Early prediction of spontaneous preterm birth before 34 gestational weeks based on a combination of inflammation-associated plasma proteins DOI Creative Commons
Maria Svenvik, Johanna Raffetseder, Lars Brudin

et al.

Frontiers in Immunology, Journal Year: 2024, Volume and Issue: 15

Published: July 15, 2024

In order to identify and possibly offer prophylactic treatment women at risk for preterm birth (PTB), novel prediction models PTB are needed. Our objective was utilize high-sensitive plasma protein profiling investigate whether early of spontaneous (sPTB) before 34 gestational weeks (gw) possible in a low-risk population. A case-control study conducted on 46 with sPTB gw normal pregnancies term deliveries. Prospectively collected sampled 11 (range 7-16) 25 23-30) analyzed high-sensitivity Proximity Extension Assay levels 177 inflammation-associated proteins, statistically processed multivariate logistic regression analysis. the first trimester, higher hepatocyte growth factor (HGF) were associated <34 (OR 1.49 (1.03-2.15)). second interleukin (IL)-10 2.15 (1.18-3.92)), IL-6 2.59 (1.34-4.99)), receptor activator nuclear κB (RANK) 2.18 (1.26-3.77)) gw. The area under curve including these proteins 0.653 (0.534-0.759) trimester 0.854 (0.754-0.925) trimester. combination from pregnancy showed good predictive ability regarding gw, suggesting it could be valuable supplement assessment clinical sPTB. However, although high number (n=177) method, remains elusive.

Language: Английский

Blood plasma proteomic markers of Alzheimer’s disease, current status and application prospects DOI
Polina A. Strelnikova, Natalia V. Zakharova, А. С. Кононихин

et al.

Expert Review of Proteomics, Journal Year: 2025, Volume and Issue: 22(1), P. 11 - 18

Published: Jan. 2, 2025

Introduction Identifying early risks of developing Alzheimer's disease (AD) is a major challenge as the number patients with AD steadily increases and requires innovative solutions. Current molecular diagnostic modalities, such cerebrospinal fluid (CSF) testing positron emission tomography (PET) imaging, exhibit limitations in their applicability for large-scale screening. In recent years, there has been marked shift toward development blood plasma-based tests, which offer more accessible clinically viable alternative widespread use. Furthermore, advances proteomics technologies have boosted an interest identifying novel biomarkers panels AD-associated proteins.

Language: Английский

Citations

0
Serum Immune-Response Protein Biomarkers Based on Olink Technology for Diagnosis of Ischemic Stroke DOI
Han Wang, Tian Zhao, Jingjing Zeng

et al.

Journal of Proteome Research, Journal Year: 2025, Volume and Issue: unknown

Published: Feb. 3, 2025

The immune response plays a crucial role in the treatment of ischemic stroke (IS). Our primary objective was to explore proteins related and develop noninvasive diagnostic panel. We used high-throughput Olink immunoassay platform quantitatively measure 92 serum 88 patients with IS controls. first selected feature using least absolute shrinkage selection operator (LASSO), random forest (RF), support vector machine (SVM), then modeled them for external validation IS. In this study, we found that 53 exhibited significant differences compared control group. GLB1, PRDX5, DDX58, CLEC4C as potential protein biomarkers differentiate from group LASSO, RF, SVM. model, which included these four proteins, demonstrated excellent performance data sets, achieving an AUC value 0.899 (95%CI: 0.848-0.950). findings offer valuable insights into both diagnosis These results novel approach clinical decision-making

Language: Английский

Citations

0
Distinct Alterations of Inflammatory Biomarkers in Cluster Headache: A Case Control Study DOI Creative Commons
Nunu Lund, Connar Stanley James Westgate, M. Søborg

et al.

Annals of Neurology, Journal Year: 2025, Volume and Issue: unknown

Published: Feb. 21, 2025

Objective Investigate the immune system's role in cluster headache by analyzing cytokines people with and headache‐free controls, explore if certain could predict a specific phenotype. Methods We measured 45 adult participants from Danish Cluster Headache Biobank prospective case–control setup. People were diagnosed according to International Classification of Disorders third‐edition. Controls matched for age sex. Results A total 412 analyzed deriving 99 chronic headache, 108 episodic (ECH) bout, 105 ECH remission, 100 successfully controls. Compared 13 altered bout ( p < 0.05), 3 remission 10 0.05). Oncostatin m was significantly elevated all disease states compared controls Overall, investigated showed distinct patterns alterations between and, interestingly, IL‐1β associated having rather than logistic regression model adjusting potential confounders Interpretation Findings show that system is elevated, constituting promising target future studies. The are striking urges further research better understand its prediction activity treatment response. ANN NEUROL 2025

Language: Английский

Citations

0
Computational tools in genomics and proteomics DOI
Sharav Desai,

Vipul Patel,

Kunal Bhosle

et al.

