Heterologous expression and functional characterization of Drosophila suzukii OR69a transcript variants unveiled response to kairomones and to a candidate pheromone DOI Creative Commons
Alberto Maria Cattaneo, Peter Witzgall, Charles A. Kwadha

et al.

Research Square (Research Square), Journal Year: 2022, Volume and Issue: unknown

Published: April 21, 2022

Abstract Background Drosophila suzukii is among the main pests of berries, and to which control, primarily through application insecticides challenging urges alternative strategies. Methods based on targeting chemical sensing interfering with behavior this pest insect are promising, facilitated by isolation functional characterization chemosensory receptors aimed identify behaviorally active ligands. In our previous investigations, we functionally characterized subunits OR69a locus melanogaster as being capable bind both pheromones kairomones. well conserved within genus , represents a candidate target for broad behavioral interference communication towards design novel control Results By means heterologous expression in empty ab3A neurons D. together Single Sensillum Recording (SSR), screened three OR69a-subunits have identified (DsuzOR69aA, DsuzOR69aB, DsuzOR69aC) panel 48 We deorphanized these several compounds, including two possible kairomones (3-octanol R-carvone) (Z)-4-nonenal, fly-emitted volatile compound, although different pharmacological qualities. coupling Gas Chromatography (GC-SSR), demonstrated ligands enhance ab3A-spiking at nanogram aliquots complementary fashion variants, another kairomone, methyl salicylate, most specific ligand sole subunit DsuzOR69aB. testing headspaces collected from female fly-associated yeast Hanseniaspora uvarum GC-SSR did not observe activation but yeast. situ hybridization analysis conducted antennae suggested unique into their co-expression same neurons. Conclusions The constitute cation channels, binding suggests kairomone specificity, even if effects may coexist compound (Z)-4-nonenal fashion. Among kairomones, salicylate it be DsuzOR69aB subunit, inspiring future investigation validate potentials

Language: Английский

Exploring future applications of the apiculate yeast Hanseniaspora DOI Creative Commons
Niël van Wyk, Jennifer Badura, Christian von Wallbrunn

et al.

Critical Reviews in Biotechnology, Journal Year: 2023, Volume and Issue: 44(1), P. 100 - 119

Published: Feb. 23, 2023

As a metaphor, lemons get bad rap; however the proverb '

Language: Английский

Citations

21
Chemical and Electrophysiological Characterisation of Headspace Volatiles from Yeasts Attractive to Drosophila suzukii DOI Creative Commons
Irene Castellan, Claire Duménil, Guillermo Rehermann

et al.

Journal of Chemical Ecology, Journal Year: 2024, Volume and Issue: 50(11), P. 830 - 846

Published: May 1, 2024

Abstract Chemical control of Drosophila suzukii (Diptera: Drosophilidae) based on the use insecticides is particularly challenging as insect attacks ripening fruits shortly before harvest. An alternative strategy may rely yeasts phagostimulants and baits, applied canopy attract-and-kill formulations. The aim this research was to identify most attractive among six yeast species for D. : Saccharomyces cerevisiae , Hanseniaspora uvarum Clavispora santaluciae Saccharomycopsis vini Issatchenkia terricola Metschnikowia pulcherrima . volatile profile C described first time. Behavioural experiments identified H. S. yeasts. characterization headspace volatiles using direct (DHS) solid-phase microextraction (SPME) revealed several strain-specific compounds. With DHS injection, 19 were characterised, while SPME 71 compounds constituting headspace. Both analyses terpenoids including β-ocimene, citronellol, ( Z )-geraniol (nerol), geranial distinct constituents further investigated closed-loop stripping analysis (CSLA) electroantennography. Out 14 quantified by CSLA, ethyl acetate, isoamyl β-myrcene, benzaldehyde linalool detected antennae might generate strong attractiveness uvarum. Our results highlight a attraction various associated with both flies their habitat demonstrate how different sampling methods can impact compound characterization. It remains be demonstrated whether special adaptations certain what extent metabolites causing are interchangeable.

Language: Английский

Citations

5
Mycophagy: A Global Review of Interactions between Invertebrates and Fungi DOI Creative Commons
Brianna Santamaria, Annemieke Verbeken, Danny Haelewaters

et al.

