O-GlcNAcylation dictates pyroptosis DOI Creative Commons
Yue Lang, Jincheng Li, Leiliang Zhang

et al.

Frontiers in Immunology, Journal Year: 2024, Volume and Issue: 15

Published: Dec. 17, 2024

O-GlcNAcylation is a dynamic post-translational modification involving the attachment of N-acetylglucosamine to serine and threonine residues. This review emphasizes its role in regulating signaling pathways pyroptosis. Specifically, GSDMD linked modulation pyroptosis, suggesting that enhancing could be crucial for improving hypoperfusion sepsis. Additionally, GSDME, another member gasdermin family, facilitates macrophage pyroptosis through induced by high glucose levels context periodontitis. The also examines effects on NLRP3 inflammasome regulators, including NEK7 NF-κB. Overall, this pathogenesis conditions such as sepsis, periodontitis, osteoarthritis, identifying potential therapeutic targets managing inflammatory responses targeted modulation.

Language: Английский

Cellular SLC35B4 promotes internalization during influenza A virus entry DOI Creative Commons
Guangwen Wang, Li Jiang,

Yan Ya

et al.

mBio, Journal Year: 2025, Volume and Issue: unknown

Published: March 25, 2025

ABSTRACT SLC35B4, a nucleotide sugar transporter that mediates the transport of UDP-GlcNAc and UDP-xylose, was found to be required for replication influenza A virus (IAV) H5N1 subtype in our genome-wide siRNA library screen. We defective IAV SLC35B4-deficient A549 cells independent strain specificity, virulence Slc35b4 knockdown mice also decreased. By examining individual stages cycle, we discovered amount internalized significantly reduced SLC35B4-knockout cells. Mechanistically, SLC35B4 facilitated by transporting which attaches serine residue heparan sulfate proteoglycans (HSPGs) (HS) biosynthesis pathway. Knockdown associated host factors (i.e., XYLT2, B4GALT7, EXT1, EXT2) HS pathway impaired replication. Furthermore, revealed AGRN, unique HSPG family member, important endocytosis Moreover, homeostasis AGRN protein regulated modification mediated initial UDP-xylose thereby affecting expression level endocytic adapter AP2B1 influence internalization. Collectively, these findings establish is an regulator uncover underlying mechanisms employs activity promote IMPORTANCE The entry process represents favorable target drug development. In this study, identified as factor efficient different subtypes vitro mice. employed its pathway, assisting internalization into early stage viral infection. Consistently, several downstream i.e., EXT2, well specific member were IAV. UDP-xylose-transporting involved regulation modification, influenced levels AP2B1. Together, identification SLC35B4–XYLT2–B4GALT7–EXT1–EXT2–AGRN–AP2B1 axis may shed light on development potential anti-IAV therapeutics.

Language: Английский

Citations

1
Concanavalin A-assisted extraction-free one-pot RPA-CRISPR/Cas12a assay for rapid detection of HPV16 DOI

You Nie,

Xiaohui Li, Yang Wen

et al.

Research Square (Research Square), Journal Year: 2025, Volume and Issue: unknown

Published: April 18, 2025

Abstract Human papillomavirus (HPV) infection is a major threat to women’s health worldwide. High-risk subtypes, particularly HPV16, require rigorous screening and long-term surveillance control cervical cancer. However, traditional HPV testing hampered by the need for nucleic acid extraction, reliance on specialized technicians, fluorescence detection equipment, limiting its suitability rapid on-site testing. In this study, we developed Concanavalin A-assisted extraction-free one-pot recombinase polymerase amplification (RPA) CRISPR/Cas12a assay (ConRCA) HPV16. A-coated magnetic beads were used target enrichment nucleic-acid-extraction-free processing. Suboptimal protospacer-adjacent motifs achieve RPA–CRISPR/Cas12a assay. The ConRCA can be completed in approximately 25 min under isothermal conditions detect at least 1.2 copies/µL of HPV16 genomic DNA using reader or test strip. feasibility method was evaluated with 31 unextracted clinical samples. Compared qPCR, overall sensitivity 95% (19/20), specificity 100% (11/11). Our results indicate that has great potential utility as point-of-care identification HPV.

Language: Английский

Citations

0
All-atom virus simulations to tackle airborne disease DOI Creative Commons
Abigail C. Dommer, Nicholas Wauer, ‪Siewert J. Marrink

et al.

Current Opinion in Structural Biology, Journal Year: 2025, Volume and Issue: 92, P. 103048 - 103048

Published: May 3, 2025

We briefly review the latest computational studies focused on modeling viruses with classical all-atom (AA) molecular dynamics. report challenges, current solutions, and ongoing developments in constructing simulating whole viruses, discuss unique insights derived from AA mesoscale simulations that cannot be achieved by other means. Finally, we present new opportunities virology to understand viral aerostability within context of respiratory disease transmission. Overall, highlight value large-scale simulation champion need for increased interdisciplinary collaboration generate novel guide future research disease.

Language: Английский

Citations

0
O-GlcNAcylation dictates pyroptosis DOI Creative Commons
Yue Lang, Jincheng Li, Leiliang Zhang

et al.

Frontiers in Immunology, Journal Year: 2024, Volume and Issue: 15

Published: Dec. 17, 2024

O-GlcNAcylation is a dynamic post-translational modification involving the attachment of N-acetylglucosamine to serine and threonine residues. This review emphasizes its role in regulating signaling pathways pyroptosis. Specifically, GSDMD linked modulation pyroptosis, suggesting that enhancing could be crucial for improving hypoperfusion sepsis. Additionally, GSDME, another member gasdermin family, facilitates macrophage pyroptosis through induced by high glucose levels context periodontitis. The also examines effects on NLRP3 inflammasome regulators, including NEK7 NF-κB. Overall, this pathogenesis conditions such as sepsis, periodontitis, osteoarthritis, identifying potential therapeutic targets managing inflammatory responses targeted modulation.

Language: Английский

Citations

0