High-Resolution LC-MS Simultaneous Quantification of Forty-Six Compounds from Jatropha podagrica Fruit Recommends Four Top Antioxidant Contributors as Q-Markers DOI Creative Commons

Rongxin Cai,

Xican Li, Hongxia Liang

et al.

Molecules, Journal Year: 2025, Volume and Issue: 30(3), P. 722 - 722

Published: Feb. 5, 2025

There has been no chemical analysis of the fruit medicinal plant Jatropha podagrica until now. The current study aimed to qualitatively and quantitatively analyze J. using a high-resolution LC-MS strategy, i.e., library-comparison ultra-high-performance liquid chromatography-Quadrupole-Orbitrap-tandem mass spectrometry. strategy putatively identified 46 compounds from fresh fruit. During putative identification, 10 isomers (e.g., (vitexin vs. isovitexin) were completely distinguished each other. Thereafter, all simultaneously quantified authentic standard comparison method. Finally, they also subjected 2,2′-azino bis (3-ethylbenzothiazolin-6-sulfonic acid radical (ABTS+•)-scavenging assay characterize their relative antioxidant capacities. Their capacities thus multiplied by contents calculate contribution values, respectively. Corilagin, gallic acid, ellagic phillygenin exhibited highest percentages thereby suggested as four top contributors. are recommended build up quality-markers (Q-markers) system fruits. All these findings can help develop fruits potential resource natural medicine.

Language: Английский

High-Resolution LC-MS Simultaneous Quantification of Forty-Six Compounds from Jatropha podagrica Fruit Recommends Four Top Antioxidant Contributors as Q-Markers DOI Creative Commons

Rongxin Cai,

Xican Li, Hongxia Liang

et al.

Molecules, Journal Year: 2025, Volume and Issue: 30(3), P. 722 - 722

Published: Feb. 5, 2025

There has been no chemical analysis of the fruit medicinal plant Jatropha podagrica until now. The current study aimed to qualitatively and quantitatively analyze J. using a high-resolution LC-MS strategy, i.e., library-comparison ultra-high-performance liquid chromatography-Quadrupole-Orbitrap-tandem mass spectrometry. strategy putatively identified 46 compounds from fresh fruit. During putative identification, 10 isomers (e.g., (vitexin vs. isovitexin) were completely distinguished each other. Thereafter, all simultaneously quantified authentic standard comparison method. Finally, they also subjected 2,2′-azino bis (3-ethylbenzothiazolin-6-sulfonic acid radical (ABTS+•)-scavenging assay characterize their relative antioxidant capacities. Their capacities thus multiplied by contents calculate contribution values, respectively. Corilagin, gallic acid, ellagic phillygenin exhibited highest percentages thereby suggested as four top contributors. are recommended build up quality-markers (Q-markers) system fruits. All these findings can help develop fruits potential resource natural medicine.

Language: Английский

Citations

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