Critical Factors in Sample Collection and Preparation for Clinical Metabolomics of Underexplored Biological Specimens
Metabolites,
Journal Year:
2024,
Volume and Issue:
14(1), P. 36 - 36
Published: Jan. 5, 2024
This
review
article
compiles
critical
pre-analytical
factors
for
sample
collection
and
extraction
of
eight
uncommon
or
underexplored
biological
specimens
(human
breast
milk,
ocular
fluids,
sebum,
seminal
plasma,
sweat,
hair,
saliva,
cerebrospinal
fluid)
under
the
perspective
clinical
metabolomics.
These
samples
are
interesting
metabolomics
studies
as
they
reflect
status
living
organisms
can
be
applied
diagnostic
purposes
biomarker
discovery.
Pre-collection
procedures
critical,
requiring
protocols
to
standardized
avoid
contamination
bias.
Such
must
consider
cleaning
area,
stimulation,
diet,
food
drug
intake,
among
other
that
impact
lack
homogeneity
group.
Precipitation
proteins
removal
salts
cell
debris
most
used
preparation
procedures.
intends
provide
a
global
view
practical
aspects
results,
serving
starting
point
designing
metabolomic
experiments.
Language: Английский
Colostrum Lactoferrin Following Active and Recovered SARS-CoV-2 Infections during Pregnancy
Paulina Gaweł,
No information about this author
Błażej Łukianowski,
No information about this author
Katarzyna Kościelska−Kasprzak
No information about this author
et al.
Biomedicines,
Journal Year:
2024,
Volume and Issue:
12(5), P. 1120 - 1120
Published: May 17, 2024
Lactoferrin
(Lf),
which
is
particularly
abundant
in
human
breast
milk
during
the
early
stages
of
lactation,
provides
protection
against
a
variety
infections,
including
viral
and
has
demonstrated
activity
severe
acute
respiratory
syndrome
coronavirus
2
(SARS-CoV-2).
The
objective
this
study
was
to
measure
concentrations
Lf
colostrum
mothers
with
active
disease
2019
(COVID-19)
infections
delivery,
history
COVID-19
pregnancy,
non-infected
controls.
In
cross-sectional
study,
samples
from
41
lactating
confirmed
SARS-CoV-2
infection
(asymptomatic
or
symptomatic)
(both
past
infections)
were
collected.
Twenty-eight
collected
pre-pandemic
period
served
as
control
group.
An
enzyme-linked
immunosorbent
assay
performed
analyze
concentrations.
Concentrations
closely
related
virus
infection.
Colostrum
contained
higher
lactoferrin
compared
highest
found
delivery
when
post-infection
samples.
This
observed
increase
suggests
that
it
may
be
an
important
protective
factor
for
breastfed
infants,
finding
relevant
pandemic
remains
whenever
breastfeeding
mother
infected.
Language: Английский
Ultrasound-assisted three-phase hollow fiber microextraction-based method for untargeted metabolomics
M.C. Villegas-Álvarez,
No information about this author
Ana Arias‐Borrego,
No information about this author
Inés Velasco
No information about this author
et al.
Microchemical Journal,
Journal Year:
2023,
Volume and Issue:
195, P. 109473 - 109473
Published: Oct. 7, 2023
Language: Английский
Untargeted metabolomic analysis of human milk from healthy mothers reveals drivers of metabolite variability
Scientific Reports,
Journal Year:
2024,
Volume and Issue:
14(1)
Published: Sept. 6, 2024
Language: Английский
Metallomic and Untargeted Metabolomic Signatures of Human Milk from SARS‐CoV‐2 Positive Mothers
Molecular Nutrition & Food Research,
Journal Year:
2022,
Volume and Issue:
66(16)
Published: June 10, 2022
Lack
of
information
about
the
impact
maternal
severe
acute
respiratory
syndrome
coronavirus
2
(SARS-CoV-2)
infection
on
elemental
and
metabolomic
profile
human
milk
(HM).An
observational
study
HM
from
mothers
with
COVID-19
is
conducted
including
a
prepandemic
control
group.
Maternal-infant
clinical
records
symptomatology
are
recorded.
The
absolute
quantification
elements
untargeted
relative
profiles
determined
by
inductively
coupled
plasma
mass
spectrometry
gas
chromatography
to
spectrometry,
respectively.
Associations
SARS-CoV-2
antibodies
studied.
has
significant
composition.
reduces
concentrations
Fe,
Cu,
Se,
Ni,
V,
Aluminium
(Al)
increases
Zn
compared
samples.
