Quantifying extracellular vesicle heterogeneity: the effect of process conditions on protein cargo for skin therapy

Stem Cell Research & Therapy, Journal Year: 2025, Volume and Issue: 16(1)

Published: May 4, 2025

Extracellular vesicles (EVs) contain a variety of proteins with anti-inflammatory and immunomodulatory properties that offer promising benefits in skin therapy applications. An influx EV proteomic studies recent years has created the opportunity for detailed comparison heterogeneity between context therapeutic Although several process conditions are known to cause variability EVs, little been done quantify impact these factors on nature protein cargo. This review aims both compile publicly available proteomics data quantitatively estimate. cargo-particularly Of roughly 400 articles, 52 relevant were identified within last 15 years. Across studies, 40% 13,000 observed only single study. EVs general found be highly variable, mixed effects models able account 25-60% variance when considering such as source, medium, isolation method, LC-MS ionization, search algorithm. Overall, MSC-derived contained greater fraction pathways associated wound healing (immune system, hemostasis, extracellular matrix organization, cellular response stress) well most number unique compared all other analysed EVs. tool therapeutics, overall cargo underscores need standardized methodologies fully elucidate

Language: Английский

Extracellular vesicles—An omics view

PROTEOMICS, Journal Year: 2024, Volume and Issue: 24(11)

Published: April 26, 2024