Cost-efficient analysis of cannabinoids in therapeutic oils using HPLC with UV and mass spectrometry detection DOI
Alexander Fries, Carlos Alberto Moldes, Laura S. Mazzaferro

et al.

Natural Product Research, Journal Year: 2024, Volume and Issue: unknown, P. 1 - 9

Published: Dec. 13, 2024

Cannabis oil, derived from

Language: Английский

Progress in the analysis of phytocannabinoids by HPLC and UPLC (or UHPLC) during 2020–2023 DOI

Lutfun Nahar,

Phanuphong Chaiwut, Sarita Sangthong

et al.

Phytochemical Analysis, Journal Year: 2024, Volume and Issue: 35(5), P. 927 - 989

Published: June 4, 2024

Organic molecules that bind to cannabinoid receptors are known as cannabinoids. These possess pharmacological properties similar those produced by Cannabis sativa L. High-performance liquid chromatography (HPLC) and ultra-performance (UPLC, also ultra-high-performance chromatography, UHPLC) have become the most widely used analytical tools for detection quantification of phytocannabinoids in various matrices. HPLC UPLC (or usually coupled an ultraviolet (UV), photodiode array (PDA), or mass spectrometric (MS) detector.

Language: Английский

Citations

4
Development of a rapid time-resolved fluorescence immunoassay for cannabidiol quantification in Cannabis sativa L DOI Creative Commons
Ziyan Fan,

Cai Xian-Jie,

Xiaojie Xu

et al.

Industrial Crops and Products, Journal Year: 2025, Volume and Issue: 226, P. 120626 - 120626

Published: Feb. 5, 2025

Language: Английский

Citations

0
A Systematic Study of Liquid Chromatography in Search of the Best Separation of Cannabinoids for Potency Testing of Hemp-Based Products DOI Creative Commons

Ayowole Owolabi,

Olalekan Ogunsola,

Emma Joens

et al.

Molecules, Journal Year: 2025, Volume and Issue: 30(4), P. 758 - 758

Published: Feb. 7, 2025

A study was conducted to search for the best separation of eighteen cannabinoids, maximum number cannabinoids that have been quantified so far, potency testing hemp-based products using liquid chromatography diode array detector (LC-DAD). The investigation utilized four column types, all sharing same dimension (150 mm × 2.1 mm) and core-shell particle size (2.7 µm), but different stationary phases: dimethyl-octadecyl (Poroshell 120 EC-C18), diisobutyl-octadecyl (Raptor ARC-18), reverse phase (RP)-carbamate (Cortecs Shield RP-18), RP-amide (Ascentis Express RP-Amide). resolution adjacent kept close 1.5 or higher, while time as short possible. fastest achieved within 15.0 min two sequential Raptor ARC-18 columns, with a mobile consisting 75.0% acetonitrile 25.0% aqueous solution 0.03% formic acid 0.5 mM ammonium formate at pH 2.97, flow rate mL/min. slightly improved obtained 18.5 Poroshell EC-C18 columns under similar conditions, except containing 77.5% reduced 0.45 mL/min due backpressure higher than 600 bars. Furthermore, rapid 7.0 by electrospray ionization tandem mass spectrometry (LC-ESI/MS/MS) Cortecs RP-18 column, 70.0% 30.0% 0.01% 1 3.38

Language: Английский

Citations

0
Thermodynamic and surface properties of 4-(Undecyloxy) benzoic acid thermotropic liquid crystal by inverse gas chromatography at infinite dilution DOI
Sabire Özcan, Sinem Taş, Birol Işık

et al.

Phase Transitions, Journal Year: 2025, Volume and Issue: unknown, P. 1 - 14

Published: Feb. 16, 2025

Language: Английский

Citations

0
Acid-Catalyzed Conversion of Cannabidiol to Tetrahydrocannabinols: En Route to Demystifying Manufacturing Processes and Controlling the Reaction Outcomes DOI

