Developmental Biology, Journal Year: 2024, Volume and Issue: unknown
Published: Dec. 1, 2024
Language: Английский
ACS Pharmacology & Translational Science, Journal Year: 2024, Volume and Issue: 7(4), P. 967 - 990
Published: March 19, 2024
Precision medicine is transforming colorectal cancer treatment through the integration of advanced technologies and biomarkers, enhancing personalized effective disease management. Identification key driver mutations molecular profiling have deepened our comprehension genetic alterations in cancer, facilitating targeted therapy immunotherapy selection. Biomarkers such as microsatellite instability (MSI) DNA mismatch repair deficiency (dMMR) guide decisions, opening avenues for immunotherapy. Emerging liquid biopsies, artificial intelligence, machine learning promise to revolutionize early detection, monitoring, selection precision medicine. Despite these advancements, ethical regulatory challenges, including equitable access data privacy, emphasize importance responsible implementation. The dynamic nature with its tumor heterogeneity clonal evolution, underscores necessity adaptive strategies. future lies potential enhance patient care, clinical outcomes, understanding this intricate disease, marked by ongoing evolution field. current reviews focus on providing in-depth knowledge various diverse approaches utilized against at both biochemical levels.
Language: Английский
Citations
17
Frontiers in Pharmacology, Journal Year: 2025, Volume and Issue: 15
Published: Jan. 7, 2025
The epithelial-mesenchymal transition (EMT) is a biological process in which epithelial cells change into mesenchymal with fibroblast-like characteristics. EMT plays crucial role the progression of fibrosis. Classical inducers associated maintenance EMT, such as TGF-β1, have become targets several anti-EMT therapeutic strategies. Natural products from pentacyclic triterpene class emerged promising elements inhibiting EMT. Uvaol found olive trees (Olea europaea L.) known for its anti-inflammatory, antioxidant, and antiproliferative properties. Yet, effect on TGF-β1-induced alveolar unknown. present study aimed to investigate impact uvaol upon cultured A549 human cell line, classic vitro model studies Changes shape were measured using phase-contrast confocal microscopy, whereas protein expression levels immunofluorescence, flow cytometry, Western blotting. We also performed wound scratch experiments explore effects migration. had no significant cytotoxic cells. By contrast, changes morphology consistent largely suppressed by treatment uvaol. In addition, increased contents markers, namely, vimentin, N-cadherin, fibronectin cells, downregulated treatment. Furthermore, migration was significantly Mechanistically, prevented nuclear translocation β-catenin reduced ZEB1 These results provide compelling evidence that inhibits regulating proteins related profile likely modulating levels.
Language: Английский
Citations
0
Translational Oncology, Journal Year: 2024, Volume and Issue: 41, P. 101860 - 101860
Published: Jan. 22, 2024
Nutrient restriction in cancer cells can activate a number of stress response pathways for cell survival. We aimed to determine mechanistically how nutrient depletion colorectal (CRC) leads cellular adaptation. Cell survival under (ND) was evaluated by colony formation and vivo tumor assays. Lysosomes are activated with ND; therefore, we incubated the ND V-ATPase inhibitor Bafilomycin A1 (ND+Baf). The expression epithelial mesenchymal markers ND+Baf determined RNA sequencing RT-qPCR while motility an Chorioallantoic membrane (CAM) assay. Reorganization cytoskeletal network lysosomal positioning immunocytochemistry. 4 different lines showed high viability, forming ability increased one or more markers, suggesting activation partial (p)-EMT. observed further increase p-EMT numerous protrusions, decreased cell-cell adhesion 3D, cells. protrusions were primarily mediated microtubules enabled relocalization lysosomes from perinuclear region periphery. CRC cells, which exacerbated alkalinization. also containing lysosomes, may lead exocytosis enhanced motility.
