Journal of Agricultural and Food Chemistry,
Journal Year:
2024,
Volume and Issue:
72(27), P. 15357 - 15365
Published: June 28, 2024
The
prohibition
of
processed
animal
proteins
(PAPs)
has
been
relaxed
gradually
since
2007.
official
control
method
for
PAPs
in
feedingstuff,
a
combination
light
microscopy
(LM)
followed
by
PCR,
is
no
longer
sufficient.
Thus,
targeted
LC–MS/MS
was
developed,
which
enables
tissue-specific
distinction
between
egg
and
dairy
products,
gelatine,
derived
from
blood
or
muscle
tissue
the
species
ruminants,
pigs,
poultry,
fish.
Tissue-specific
were
analyzed
after
tryptic
digestion
to
peptides
with
high-resolution
ESI-QTOF-MS.
A
developed
based
on
untargeted
proteomics
approaches
selection
specific
(45
unique
total).
Proficiency
testing
blank
spiked
samples
revealed
excellent
results
trueness
selectivity.
Furthermore,
sensitivity
achieved
at
level
0.1%
(w/w)
assessed
peptides.
Summing
up,
seems
be
suitable
routine
analysis
verification
ring
trials.
International Journal of Cancer,
Journal Year:
2022,
Volume and Issue:
153(2), P. 252 - 264
Published: Nov. 21, 2022
Monoclonal
antibodies
(mAbs)
acting
as
immune
checkpoint
inhibitors
(ICIs)
are
among
the
most
frequently
used
immunotherapies
in
oncology.
However,
precision
medicine
approaches
to
adapt
treatment
patient
still
poorly
exploited.
Given
risk
of
severe
adverse
reactions,
predicting
eligibility
for
ICI
therapy
represents
a
great
asset
medicine.
Today,
extended
panel
mass
spectrometric
approaches,
accompanied
by
newly
developed
sample
preparation
methods
is
strategy
choice
responder
and
non-responder
stratification
on
molecular
basis,
early
detection
resistance.
In
this
perspective
article,
we
review
biodisposition
mAbs,
interest
patients
treated
with
these
possible
analytical
strategies
achieve
goal,
major
emphasis
approaches.
RSC Advances,
Journal Year:
2023,
Volume and Issue:
13(2), P. 1115 - 1124
Published: Jan. 1, 2023
Sialyllactose
(SL)
is
the
most
abundant
acidic
oligosaccharide
in
human
breast
milk
and
plays
a
primary
role
various
biological
processes.
Recently,
SL
has
attracted
attention
as
an
excellent
dietary
supplement
for
arthritis
because
it
effective
cartilage
protection
treatment.
Despite
superior
function
of
SL,
there
are
few
pharmacological
studies
according
to
blood
concentrations
models.
In
this
study,
we
investigated
quantitative
changes
sialic
acids
plasma
obtained
from
mini-pigs
with
osteoarthritis
throughout
exogenous
administration
using
liquid
chromatography-multiple
reaction
monitoring
mass
spectrometry.
Plasma
SL-fed
group
showed
significant
difference
compared
control
group.
Mini
pigs
were
fed
only
Neu5Ac
bound
but
concentration
patterns
two
types
acid,
Neu5Gc,
similar.
addition,
relative
mRNA
expression
level
matrix
metalloproteinases
(MMPs),
which
known
critical
factor
degradation,
was
remarkably
decreased
synovial
membrane
Consequently,
temporal
profiling
suggests
that
can
be
metabolized
utilized
body
may
protect
against
degradation
by
suppressing
MMP
during
progression.
Molecules,
Journal Year:
2021,
Volume and Issue:
26(23), P. 7170 - 7170
Published: Nov. 26, 2021
Quantitative
and
qualitative
analyses
of
cell
protein
composition
using
liquid
chromatography/tandem
mass
spectrometry
are
now
standard
techniques
in
biological
clinical
research.
However,
the
quantitative
analysis
protein-protein
interactions
(PPIs)
cells
is
also
important
since
these
bases
many
processes,
such
as
cycle
signaling
pathways.
This
paper
describes
application
Skyline
software
for
identification
quantification
biotinylated
form
biotin
acceptor
peptide
(BAP)
tag,
which
a
marker
vivo
PPIs.
The
tag
was
used
Proximity
Utilizing
Biotinylation
(PUB)
method,
based
on
co-expression
BAP-X
BirA-Y
mammalian
cells,
where
X
or
Y
interacting
proteins
interest.
A
high
level
biotinylation
detected
model
experiments
were
pluripotency
transcription
factors
Sox2
Oct4,
heterochromatin
HP1γ.
MRM
data
processed
by
normalized
recalculated.
Ratios
levels
experiment
versus
controls
86
±
6
(3
h
time)
71
5
(9
BAP-Sox2
+
BirA-Oct4
32
3
(4
BAP-HP1γ
BirA-HP1γ
experiments.
can
be
applied
PPIs
from
shotgun
proteomics
downloaded
publicly
available
datasets
repositories.
Journal of Agricultural and Food Chemistry,
Journal Year:
2024,
Volume and Issue:
72(27), P. 15357 - 15365
Published: June 28, 2024
The
prohibition
of
processed
animal
proteins
(PAPs)
has
been
relaxed
gradually
since
2007.
official
control
method
for
PAPs
in
feedingstuff,
a
combination
light
microscopy
(LM)
followed
by
PCR,
is
no
longer
sufficient.
Thus,
targeted
LC–MS/MS
was
developed,
which
enables
tissue-specific
distinction
between
egg
and
dairy
products,
gelatine,
derived
from
blood
or
muscle
tissue
the
species
ruminants,
pigs,
poultry,
fish.
Tissue-specific
were
analyzed
after
tryptic
digestion
to
peptides
with
high-resolution
ESI-QTOF-MS.
A
developed
based
on
untargeted
proteomics
approaches
selection
specific
(45
unique
total).
Proficiency
testing
blank
spiked
samples
revealed
excellent
results
trueness
selectivity.
Furthermore,
sensitivity
achieved
at
level
0.1%
(w/w)
assessed
peptides.
Summing
up,
seems
be
suitable
routine
analysis
verification
ring
trials.