Alex Nivorozhkin,

Michael G. Palfreyman

Cannabis and Cannabinoid Research, Journal Year: 2025, Volume and Issue: unknown

Published: April 18, 2025

Background: Over the last decade, there has been a significant increase in production of multiple tetrahydrocannabidiol (THC) related products via acid catalysis cannabidiol (CBD). The widespread availability CBD and unregulated or poorly regulated nature its use have flooded market with THC-containing unverifiable provenance frequently contaminated by trace metals residual solvents. Under non-optimized, controlled, harsh reaction conditions, these acid-catalyzed transformations yield cannabinoids including Δ9-THC Δ8-THC, along numerous side products. These are rarely identified quantified accurately, their safety pharmacology remain largely unknown. Aims: This review aims to present an up-to-date understanding one fundamental cannabinoid chemistry: cyclization THC. knowledge will facilitate development safer, cleaner, more affordable, accessible while guiding medical practitioners regulators. Materials Methods: We conducted literature studies published over 5-6 years on interconversion Our focused following key aspects: (1) advances mechanisms optimizing desirable outcomes; (2) new catalysts, "green chemistry" approaches such as solid-supported acids; (3) implementation fit-for-purpose analytical methods better characterize outcomes reassess accuracy cannabis hemp product labeling. Results: Provided strict quality controls materials, isolation techniques, latest research shows that it is feasible access elevated consistently high quality, enriched either THC fractions, cost-effective manner. Among spectrum possible products, easy low-potency compositions may be particularly relevant for serving needs patients consuming hemp-derived dose titration well supporting safe responsible recreational markets now saturated overly potent

Language: Английский

Citations

0
New perspectives on THCA decarboxylation and accurate GC–MS quantitation of Total THC in Cannabis using analyte protectants DOI

Jerome Mulloor,

Walter B. Wilson, Lane C. Sander

et al.

Forensic Chemistry, Journal Year: 2025, Volume and Issue: unknown, P. 100668 - 100668

Published: May 1, 2025

Language: Английский

Citations

0
A Story of Falsification and Authentication: Authenticity Control of Phytopharmaceuticals and Herbal Remedies DOI Creative Commons
Justine Raeber, Christian Steuer

European Journal of Pharmaceutical Sciences, Journal Year: 2025, Volume and Issue: unknown, P. 107136 - 107136

Published: May 1, 2025

Language: Английский

Citations

0
De Novo Regeneration of Cannabis sativa cv. Cheungsam and Evaluation of Secondary Metabolites of Its Callus DOI Creative Commons

S. M. Ahsan,

Da Bin Kwon,

Md. Injamum-Ul-Hoque

et al.

Horticulturae, Journal Year: 2024, Volume and Issue: 10(12), P. 1331 - 1331

Published: Dec. 12, 2024

Cannabis sativa L. cv. ‘Cheungsam’ is an industrial hemp plant of Republic Korea origin, primarily cultivated for fiber and seed production. In vitro germination tissue culture are valuable tools developing various biotechnological techniques. the present study, we aimed to develop a process plants using Cheungsam as model examine secondary metabolites produced from its callus. We also developed method prepare pathogen-free seedlings field-derived seeds hydrogen peroxide (H2O2) solution liquid medium. Treating with removed coat in 3% H2O2 significantly reduced contamination rate. Callus formation de novo organogenesis shoots roots callus were successfully achieved cotyledon leaf tissues prepared seedlings. The most effective regeneration results obtained Murashige Skoog (MS) medium supplemented certain targeted growth regulators. An optimal combination 0.5 mg/L thidiazuron (TDZ) 1.0 1-naphthalene acetic acid proved highly induction. addition TDZ MS stimulated shoot proliferation, while robust root development was best supported by 2.5 indole-3-butyric both explants. Finally, gas chromatography–mass spectrometry (GC–MS) analysis ethanol extract revealed presence different metabolites, including 9-octadecenamide, methyl salicylate, dodecane, tetradecane, phenol, 2,4-bis-(1,1-dimethylethyl). This study provides comprehensive protocol insight into metabolite profiles

Language: Английский

Citations

1
Cost-efficient analysis of cannabinoids in therapeutic oils using HPLC with UV and mass spectrometry detection DOI
Alexander Fries, Carlos Alberto Moldes, Laura S. Mazzaferro

et al.

Natural Product Research, Journal Year: 2024, Volume and Issue: unknown, P. 1 - 9

Published: Dec. 13, 2024

Cannabis oil, derived from

Language: Английский

Citations

0