Elucidating Microbial Iron Corrosion Mechanisms with a Hydrogenase-Deficient Strain of Desulfovibrio vulgaris

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2024, Volume and Issue: unknown

Published: March 24, 2024

Abstract Sulfate-reducing microorganisms extensively contribute to the corrosion of ferrous metal infrastructure. There is substantial debate over their mechanisms. We investigated Fe 0 with Desulfovibrio vulgaris , sulfate reducer most often employed in studies. Cultures were grown both lactate and as potential electron donors replicate common environmental condition which organic substrates help fuel growth corrosive microbes. was corroded cultures a D. hydrogenase-deficient mutant 1:1 correspondence between loss H 2 accumulation expected for oxidation coupled + reduction . This result extent indicated that not capable direct -to-microbe transfer even though it provided supplementary energy source presence abundant sulfide. Corrosion greater than sterile controls, demonstrating removal necessary enhanced observed The parental -consuming strain more strain, could be attributed producing sulfide further stimulated oxidation. results suggest consumption microbially corrosion, but can indirectly promote by increasing generation from reduction. finding that, incapable uptake reaffirms metal-to-microbe has yet rigorously described sulfate-reducing Impact Statement economic impact microbial iron-containing metals substantial. A better understanding how microbes accelerate lead development methods prevent corrosion. presented here refute suggestion, frequently made microbiology literature, stimulates Also refuted, are previous claims directly extracting electrons consistent concept produced sulfate-reducers promotes production 2. illustrate appropriate mutants provide rigor mechanism

Language: Английский

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Durability and mass loss effect of heat-treated stainless-steel cathode for methane production from organic and inorganic carbon in a bioelectrochemical system

Journal of Hazardous Materials Advances, Journal Year: 2024, Volume and Issue: unknown, P. 100573 - 100573

Published: Dec. 1, 2024

Language: Английский

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The Nitrate-Dependent Impact of Carbon Source Starvation on EH40 Steel Corrosion Induced by the Coexistence of Desulfovibrio vulgaris and Pseudomonas aeruginosa

Metals, Journal Year: 2023, Volume and Issue: 13(2), P. 413 - 413

Published: Feb. 16, 2023

Carbon source starvation can promote steel corrosion in the presence of a pure culture through extracellular electron transfer (EET). However, impact carbon on induced by mixed strains is still unknown. This work investigated EH40 Desulfovibrio vulgaris and Pseudomonas aeruginosa, typical species sulfate- nitrate-reducing bacteria. It was found that depended nitrate addition. When (5 g∙L−1 NaNO3) not added, promoted starvation. initially starvation, but later inhibited with The behaviors different systems were closely related to numbers biofilms their metabolic activities, mechanisms revealed.

Language: Английский

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Microorganisms Involved in the Biodegradation and Microbiological Corrosion of Structural Materials

Coatings, Journal Year: 2023, Volume and Issue: 13(10), P. 1683 - 1683

Published: Sept. 25, 2023

Microbiologically influenced corrosion (MIC) is the process of material degradation in presence microorganisms and their biofilms. This an environmentally assisted type corrosion, which highly complex challenging to fully understand. Different metallic materials, such as steel alloys, magnesium aluminium titanium have been reported adverse effects MIC on applications. Though many researchers bacteria primary culprit microbial several other microorganisms, including fungi, algae, archaea, lichen, found cause metal non-metal surfaces. However, less attention given caused by lichens. In this review paper, different bacteria, lichens, properties engineering materials discussed detail. aims summarize all corrosive that directly or indirectly structural materials. Accusing every case without a proper investigation site in-depth study biofilm secreted metabolites can create problems understanding real materials’ failure. To identify agent any environment, it important kinds exist specific environment.

Language: Английский

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Electrobiocorrosion by Microbes without Outer-Surface Cytochromes

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2023, Volume and Issue: unknown

Published: July 26, 2023

Abstract Anaerobic microbial corrosion of iron-containing metals causes extensive economic damage. Some microbes are capable direct metal-to-microbe electron transfer (electrobiocorrosion), but the prevalence electrobiocorrosion among diverse methanogens and acetogens is poorly understood because a lack tools for their genetic manipulation. Previous studies have suggested that respiration with 316L stainless steel as donor indicative because, unlike pure Fe 0 , does not abiotically generate H 2 an intermediary carrier. Here we report all ( Methanosarcina vacuolata Methanothrix soehngenii Methanobacterium strain IM1) Sporomusa ovata Clostridium ljungdahlii ) evaluated respired donor, only M. Mx S. were electrobiocorrosion. The electrobiocorrosive required acetate additional energy source in order to produce methane from steel. Co-cultures Mx. demonstrated how can provide during corrosion. Not was IM1 electrobiocorrosion, it also did accept electrons Geobacter metallireducens effective electron- donating partner interspecies directly . finding despite outer-surface c -type cytochromes previously found be important other microbes, demonstrates there multiple strategies making electrical contact Impact Statement Understanding anaerobic receive likely lead novel mitigating metals, which has enormous impact. Electrobiocorrosion, relatively recently recognized mechanism. It cultures when oxidation inhibited by deletion genes known involved forms extracellular exchange. However, many obvious connections difficult genetically manipulate. study reported here provides alternative approach evaluating whether require results indicate IM1, electrobiocorrosive, contrast previous speculation. some without do appear suggesting this mechanism may more widespread than thought.

Language: Английский

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