Synthesis, Physical and Anti-Inflammatory Properties of Stigmasteryl Vanillate, Stigmasteryl Protocatechuate and Stigmasteryl Sinapate DOI

Xuan Yang,

Xinyue Zou, Ting Xu

et al.

Published: Jan. 1, 2023

As a class of phytosterols, stigmasterols (STIs) and their derivatives are important food additives intermediates, which confer substantial benefits to human health. Here, three novel stigmasteryl phenolates, namely vanillate (VAN), protocatechuate (PRO) sinapate (SIN) were synthesized through the Steglich reaction. In addition, stability was evaluated by differential scanning calorimetry thermogravimetric analysis. HPLC analysis revealed an enhancement in water solubility after esterification with phenolic acid. vitro digestion model, bioaccessibility phenolates significantly higher than that STI. Regarding anti-inflammatory properties, VAN, PRO, SIN exhibit superior effects against TNF-α induced pro-inflammatory responses HK-2 cells compared These results indicate possess improved digestibility terms physical properties STI, as well enhanced functionally.

Language: Английский

Fisetin Delays Postovulatory Oocyte Aging by Regulating Oxidative Stress and Mitochondrial Function through Sirt1 Pathway DOI Creative Commons
Xupeng Xing,

Yalin Liang,

Yanan Li

et al.

Molecules, Journal Year: 2023, Volume and Issue: 28(14), P. 5533 - 5533

Published: July 20, 2023

The quality of oocytes determines the development potential an embryo and is dependent on their timely fertilization after ovulation. Postovulatory oocyte aging inevitable factor during some assisted reproduction technology procedures, which results in poor rates impairs development. We found that fisetin, a bioactive flavonol contained fruits vegetables, delayed postovulatory mice. Fisetin improved aged inhibited Sirt1 reduction oocytes. increased GSH level Sod2 transcription to inhibit ROS accumulation Meanwhile, fisetin attenuated aging-induced spindle abnormalities, mitochondrial dysfunction, apoptosis. At molecular level, decreased aberrant expression H3K9me3. In addition, levels Tfam genes Co2 Atp8 by upregulating Finally, inhibition reversed anti-aging effects fisetin. Taken together, Sirt1.

Language: Английский

Citations

15
Updating the Role of JUNO and Factors Involved in Its Function during Fertilization DOI

Lucía Díaz-Fuster,

Paula Sáez‐Espinosa, Isabel Moya

et al.

Cells Tissues Organs, Journal Year: 2025, Volume and Issue: unknown, P. 1 - 16

Published: April 1, 2025

Introduction: The final step of the fertilization process involves gametes adhesion and fusion. JUNO is an essential folate receptor 4 protein present in ooplasm oocytes, which binds to IZUMO1, its on sperm surface. Both proteins are indispensable for sperm-oocyte interaction, their absence results infertility. Despite importance reproduction, there still controversy about how different factors affect functionality JUNO. Therefore, goal this study was provide a comprehensive overview what we know so far presence Methods: In order accomplish this, total 198 articles were identified. Based both inclusion exclusion criteria, 40 finally included study. Results: showed that during oocyte maturation, expression levels undergo alterations and, some instances, cross-species gamete fusion possible. Additionally, it has been observed exposure oocytes such as bisphenol A, 17α-ethynylestradiol, diazinon, benzo(a)pyrene, butylparaben, bis(2-ethylhexyl) phthalate, hydroxyurea, dichlorophenol, isoniazid, para-phenylenediamine disrupt decrease rates. Moreover, ionic radiation, vitrification, synthetic materials microplastics same effect. Nonetheless, other compounds melatonin, mogroside V, cholesterol-loaded methyl-β-cyclodextrin, protocatechuic acid, coenzyme Q10, resveratrol, Shoutai pills have shown enhance female fertility terms functionality. Conclusion: summary, update highlights crucial role reveals experimental procedures activity.

Language: Английский

Citations

0
Phytosterol organic acid esters: Characterization, anti-inflammatory properties and a delivery strategy to improve mitochondrial function DOI Creative Commons
Xinyue Zou, Ting Xu,

Tian Zhao

et al.

Current Research in Food Science, Journal Year: 2024, Volume and Issue: 8, P. 100702 - 100702

Published: Jan. 1, 2024

Phytosterol organic acid esters are important food resources and the components of biomembrane structure. Due to lack extraction synthesis techniques, more research has been focused on phytosterols, phytosterol have encountered a bottleneck, but confer substantial benefits human health. In this study, stigmasteryl vanillate (VAN), protocatechuate (PRO) sinapate (SIN) were prepared through Steglich reaction. The processes promotable products reach up 95% purity. addition, their stability was evaluated by differential scanning calorimetry thermogravimetric analysis. HPLC analysis revealed an enhancement in water solubility after esterification with phenolic acid. vitro digestion model, bioaccessibility phenolates significantly higher than that stigmasterols (STIs). Regarding anti-inflammatory properties, VAN, PRO, SIN exhibit superior effects against TNF-α induced pro-inflammatory responses compared STI. All supplementation increased ATP production, basal, maximal oxygen consumption rate mitochondrial stress test. Overall, we present method for phenolates. It will contribute development ester analysis, functions utilization food. Moreover, nutrient-stigmasterol hybrids tactic constructed is practical can become targeted delivery strategy enhanced effects.