Elsevier eBooks, Journal Year: 2025, Volume and Issue: unknown, P. 489 - 518

Published: Jan. 1, 2025

Language: Английский

Citations

0
Pemphigoid disease model systems for clinical translation DOI Creative Commons

Michael Tigges,

Sören Dräger,

Ilaria Piccini

et al.

Frontiers in Immunology, Journal Year: 2025, Volume and Issue: 16

Published: March 17, 2025

Pemphigoid diseases constitute a group of organ-specific autoimmune characterized and caused by autoantibodies targeting autoantigens expressed in the skin mucous membranes. Current therapeutic options are still based on unspecific immunosuppression that is associated with severe adverse events. Biologics, IL4-pathway or IgE expected to change treatment landscape pemphigoid diseases. However, clinical studies demonstrated these pathways alone most likely not sufficient meet patient healthcare partitioners expectations. Hence, model systems needed identify validate novel targets These include pre-clinical animal models, vitro ex vivo systems, hypothesis-driven drug repurposing, as well exploitation real-world-data. In this review, we will highlight medical need for diseases, in-depth discuss advantages disadvantages available disease systems. Ultimately, how rapid translation can be achieved benefit patients.

Language: Английский

Citations

0
The Association of Serum Biomarkers With Symptomatic Hemorrhagic Transformation in Acute Ischemic Stroke Patients: A Combined Retrospective and Prospective Study DOI Creative Commons
Shuhua Yuan, Daiquan Gao, Wenjuan Shi

et al.

CNS Neuroscience & Therapeutics, Journal Year: 2025, Volume and Issue: 31(3)

Published: March 1, 2025

Symptomatic intracranial hemorrhage transformation (s-HT) is a serious complication of ischemic stroke, leading to early neurological deterioration and poor prognosis. It an urgent problem timely effectively identify high-risk patients with s-HT at the stage stroke. However, so far, there are no effective clinical detection methods or measures. Therefore, present study aimed explore novel blood biomarkers related s-HT. This includes two parts: retrospective prospective cohort study. In first part, were screened (n = 18), non-s-HTs 128) selected from same period case in cohort. The baseline samples obtained within 30 min admission, levels 92 proteins cerebrovascular diseases detected using Olink proteomics technology. Multivariate logistic regression receiver operating characteristic curves used analyze relationship between serum biomarker second 28) 130) cohort, which met criteria for inclusion exclusion. Enzyme-linked immunosorbent assay (ELISA) was measure potential biomarker(s) part confirm its/their association(s) showed that had lower von Willebrand factor (vWF) higher osteoprotegerin, phospholipase C, human insulin-like growth binding protein-7, matrix metalloproteinase-2, galectin-4, spondin-1 than (p < 0.005) Principal component (PC) analysis seven could explain 62.76% variance set, vWF main loading PC2. low level independent risk 0.05) after adjusting confounders. ELISA test results significantly non-s-HT group (26.57 [13.64-37.18] vs. 42.00 [26.02-55.52] ng/mL, p 0.001) Incorporating into factors improved accuracy predicting (area under curve [AUC, 0.731 0.641, 0.001], 0.747 0.560, 0.001]) compared model employing only both cohorts. Through use combined study, might be occurrence stroke patients.

Language: Английский

Citations

0
Seasonal Proteomic Variations and Biomarkers in Seasonal Allergic Rhinitis: Insights from Olink Inflammation Profiling DOI Creative Commons

Jiaqi Shen,

Xinliang Zheng,

Mengting Yan

et al.

