Frontiers in Medicine,
Journal Year:
2025,
Volume and Issue:
12
Published: April 8, 2025
Modern
healthcare
depends
fundamentally
on
clinical
biochemistry
for
disease
diagnosis
and
therapeutic
guidance.
The
discipline
encounters
operational
constraints,
including
sampling
inefficiencies,
precision
limitations,
expansion
difficulties.
Recent
advancements
in
established
technologies,
such
as
mass
spectrometry
the
development
of
high-throughput
screening
point-of-care
are
revolutionizing
industry.
biosensor
technology
wearable
monitors
facilitate
continuous
health
tracking,
Artificial
Intelligence
(AI)/machine
learning
(ML)
applications
enhance
analytical
capabilities,
generating
predictive
insights
individualized
treatment
protocols.
However,
concerns
regarding
algorithmic
bias,
data
privacy,
lack
transparency
decision-making
("black
box"
models),
over-reliance
automated
systems
pose
significant
challenges
that
must
be
addressed
responsible
AI
integration.
limitations
remain-substantial
implementation
expenses,
system
incompatibility
issues,
information
security
vulnerabilities
intersect
with
ethical
considerations
fairness
protected
information.
Addressing
these
demands
coordinated
efforts
between
clinicians,
scientists,
technical
specialists.
This
review
discusses
current
biochemistry,
explicitly
addressing
reference
intervals
barriers
to
implementing
innovative
biomarkers
medical
settings.
discussion
evaluates
how
advanced
technologies
multidisciplinary
collaboration
can
overcome
constraints
while
identifying
research
priorities
diagnostic
accessibility
better
delivery.
Archiv der Pharmazie,
Journal Year:
2025,
Volume and Issue:
358(3)
Published: March 1, 2025
Quantitative
proteomics,
an
integral
subfield
within
is
pivotal
for
elucidating
complex
biological
processes.
By
integrating
with
other
omics
data,
quantitative
proteomics
facilitates
system-level
analysis
and
significantly
advances
our
understanding
of
cellular
networks
disease
mechanisms.
The
ongoing
advancements
in
technology
boost
its
importance
by
improving
analytical
accuracy.
This
review
focuses
on
employing
liquid
chromatography-mass
spectrometry
(LC-MS),
a
cornerstone
technique
renowned
sensitivity,
selectivity,
accuracy,
throughput.
efficacy
LC-MS
heavily
reliant
sample
preparation,
which
encompasses
protein
extraction,
total
estimation,
reduction,
alkylation,
digestion,
cleanup.
For
the
very
first
time,
this
article
provides
detailed
examination
preparation
methods
offering
insights
guidelines
that
researchers
can
utilize
to
refine
their
experimental
protocols
were
not
critically
evaluated
before.
optimizing
workflows,
enhance
robustness
reproducibility
proteomic
studies.
complexities
optimize
workflow
improve
results.
comprehensive
overview
strategies
using
LC-MS,
discussing
underlying
principles
key
considerations
each
step.
delving
into
aims
aid
workflows
achieve
robust
reproducible
results,
ultimately
drive
innovations
breakthroughs
biomedical
research
healthcare.
iScience,
Journal Year:
2025,
Volume and Issue:
28(4), P. 112230 - 112230
Published: March 16, 2025
Immunotherapy
has
transformed
the
landscape
of
cancer
treatment
but
remains
largely
ineffective
for
patients
with
pancreatic
ductal
adenocarcinoma
(PDAC).
Some
patients,
however,
show
improved
outcomes
when
treated
a
combination
immunotherapy
and
chemotherapy.
Here,
we
conducted
deep
serum
proteome
analysis
to
investigate
protein
profiles
PDAC
changes
during
this
combinatorial
treatment.
Utilizing
an
advanced
workflow,
quantified
1,011
proteins
across
211
samples
from
62
patients.
Glycolytic
enzymes
were
associated
survival
in
anti-PD-1-treated
their
abundances
significantly
correlating
expression
levels
tumor
biopsies.
Notably,
set
biomarkers
was
found
be
highly
predictive
(area
under
curve
[AUC]
=
0.91).
Overall,
our
data
demonstrate
potential
proteomics
precision
medicine,
offering
powerful
tool
guide
patient
selection
through
minimally
invasive
biomarker
measurements.
ABSTRACT
Bronchoalveolar
lavage
fluid
(BALF)
has
long
been
used
for
diagnosing
various
lung
diseases
through
its
cellular
components.
However,
the
clinical
utility
of
biomolecules
in
BALF
remains
largely
unexplored.
Recently,
mass
spectrometry‐based
proteomics
applied
to
profile
proteomes
identify
novel
biomarkers
diseases.
This
review
discusses
current
progress
field
and
highlights
potential
as
a
valuable
source
different
Additionally,
we
explored
latest
advancements
findings
from
studies.
Finally,
address
limitations
propose
future
directions
research
opportunities
advance
study
BALF.
Chemical Science,
Journal Year:
2025,
Volume and Issue:
unknown
Published: Jan. 1, 2025
We
report
here
a
two-component
proteolysis
targeting
chimeras
(PROTACs)
strategy
selectively
O-GalNAcylated
and
O-GlcNAcylated
proteins
for
proteasomal
degradation,
which
leads
to
severe
toxicity
in
human
cancer
cell
lines
through...
Frontiers in Medicine,
Journal Year:
2025,
Volume and Issue:
12
Published: April 8, 2025
Modern
healthcare
depends
fundamentally
on
clinical
biochemistry
for
disease
diagnosis
and
therapeutic
guidance.
The
discipline
encounters
operational
constraints,
including
sampling
inefficiencies,
precision
limitations,
expansion
difficulties.
Recent
advancements
in
established
technologies,
such
as
mass
spectrometry
the
development
of
high-throughput
screening
point-of-care
are
revolutionizing
industry.
biosensor
technology
wearable
monitors
facilitate
continuous
health
tracking,
Artificial
Intelligence
(AI)/machine
learning
(ML)
applications
enhance
analytical
capabilities,
generating
predictive
insights
individualized
treatment
protocols.
However,
concerns
regarding
algorithmic
bias,
data
privacy,
lack
transparency
decision-making
("black
box"
models),
over-reliance
automated
systems
pose
significant
challenges
that
must
be
addressed
responsible
AI
integration.
limitations
remain-substantial
implementation
expenses,
system
incompatibility
issues,
information
security
vulnerabilities
intersect
with
ethical
considerations
fairness
protected
information.
Addressing
these
demands
coordinated
efforts
between
clinicians,
scientists,
technical
specialists.
This
review
discusses
current
biochemistry,
explicitly
addressing
reference
intervals
barriers
to
implementing
innovative
biomarkers
medical
settings.
discussion
evaluates
how
advanced
technologies
multidisciplinary
collaboration
can
overcome
constraints
while
identifying
research
priorities
diagnostic
accessibility
better
delivery.