Language: Английский
Citations
3
Frontiers in Oncology, Journal Year: 2025, Volume and Issue: 15
Published: Feb. 7, 2025
This review summarizes the applications and research progress of organoid models in colorectal cancer research. First, high incidence mortality rates are introduced, emphasizing importance organoids as a model. Second, this provides detailed introduction to concept, biological properties, organoids, including their strengths mimicking structural functional aspects organs. article further analyzes adult stem cell-derived pluripotent discusses advancements for basic research, drug development, personalized treatment evaluation prediction, regenerative medicine. Finally, prospects applying technology its significant value improving patient survival rates. In conclusion, systematically explains highlighting tremendous potential promising
Language: Английский
Citations
0
Traffic, Journal Year: 2024, Volume and Issue: 25(9)
Published: Sept. 1, 2024
ABSTRACT Limited nutrient availability in the tumor microenvironment can cause rewiring of signaling and metabolic networks to confer cancer cells with survival advantages. We show here that limitation glucose, glutamine serum from culture medium resulted a population high viability capacity form tumors vivo. These also displayed remarkable increase abundance size lysosomes. Moreover, lysosomes were located mainly perinuclear region nutrient‐limited cells; this translocation was mediated by rapid post‐transcriptional key endolysosomal trafficking protein Rab7a. The acidic could trap weakly basic drugs such as doxorubicin, mediating resistance drug, which be partially reversed lysosomal inhibitor bafilomycin A1. An vivo chorioallantoic membrane (CAM) assay indicated decrease microtumor volume when treated 5‐Fluorouracil (5‐FU) A1 compared either agent alone. Overall, our data indicate activation complementary pathways enable survive, proliferate acquire drug resistance.
Language: Английский
Citations
1
Biochimica et Biophysica Acta (BBA) - Molecular Basis of Disease, Journal Year: 2024, Volume and Issue: 1871(1), P. 167538 - 167538
Published: Oct. 9, 2024
Tissue transglutaminase 2 (TGM2) and matrix metalloproteinase 7 (MMP7) are suggested to be involved in cancer development progression, however, their specific role colon remains elusive. The present study investigated whether TGM2 MMP7 influence epithelial-mesenchymal-transition (EMT) processes of cells. was either overexpressed or knocked down SW480 HCT-116 cells, expression activity analyzed. Conversely, silenced its correlation with examined. Co-immunoprecipitation served evaluate TGM2-MMP7-interaction. were correlated invasion, migration, EMT marker (E-cadherin, N-cadherin, Slug, Snail), ERK/MEK signaling. overexpression enhanced activity, promoted cell migration EMT, characterized by increased N-cadherin Snail/Slug expression. knockdown resulted the opposite effects. Knocking associated reduced protein expression, invasion migration. Down-regulation diminished signaling, whereas up-regulation activated this pathway. ERK-inhibitor GDC-0994 blocked phosphorylation MEK/ERK suppressed MMP7. communicates cells forces signaling pathway triggers EMT. Inhibiting could thus offer new therapeutic options treat patients cancer, particularly prevent metastatic progression.
Language: Английский
Citations
1
Methods in molecular biology, Journal Year: 2024, Volume and Issue: unknown
Published: Jan. 1, 2024
Language: Английский
Citations
0
The Journal of Pathology Clinical Research, Journal Year: 2024, Volume and Issue: 10(6)
Published: Oct. 2, 2024
Abstract EGFR mutations are a major prognostic factor in lung adenocarcinoma. However, current detection methods require sufficient samples and costly. Deep learning is promising for mutation prediction histopathological image analysis but has limitations that it does not sufficiently reflect tumor heterogeneity lacks interpretability. In this study, we developed deep model to predict the presence of by analyzing patterns whole slide images (WSIs). We also introduced prevalence (EMP) score, which quantifies WSIs based on patch‐level predictions, evaluated its interpretability utility. Our estimates probability each patch partitioning WSI multiple‐instance predicts at level. utilized patch‐masking scheduler training strategy enable learn various EGFR. This study included 868 from adenocarcinoma patients collected three medical institutions: Hallym University Medical Center, Inha Hospital, Chungnam National Hospital. For test dataset, 197 were Ajou Center evaluate mutations. demonstrated performance with an area under receiver operating characteristic curve 0.7680 (0.7607–0.7720) precision‐recall 0.8391 (0.8326–0.8430). The EMP score showed Spearman correlation coefficients 0.4705 ( p = 0.0087) p.L858R 0.5918 0.0037) exon 19 deletions 64 subjected next‐generation sequencing analysis. Additionally, high scores associated papillary acinar 0.0038 0.0255, respectively), whereas low solid 0.0001). These results validate reliability our suggest can provide crucial information rapid screening treatment plans.
Language: Английский
Citations
0
Developmental Biology, Journal Year: 2024, Volume and Issue: unknown
Published: Dec. 1, 2024
Language: Английский
Citations
0