A
total
18
individual
metabolites
amino
acids,
peptides,
fatty
acids
conjugates,
purines
derivatives,
alcohols,
polyols
significantly
different
in
positive
mothers.
Aminoacyl-tRNA
biosynthesis,
phenylalanine,
tyrosine
tryptophan
linoleic
acid
pathways
altered.
Differences
obtained
depending
symptomatic
asymptomatic
status.This
provides
unique
insights
that
warrants
further
research
due
potential
implications
for
infant
health.
Language: Английский
COVID-19 and Pregnancy: A Dangerous Mix for Bone Turnover and Metabolism Biomarkers in Placenta and Colostrum
Journal of Clinical Medicine,
Journal Year:
2024,
Volume and Issue:
13(7), P. 2124 - 2124
Published: April 6, 2024
Background:
In
pregnant
women,
COVID-19
can
alter
the
metabolic
environment,
cell
metabolism,
and
oxygen
supply
of
trophoblastic
cells
and,
therefore,
have
a
negative
influence
on
essential
mechanisms
fetal
development.
The
purpose
this
study
was
to
investigate,
for
first
time,
effects
infection
during
pregnancy
with
regard
bone
turnover
endocrine
function
several
biomarkers
in
colostrum
placenta.
Methods:
One
hundred
twenty-four
mothers
were
recruited
from
three
hospitals
between
June
2020
August
2021
assigned
two
groups:
Control
group
group.
Metabolism
addressed
placental
tissue
colostrum.
Results:
Lipocalin-2
resistin
levels
higher
placenta,
revealing
an
underlying
pro-inflammatory
status
gestation
period
suffering
COVID-19;
decrease
GLP-1
leptin
also
observed
As
adiponectin,
resistin,
insulin,
their
concentrations
showed
increase;
GLP-1,
leptin,
PYY
reported
compared
control
Conclusions:
turnover,
samples
lower
OPG,
while
DKK-1
increased
Colostrum
SOST,
PTH
group,
fact
that
could
noteworthy
implications
energy
skeletal
development,
postnatal
density
mineralization.
Further
research
is
needed
explain
pathogenic
mechanism
may
affect
pregnancy,
so
as
assess
short-term
long-term
outcomes
infants’
health.
Language: Английский
Associations between SARS-CoV-2 Infection or COVID-19 Vaccination and Human Milk Composition: A Multi-Omics Approach
bioRxiv (Cold Spring Harbor Laboratory),
Journal Year:
2024,
Volume and Issue:
unknown
Published: April 15, 2024
ABSTRACT
The
risk
of
contracting
SARS-CoV-2
via
human
milk-feeding
is
virtually
non-existent.
Adverse
effects
COVID-19
vaccination
for
lactating
individuals
are
not
different
from
the
general
population,
and
no
evidence
has
been
found
that
their
infants
exhibit
adverse
effects.
Yet,
there
remains
substantial
hesitation
among
this
population
globally
regarding
safety
these
vaccines.
Herein
we
aimed
to
determine
if
compositional
changes
in
milk
occur
following
infection
or
vaccination,
including
any
vaccine
components.
Using
an
extensive
multi-omics
approach,
compared
unvaccinated
was
associated
with
significant
differences
67
proteins,
385
lipids,
13
metabolites.
In
contrast,
lipids
metabolites,
although
it
fewer
proteins.
Compositional
differed
by
vaccine.
Changes
were
greatest
after
1-6
hours
mRNA-based
Moderna
(8
changed
proteins),
3
days
Pfizer
(4
adenovirus-based
Johnson
(13
proteins)
Proteins
both
natural
mainly
systemic
inflammatory
responses.
addition,
components
detected
sample.
Together,
our
data
provide
only
minimal
composition
due
much
greater
infection.
IMPORTANCE
impact
observed
global
on
infant
health
remain
unknown.
These
findings
emphasize
importance
vaccinating
against
COVID-19,
as
be
far
less
evident
Importantly,
vaccination.
Language: Английский
Associations between SARS-CoV-2 Infection or COVID-19 Vaccination and Human Milk Composition: A Multi-Omics Approach
Journal of Nutrition,
Journal Year:
2024,
Volume and Issue:
154(12), P. 3566 - 3574
Published: Oct. 11, 2024
Language: Английский
Human Milk Metals and Metalloids Shape Infant Microbiota
Food & Function,
Journal Year:
2024,
Volume and Issue:
unknown
Published: Jan. 1, 2024
Human
milk
metals
and
metalloids
are
variable
significantly
correlate
with
infant
gut
microbiota
abundance
alpha
diversity
indices.
Language: Английский