The dawn of the revolution that will allow us to precisely describe how microbiomes function DOI Creative Commons
Jean Armengaud

Journal of Proteomics, Journal Year: 2025, Volume and Issue: 316, P. 105430 - 105430

Published: March 11, 2025

The community of microorganisms inhabiting a specific environment, such as the human gut - including bacteria, fungi, archaea, viruses, protozoa, and others is known microbiota. A holobiont, in turn, refers to an integrated ecological unit where microbial communities function interact with their host, thus more integrative concept. To understand processes involved, diversity present must be identified molecular components quantified, especially proteins. Indeed, proteins through roles catalytic units, structural components, signaling molecules are main drivers biological processes. Metagenomics has significantly expanded what we know about genetic material microbiota, revealing functional potential; metabolomics delivers overall snapshot metabolites produced by community. But metaproteomics offers complementary approach explore microbiome holobiont functionality focusing on active pathways from each taxon. Significant recent advances high-resolution tandem mass spectrometry have greatly catalog peptide sequences accessible sample, creating conditions for unprecedented taxonomical profiling, while also providing accurate biomass quantification, detailed protein characterization, greater capacity monitor abundance distinguish host biomarkers. By integrating artificial intelligence into pipeline, extended datasets can now efficiently mined gain comprehensive view complex systems, paving way next-generation metaproteomics. In this perspective, I discuss transformative potential methodology. We cusp remarkable omic revolution that promises uncover intricate workings microbiomes producing vast array new knowledge multiple applications. SIGNIFICANCE: Metaproteomics provides powerful lens investigate identifying quantifying within Recent breakthroughs dramatically repertoire detectable per sample. This progress enables taxonomic resolution identification, precise monitoring, unique identification commentary, delve distinctive features make tool. advancements argue primary challenge analyzing samples shifting data acquisition interpretation. With integration intelligence, believe poised become next Big Thing research, unlocking profound insights ecosystem dynamics.

Language: Английский

The dawn of the revolution that will allow us to precisely describe how microbiomes function DOI Creative Commons
Jean Armengaud

Journal of Proteomics, Journal Year: 2025, Volume and Issue: 316, P. 105430 - 105430

Published: March 11, 2025

The community of microorganisms inhabiting a specific environment, such as the human gut - including bacteria, fungi, archaea, viruses, protozoa, and others is known microbiota. A holobiont, in turn, refers to an integrated ecological unit where microbial communities function interact with their host, thus more integrative concept. To understand processes involved, diversity present must be identified molecular components quantified, especially proteins. Indeed, proteins through roles catalytic units, structural components, signaling molecules are main drivers biological processes. Metagenomics has significantly expanded what we know about genetic material microbiota, revealing functional potential; metabolomics delivers overall snapshot metabolites produced by community. But metaproteomics offers complementary approach explore microbiome holobiont functionality focusing on active pathways from each taxon. Significant recent advances high-resolution tandem mass spectrometry have greatly catalog peptide sequences accessible sample, creating conditions for unprecedented taxonomical profiling, while also providing accurate biomass quantification, detailed protein characterization, greater capacity monitor abundance distinguish host biomarkers. By integrating artificial intelligence into pipeline, extended datasets can now efficiently mined gain comprehensive view complex systems, paving way next-generation metaproteomics. In this perspective, I discuss transformative potential methodology. We cusp remarkable omic revolution that promises uncover intricate workings microbiomes producing vast array new knowledge multiple applications. SIGNIFICANCE: Metaproteomics provides powerful lens investigate identifying quantifying within Recent breakthroughs dramatically repertoire detectable per sample. This progress enables taxonomic resolution identification, precise monitoring, unique identification commentary, delve distinctive features make tool. advancements argue primary challenge analyzing samples shifting data acquisition interpretation. With integration intelligence, believe poised become next Big Thing research, unlocking profound insights ecosystem dynamics.

Language: Английский

Citations

0