Sertraline Alleviates Chronic Prostatitis by Regulating the TRPV1 Channel DOI Creative Commons
Yongfeng Lao,

Yanan Bai,

Xin Guan

et al.

Journal of Inflammation Research, Journal Year: 2024, Volume and Issue: Volume 17, P. 4257 - 4275

Published: July 1, 2024

Introduction: Although sertraline has been widely used for chronic prostatitis (CP), the mechanisms are unclear. Herein, we explored of in treating CP. Methods: Network pharmacology methods were to explore potential targets and molecular mechanisms. LPS was stimulate RWPE-1 cells construct an vitro model An experimental autoimmune (EAP) mice built. CCK-8 assay, EdU BrdU detection, Tunel assay performed evaluate proliferation apoptosis process or tissues, respectively. DCFH-DA Fluo-4 fluorescence probes detect intracellular ROS calcium concentrations. Von Frey filaments open-field tests utilized pain response depressive-like behavior mice. Histopathology evaluated through hematoxylin eosin staining. RT-qPCR, Western blot, immunofluorescence, immunohistochemistry transcription, expression, location related proteins. Molecular dynamics (MD) simulation surface plasmon resonance (SPR) measure binding capacity Results: Through a network analysis, 27 CP obtained, 5 key (CHRM1, ADRA1B, HTR2B, HTR2A, TRPV1) finally identified. Functional experiments suggested that TRPV1 involved proliferation, inhibition, production LPS-induced cells. In showed significantly inhibited cell generation, transcription inflammation cytokines Additionally, markedly promoted level LPS-stimulated elevated expression BAX while reducing levels Bcl2 Caspase-3. MD SPR confirmed direct TRPV1. Moreover, down-regulated influx agonist (Capsaicin) restored effects on apoptosis, production, Mice demonstrated treatment could reduce response, improve depression-like symptoms, relieve local prostate EAP mice, as well TRPV1, inhibit promote tissues Discussion: The results revealed anti-inflammatory effect mechanism regulating channel. It indicated might serve complementary agent Keywords: prostatitis, sertraline, RWPE-1,

Language: Английский

Short-chain fatty acids in Huntington's disease: Mechanisms of action and their therapeutic implications DOI
Mohamed J. Saadh,

Hanan Hassan Ahmed,

Radhwan Abdul Kareem

et al.

Pharmacology Biochemistry and Behavior, Journal Year: 2025, Volume and Issue: unknown, P. 173972 - 173972

Published: Feb. 1, 2025

Language: Английский

Citations

0
The Role of Butirprost® as an Adjuvant in Enhancing the Effect of Antibiotics in Patients Affected by Chronic Bacterial Prostatitis: A Randomized Prospective Trial DOI Creative Commons
Felice Crocetto, Armando Calogero, Michele Santangelo

et al.

Medicina, Journal Year: 2025, Volume and Issue: 61(1), P. 148 - 148

Published: Jan. 17, 2025

Bacterial prostatitis (BP) is a common prostatic infection characterized by pain and urinary symptoms, often with negative bacterial cultures from secretions. It affects young older men bimodally impacts quality of life (QoL) significantly. Background Objectives: Treatment typically involves antibiotics, but multimodal approach additional nutraceuticals may enhance outcomes. This study aimed to assess the efficacy Butirprost® in association fluoroquinolones patients chronic (CBP). Materials Methods: Patients diagnosed (positive Meares-Stamey test symptom duration > 3 months) at University Naples "Federico II", Italy, March 2024 July were included this study. All underwent cultures. randomized into two groups: Group A received antibiotics plus (sodium hyaluronate Plantago major) for one month, while B alone. International Prostatic Symptoms Score (IPSS) National Institutes Health Chronic Prostatitis Symptom Index (NIH-CPSI) questionnaires administered baseline 15 30 days. Results: Out 60 (Group A: 30, B: 30), showed significant improvement IPSS NIH-CPSI scores days compared B. Notable improvements observed pain, QoL. Conclusions: The administration along resulted both scores, affected

Language: Английский

Citations

0
Sodium butyrate attenuates liver fibrogenesis via promoting H4K8 crotonylation DOI
Ruiqi Tang, Hua Zha, Rongrong Liu

et al.

Molecular and Cellular Biochemistry, Journal Year: 2025, Volume and Issue: unknown

Published: April 3, 2025

Language: Английский

Citations

0
Sertraline Alleviates Chronic Prostatitis by Regulating the TRPV1 Channel DOI Creative Commons
Yongfeng Lao,

Yanan Bai,

Xin Guan

et al.

Journal of Inflammation Research, Journal Year: 2024, Volume and Issue: Volume 17, P. 4257 - 4275

Published: July 1, 2024

Introduction: Although sertraline has been widely used for chronic prostatitis (CP), the mechanisms are unclear. Herein, we explored of in treating CP. Methods: Network pharmacology methods were to explore potential targets and molecular mechanisms. LPS was stimulate RWPE-1 cells construct an vitro model An experimental autoimmune (EAP) mice built. CCK-8 assay, EdU BrdU detection, Tunel assay performed evaluate proliferation apoptosis process or tissues, respectively. DCFH-DA Fluo-4 fluorescence probes detect intracellular ROS calcium concentrations. Von Frey filaments open-field tests utilized pain response depressive-like behavior mice. Histopathology evaluated through hematoxylin eosin staining. RT-qPCR, Western blot, immunofluorescence, immunohistochemistry transcription, expression, location related proteins. Molecular dynamics (MD) simulation surface plasmon resonance (SPR) measure binding capacity Results: Through a network analysis, 27 CP obtained, 5 key (CHRM1, ADRA1B, HTR2B, HTR2A, TRPV1) finally identified. Functional experiments suggested that TRPV1 involved proliferation, inhibition, production LPS-induced cells. In showed significantly inhibited cell generation, transcription inflammation cytokines Additionally, markedly promoted level LPS-stimulated elevated expression BAX while reducing levels Bcl2 Caspase-3. MD SPR confirmed direct TRPV1. Moreover, down-regulated influx agonist (Capsaicin) restored effects on apoptosis, production, Mice demonstrated treatment could reduce response, improve depression-like symptoms, relieve local prostate EAP mice, as well TRPV1, inhibit promote tissues Discussion: The results revealed anti-inflammatory effect mechanism regulating channel. It indicated might serve complementary agent Keywords: prostatitis, sertraline, RWPE-1,

Language: Английский

Citations

2