Language: Английский

Citations

3
A non-antibiotic antimicrobial drug, a biological bacteriostatic agent, is useful for treating aerobic vaginitis, bacterial vaginosis, and vulvovaginal candidiasis DOI Creative Commons
Zhen Zeng,

Pei Li,

Jiayi Lu

et al.

Frontiers in Microbiology, Journal Year: 2024, Volume and Issue: 15

Published: May 27, 2024

Background Vaginitis is a common infection in women, with approximately 75% of women experiencing at least one episode during their lifetime. Although antimicrobial agents are widely used to treat vaginitis, recurrent vaginitis occurs some patients. Resistance these the major cause vaginitis. Therefore, there an urgent need develop novel drugs. Methods We investigated efficacy new biological bacteriostatic agent (BBA), composed lysozyme, phytoalexin, chitosan oligosaccharide, sinensetin, 18β/20α-glycyrrhizin, and betaine, against using vitro vivo studies. First, we evaluated antibacterial effects BBA 13 microbial strains commonly present aerobic bacterial vaginosis, vulvovaginal candidiasis, healthy vaginas. Second, assessed safety various doses administered orally for 4 weeks female mice. Third, examined anti-proliferative anti-inflammatory Candida albicans- , glabrata -, Gardnerella -induced models. Finally, anti-vaginitis effect gel prepared 0.5% (w/v) ammonium acryloyldimethyltaurate/Vp copolymer. Results effectively suppressed growth main causative pathogens . BBA, either undiluted or diluted two-fold, inhibited all microorganisms cultured 8 h. No obvious organ damage was detected when Both alone 70% formulation proliferation C. albicans vaginal lavage samples alleviated tissue inflammation mice The performed better than treating infected vaginalis Conclusion caused by G.

Language: Английский

Citations

3
Dithiothreitol prevents the spontaneous release of cortical granules in in vitro aged mouse oocytes by protecting regulatory proteins of cortical granules exocytosis and thickening the cortical actin cytoskeleton DOI
Paula Wetten,

Omar Guillermo Klinsky,

Marcela A. Michaut

et al.

Theriogenology, Journal Year: 2024, Volume and Issue: 229, P. 53 - 65

Published: Aug. 14, 2024

Language: Английский

Citations

0
Phytochemical, Pharmacological and Medicinal Properties of Carapa procera, Trichilia monadelpha, Spathodea campanulata and Gymnosporia senegalensis DOI Creative Commons
Ran Zhou, Aboagye Kwarteng Dofuor,

Peter Atta-Adjei

et al.

Natural Product Communications, Journal Year: 2024, Volume and Issue: 19(11)

Published: Nov. 1, 2024

Carapa procera DC ( C. procera), Trichilia monadelpha (Thonn.) J.J.de Wilde T. monadelpha), Spathodea campanulata P.Beauv S. campanulata) and Gymnosporia senegalensis Loes (G. senegalensis) are medicinal plants which used folklorically in Ghana, West Africa, for the treatment management of diseases. However, there is little scientific evidence documentation regarding their ethnobotanical uses, phytochemical constituents pharmacology activities. The objective this study was to provide a comprehensive literature synthesis composition pharmacological significance procera, G. senegalensis, future research health benefits. Databases utilized search included PubMed, Web Science, Scopus, Google Scholar, PubChem DrugBank. All plant names were checked with “World Flora Online” www.worldfloraonline.org ). Pharmacological studies conducted majority cases indicated anti-inflammatory, anti-oxidant or antimicrobial activities monadelpha, senegalensis. Phytochemical analysis also revealed botanical that may potentially be involved observed properties. This review will go long way promote efficacious use these plants. much remains done respect preclinical clinical investigations order harness full potential.

Language: Английский

Citations

0
Synthesis, Physical and Anti-Inflammatory Properties of Stigmasteryl Vanillate, Stigmasteryl Protocatechuate and Stigmasteryl Sinapate DOI

Xuan Yang,

Xinyue Zou, Ting Xu

et al.

Published: Jan. 1, 2023

As a class of phytosterols, stigmasterols (STIs) and their derivatives are important food additives intermediates, which confer substantial benefits to human health. Here, three novel stigmasteryl phenolates, namely vanillate (VAN), protocatechuate (PRO) sinapate (SIN) were synthesized through the Steglich reaction. In addition, stability was evaluated by differential scanning calorimetry thermogravimetric analysis. HPLC analysis revealed an enhancement in water solubility after esterification with phenolic acid. vitro digestion model, bioaccessibility phenolates significantly higher than that STI. Regarding anti-inflammatory properties, VAN, PRO, SIN exhibit superior effects against TNF-α induced pro-inflammatory responses HK-2 cells compared These results indicate possess improved digestibility terms physical properties STI, as well enhanced functionally.

Language: Английский

Citations

0