Integrated Transcriptomic and Metabolomic Analysis of G. hirsutum and G. barbadense Responses to Verticillium Wilt Infection DOI Open Access

Baoguang Xing,

Pengtao Li,

Yanfang Li

et al.

International Journal of Molecular Sciences, Journal Year: 2024, Volume and Issue: 26(1), P. 28 - 28

Published: Dec. 24, 2024

Verticillium wilt (VW) caused by dahliae (Vd) is a devastating fungal cotton disease characterized high pathogenicity, widespread distribution, and frequent variation. It leads to significant losses in both the yield quality of cotton. Identifying key non-synonymous single nucleotide polymorphism (SNP) markers crucial genes associated with VW resistance Gossypium hirsutum barbadense, subsequently breeding new disease-resistant varieties, are essential for management. Here, we sequenced transcriptome metabolome roots TM-1 (G. hirsutum) Hai7124 barbadense) after 0, 1, 2 days V991 inoculation. Transcriptome analysis identified total 72,752 genes, 5814 differentially expressed (DEGs) determined through multiple group comparisons. KEGG enrichment revealed that pathways enriched DEGs obtained from longitudinal transverse comparisons contained glutathione metabolism pathway. Metabolome 995 metabolites, 22 accumulated metabolites (DAMs), which were correlated including metabolism, degradation valine, leucine, isoleucine, biosynthesis terpenoids, alkaloids, pyridine, piperidine. The conjoint transcriptomic metabolomic sequencing DAMs pathway, candidate gene GH_D11G2329 (glutathione S-transferase, GSTF8) potentially response infection was selected. These findings establish basis investigating mechanisms underlying plant’s VW.

Language: Английский

Development of a Rapid and Efficient Protocol for Seed Germination and Seedling Establishment of <em>Oryza coarctata</em> DOI Creative Commons
Lamis Berqdar, Mohamed A. Salem, Jian You Wang

et al.

BIO-PROTOCOL, Journal Year: 2025, Volume and Issue: 15(1365)

Published: Jan. 1, 2025

Seed germination is a critical and challenging process in the propagation of Oryza coarctata, wild halophytic rice species. This protocol outlines seed procedure for O. coarctata. All steps required optimal seedling establishment coarctata both sterile soil-based systems are described detail. Additionally, includes an analysis primary hormones, abscisic acid (ABA) gibberellin (GA), involved regulating dormancy germination. Key features • provides detailed instructions germinating improving its rate. The enables successful vegetative reproductive growth soil. hormones (abscisic gibberellin) that play key roles process. Graphical overview Protocol germination, development, hormone seeds. Step-by-step include seeds on filter papers or growing medium; established seedlings can be grown controlled environments greenhouse. Extraction endogenous offer insights into their controlling

Language: Английский

Citations

0
Integrated Transcriptomic and Metabolomic Analysis of G. hirsutum and G. barbadense Responses to Verticillium Wilt Infection DOI Open Access

Baoguang Xing,

Pengtao Li,

Yanfang Li

et al.

International Journal of Molecular Sciences, Journal Year: 2024, Volume and Issue: 26(1), P. 28 - 28

Published: Dec. 24, 2024

Verticillium wilt (VW) caused by dahliae (Vd) is a devastating fungal cotton disease characterized high pathogenicity, widespread distribution, and frequent variation. It leads to significant losses in both the yield quality of cotton. Identifying key non-synonymous single nucleotide polymorphism (SNP) markers crucial genes associated with VW resistance Gossypium hirsutum barbadense, subsequently breeding new disease-resistant varieties, are essential for management. Here, we sequenced transcriptome metabolome roots TM-1 (G. hirsutum) Hai7124 barbadense) after 0, 1, 2 days V991 inoculation. Transcriptome analysis identified total 72,752 genes, 5814 differentially expressed (DEGs) determined through multiple group comparisons. KEGG enrichment revealed that pathways enriched DEGs obtained from longitudinal transverse comparisons contained glutathione metabolism pathway. Metabolome 995 metabolites, 22 accumulated metabolites (DAMs), which were correlated including metabolism, degradation valine, leucine, isoleucine, biosynthesis terpenoids, alkaloids, pyridine, piperidine. The conjoint transcriptomic metabolomic sequencing DAMs pathway, candidate gene GH_D11G2329 (glutathione S-transferase, GSTF8) potentially response infection was selected. These findings establish basis investigating mechanisms underlying plant’s VW.

Language: Английский

Citations

0