c ‐type cytochrome MmcA eliminated production consistent with key role in other forms extracellular exchange. These findings, coupled previous demonstration cytochromes are also electrical contacts uptake by Geobacter Shewanella species, suggest presence multiheme on surfaces diagnostic interfering function a strategy mitigate corrosion.

Language: Английский

Citations

32
H 2 Is a Major Intermediate in Desulfovibrio vulgaris Corrosion of Iron DOI Creative Commons

Trevor L. Woodard,

Toshiyuki Ueki,

Derek R. Lovley

et al.

mBio, Journal Year: 2023, Volume and Issue: 14(2)

Published: Feb. 14, 2023

Desulfovibrio vulgaris has been a primary pure culture sulfate reducer for developing microbial corrosion concepts. Multiple mechanisms how it accepts electrons from Fe

Language: Английский

Citations

22
Electrobiocorrosion by microbes without outer‐surface cytochromes DOI Creative Commons
Dawn E. Holmes,

Trevor L. Woodard,

Jessica A. Smith

et al.

mLife, Journal Year: 2024, Volume and Issue: 3(1), P. 110 - 118

Published: March 1, 2024

Abstract Anaerobic microbial corrosion of iron‐containing metals causes extensive economic damage. Some microbes are capable direct metal‐to‐microbe electron transfer (electrobiocorrosion), but the prevalence electrobiocorrosion among diverse methanogens and acetogens is poorly understood because a lack tools for their genetic manipulation. Previous studies have suggested that respiration with 316L stainless steel as donor indicative electrobiocorrosion, because, unlike pure Fe 0 , does not abiotically generate H 2 an intermediary carrier. Here, we report all ( Methanosarcina vacuolata, Methanothrix soehngenii Methanobacterium strain IM1) Sporomusa ovata Clostridium ljungdahlii ) evaluated respired donor, only M. Mx. S. were electrobiocorrosion. The electrobiocorrosive required acetate additional energy source in order to produce methane from steel. Cocultures demonstrated how can provide during corrosion. Not was IM1 it also did accept electrons Geobacter metallireducens effective electron‐donating partner interspecies directly . finding despite outer‐surface c ‐type cytochromes previously found be important other microbes, demonstrates there multiple strategies making electrical contact

Language: Английский

Citations

8
Chloride enhances corrosion associated with sulfate-reducing bacteria DOI
Di Wang,

Toshiyuki Ueki,

Peiyu Ma

et al.

Corrosion Science, Journal Year: 2024, Volume and Issue: 233, P. 112096 - 112096

Published: May 3, 2024

Language: Английский

Citations

7
Fe0-dependent carbon dioxide reduction to methane via diverse electron transfer pathway in methanogenic community DOI Creative Commons
Tianyu Gao, Lichao Xia, Hanmin Zhang

et al.

Cell Reports Sustainability, Journal Year: 2024, Volume and Issue: 1(2), P. 100019 - 100019

Published: Feb. 1, 2024

In natural and engineered environments, iron biocorrosion is an energy reservoir for growth of methanogens. However, how archaea accept electrons from metallic remains enigmatic. Here, we report that a Methanothrix-dominated methanogenic community anaerobic granular sludge can reduce carbon dioxide (CO2) to methane (CH4) via electron uptake zero-valent (ZVI). Through the batch experiments, maximum CH4 yield 40.8 ± 0.6 μeequiv/day recovery ZVI oxidation generation 69.7% 6.1% are observed. Metagenome analysis inhibition experiments indicate released by corrosive bacteria utilized Methanothrix accomplishing CO2-to-CH4 conversion potential intracellular extracellular transfer. The results activity tests four donors (i.e., ZVI, stainless steel, H2, acetate) suggest ZVI-dependent methanogenesis dominate overall compared with hydrogenotrophic acetoclastic methanogenesis, which provides new insight into autotrophic metabolism

Language: Английский

Citations

5