Microfluidic Production of Ultrathin, Handleable Collagen Sheets Exhibiting Toe‐heel Tensile Behavior DOI Creative Commons
Yuming Zhang, Shashi Malladi,

Bangan Wang

et al.

Advanced Materials Technologies, Journal Year: 2025, Volume and Issue: unknown

Published: May 1, 2025

Abstract The extracellular matrix (ECM) of cardiovascular tissues displays a non‐linear, strain‐dependent elastic modulus, attributed to the hierarchical organization collagen. At low loads, these exhibit compliance, permit contraction or dilation, while at high they stiffen and increase their mechanical strength least tenfold. Although collagen gels are widely used in 3D cell culture, tissue engineering, biofabrication, current engineering techniques fail replicate this microscale. As result, often lack either non‐linear tensile behavior physiologically relevant native tissues. To address limitation, we present ultrathin, templated sheets (1.8 microns thin 10 mm wide) from an acidic solution using microfluidic wet spinning process, incorporating later removing microscale oil droplets 2.3% volume concentration. Templated two‐fold fibril alignment dispersion compared with non‐templated ones. When assessed along length, Young's modulus increases 62‐fold 90% failure strain, recapitulating load‐bearing We anticipate that ultrathin will serve as substrate materials for bottom‐up fabrication biomaterials structures vitro applications implantation.

Language: Английский

Microfluidic Production of Ultrathin, Handleable Collagen Sheets Exhibiting Toe‐heel Tensile Behavior DOI Creative Commons
Yuming Zhang, Shashi Malladi,

Bangan Wang

et al.

Advanced Materials Technologies, Journal Year: 2025, Volume and Issue: unknown

Published: May 1, 2025

Abstract The extracellular matrix (ECM) of cardiovascular tissues displays a non‐linear, strain‐dependent elastic modulus, attributed to the hierarchical organization collagen. At low loads, these exhibit compliance, permit contraction or dilation, while at high they stiffen and increase their mechanical strength least tenfold. Although collagen gels are widely used in 3D cell culture, tissue engineering, biofabrication, current engineering techniques fail replicate this microscale. As result, often lack either non‐linear tensile behavior physiologically relevant native tissues. To address limitation, we present ultrathin, templated sheets (1.8 microns thin 10 mm wide) from an acidic solution using microfluidic wet spinning process, incorporating later removing microscale oil droplets 2.3% volume concentration. Templated two‐fold fibril alignment dispersion compared with non‐templated ones. When assessed along length, Young's modulus increases 62‐fold 90% failure strain, recapitulating load‐bearing We anticipate that ultrathin will serve as substrate materials for bottom‐up fabrication biomaterials structures vitro applications implantation.

Language: Английский

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