Journal of Inflammation Research, Journal Year: 2025, Volume and Issue: Volume 18, P. 6191 - 6202

Published: May 1, 2025

Seasonal allergic rhinitis (SAR) is a prevalent inflammatory condition, yet its molecular mechanisms and reliable biomarkers remain incompletely understood. This study aimed to identify key inflammation-related proteins pathways associated with SAR by investigating seasonal proteomic profile variations their correlations symptoms. Serum samples were collected from nineteen patients during both allergy (in-season, IS) non-allergy (out-of-season, OS) periods. Differentially expressed (DEPs) identified using the Olink Target 96 Inflammation panel, which further analyzed through Gene Ontology (GO) Kyoto Encyclopedia of Genes Genomes (KEGG) pathway enrichment analyses. Spearman correlation analysis was conducted explore associations between DEPs symptoms, including sneezing, rhinorrhea, nasal blockage, itchy nose, eye. A total 36 identified, all significantly upregulated in season. Notable such as glial cell line-derived neurotrophic factor (GDNF), interleukin-18 receptor 1 (IL-18R1), interleukin-15 alpha (IL-15RA) showed strong Sneezing IL-2 beta (IL-2RB) (r = 0.415, p 0.013), rhinorrhea FMS-related tyrosine kinase 3 ligand (Flt3L) 0.455, 0.004), blockage osteoprotegerin (OPG) 0.493, 0.002). GO revealed enrichments Ras signaling small GTPase pathways, while KEGG highlighted immune-related PI3K-Akt cytokine-cytokine interactions. that vary seasonally SAR, offering insights into potential therapeutic targets for management. Further studies are recommended validate these findings larger more diverse populations.

Language: Английский

Citations

0
The Role of Omics Techniques in Diabetic Wound Healing: Recent Insights into the Application of Single-Cell RNA Sequencing, Bulk RNA Sequencing, Spatial Transcriptomics, and Proteomics DOI Creative Commons

Eleftheria-Angeliki Valsami,

Gilbert Chu, Ming Guan

et al.

Advances in Therapy, Journal Year: 2025, Volume and Issue: unknown

Published: May 17, 2025

Language: Английский

Citations

0
Proteomic Markers of Aging and Longevity: A Systematic Review DOI Open Access
Anna A. Kliuchnikova, Ekaterina V. Ilgisonis, Alexander I. Archakov

et al.

International Journal of Molecular Sciences, Journal Year: 2024, Volume and Issue: 25(23), P. 12634 - 12634

Published: Nov. 25, 2024

This article provides a systematic review of research conducted on the proteomic composition blood as part complex biological age estimation. We performed comprehensive analysis 17 publicly available datasets and compiled an integral list proteins. These proteins were sorted based their detection probability using mass spectrometry in human plasma. propose this basis for creating panel peptides quantifying content selected format aging clock. The are especially notable roles inflammatory processes lipid metabolism. Our findings suggest, first time, that associated with systemic disorders, including those approved by FDA clinical use, could serve potential markers aging.

Language: Английский

Citations

2
Early prediction of spontaneous preterm birth before 34 gestational weeks based on a combination of inflammation-associated plasma proteins DOI Creative Commons
Maria Svenvik, Johanna Raffetseder, Lars Brudin

et al.

Frontiers in Immunology, Journal Year: 2024, Volume and Issue: 15

Published: July 15, 2024

In order to identify and possibly offer prophylactic treatment women at risk for preterm birth (PTB), novel prediction models PTB are needed. Our objective was utilize high-sensitive plasma protein profiling investigate whether early of spontaneous (sPTB) before 34 gestational weeks (gw) possible in a low-risk population. A case-control study conducted on 46 with sPTB gw normal pregnancies term deliveries. Prospectively collected sampled 11 (range 7-16) 25 23-30) analyzed high-sensitivity Proximity Extension Assay levels 177 inflammation-associated proteins, statistically processed multivariate logistic regression analysis. the first trimester, higher hepatocyte growth factor (HGF) were associated <34 (OR 1.49 (1.03-2.15)). second interleukin (IL)-10 2.15 (1.18-3.92)), IL-6 2.59 (1.34-4.99)), receptor activator nuclear κB (RANK) 2.18 (1.26-3.77)) gw. The area under curve including these proteins 0.653 (0.534-0.759) trimester 0.854 (0.754-0.925) trimester. combination from pregnancy showed good predictive ability regarding gw, suggesting it could be valuable supplement assessment clinical sPTB. However, although high number (n=177) method, remains elusive.

Language: Английский

Citations

0