Deciphering probiotic potential: a comprehensive guide to probiogenomic analyses DOI
Yasmin Neves Vieira Sabino, Aline Dias Paiva,

Bárbara Ribeiro Fonseca

et al.

Future Microbiology, Journal Year: 2025, Volume and Issue: unknown, P. 1 - 12

Published: April 14, 2025

In recent years, the study of probiotics has advanced significantly, driven by growing interest in their potential health benefits and applications food pharmaceutical industries. Probiotics are claimed to enhance gut health, modulate immune responses, improve digestion, synthesize beneficial compounds for host, even impact mental through gut-brain axis. However, traditional vitro methods identifying have limitations, such as low reproducibility phenotypic screening, limited capacity discover new strains, restricted evaluation safety, inefficiencies fully understanding biological properties responsible health-promoting effects. Advancements genomic analysis technology provided a cost-effective approach further explore probiotic strains molecular mechanisms driving effects hosts. Here, we describe comprehensive workflow probiogenomic aimed at establishing gold-standard pipeline screening based on genome sequencing. This encompasses steps from acquiring genomes safety-related features, plasticity, markers whole-genome addition, this outlines respective methodological approaches provides most database documented date, comprising 243 genes potentially associated with function.

Language: Английский

Deciphering probiotic potential: a comprehensive guide to probiogenomic analyses DOI
Yasmin Neves Vieira Sabino, Aline Dias Paiva,

Bárbara Ribeiro Fonseca

et al.

Future Microbiology, Journal Year: 2025, Volume and Issue: unknown, P. 1 - 12

Published: April 14, 2025

In recent years, the study of probiotics has advanced significantly, driven by growing interest in their potential health benefits and applications food pharmaceutical industries. Probiotics are claimed to enhance gut health, modulate immune responses, improve digestion, synthesize beneficial compounds for host, even impact mental through gut-brain axis. However, traditional vitro methods identifying have limitations, such as low reproducibility phenotypic screening, limited capacity discover new strains, restricted evaluation safety, inefficiencies fully understanding biological properties responsible health-promoting effects. Advancements genomic analysis technology provided a cost-effective approach further explore probiotic strains molecular mechanisms driving effects hosts. Here, we describe comprehensive workflow probiogenomic aimed at establishing gold-standard pipeline screening based on genome sequencing. This encompasses steps from acquiring genomes safety-related features, plasticity, markers whole-genome addition, this outlines respective methodological approaches provides most database documented date, comprising 243 genes potentially associated with function.

Language: Английский

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