Journal of Fungi, Journal Year: 2023, Volume and Issue: 9(2), P. 163 - 163

Published: Jan. 26, 2023

Fungi are diverse organisms that occupy important niches in natural settings and agricultural settings, acting as decomposers, mutualists, parasites pathogens. Interactions between fungi other organisms, specifically invertebrates, understudied. Their numbers also severely underestimated. Invertebrates exist many of the same spaces known to engage fungal feeding or mycophagy. This review aims provide a comprehensive, global view mycophagy invertebrates bring attention areas need more research, by prospecting existing literature. Separate searches on Web Science were performed using terms “mycophagy” “fungivore”. Invertebrate species corresponding extracted from articles retrieved, whether research was field- laboratory-based, location observation if field-based. Articles excluded they did not list at least genus identification for both invertebrates. The search yielded 209 papers covering seven phyla 19 invertebrate orders. Ascomycota Basidiomycota most represented whereas Coleoptera Diptera make up observations. Most field-based observations originated North America Europe. Research is lacking some phyla, orders, geographic regions.

Language: Английский

Citations

11
Differential Attraction of Summer and Winter Morphs of Spotted Wing Drosophila, Drosophila suzukii, to Yeasts DOI Creative Commons
R. Jones, Matthew R. Goddard, Paul E. Eady

et al.

Journal of Chemical Ecology, Journal Year: 2025, Volume and Issue: 51(1)

Published: Feb. 1, 2025

Abstract Drosophila suzukii is a pest of soft and stone fruits that attracted to yeast volatile metabolites. has distinct summer winter morphs which are found in different habitats. Complex communities yeasts likely differ between habitats thus we hypothesized their attraction volatiles from species combinations species. We presented D. with isolation combinations, as either post-culture mixes or co-cultured, measured activity the flies laboratory choice tests Locomotor Activity Monitor proxy for attraction. Candida zemplinina was more attractive than when cultured both sterile strawberry juice (SSJ) artificial culture media (YPD). No significant correlations were principle component scores derived single profiles fly activity. There also differential certain mixes, most notably post SSJ Hanseniaspora uvarum C. consistently morphs. Mixtures Metschnikowia pulcherrima + Pichia pijperi H. M. P. YPD preferred by morphs, whilst co-cultures . However, co-culturing did not enhance compared constituent morph. Differential suggests options new bait formulations integrated management strategies may be tailored better target

Language: Английский

Citations

0
Imidacloprid Resistance Challenges in Brazilian Strains of Drosophila suzukii (Diptera: Drosophilidae) DOI Creative Commons
Felipe Andreazza, Flávio Roberto Mello García,

Pedro Bento da Silva

et al.

Insects, Journal Year: 2025, Volume and Issue: 16(5), P. 494 - 494

Published: May 5, 2025

Drosophila suzukii (Matsumura) is a relevant pest affecting berries and stone fruits globally, including in the Neotropical region, where its invasion was reported about decade ago. Despite chemical control being main management method for D. suzukii, data on insecticide susceptibility populations are scarce. Here, we assessed of three field-collected Brazilian to four insecticides (i.e., deltamethrin, permethrin, spinetoram, imidacloprid) contrasted this with standard insecticide-susceptible population. Using discriminating concentration (LC90) from susceptible population, identified resistant populations. Synergist exposure (piperonyl butoxide, triphenyl phosphate, diethyl maleate) indicated role detoxification enzymes resistance. Our results showed that deltamethrin most toxic, followed by imidacloprid. While all field were similarly pyrethroids spinosyns, one population Minas Gerais Paula Candido) had significantly lower imidacloprid susceptibility, only 53.4 ± 5.2% mortality at 10.0 g/L (the equivalent 10-fold estimated LC90). Only piperonyl butoxide increased Candido flies. findings indicate occurrence cytochrome P450 enzyme-based resistance state Gerais, which can challenge Brazil.

Language: Английский

Citations

0
Yeast and fruit fly mutual niche construction and antagonism against mould DOI Creative Commons
Amrita Chakraborty, Boyd A. Mori, Guillermo Rehermann

et al.

Functional Ecology, Journal Year: 2022, Volume and Issue: 36(7), P. 1639 - 1654

Published: April 9, 2022

Abstract A goal in insect–microbe ecology is to understand the mechanisms regulating species associations and mutualistic interactions. The spotted wing drosophila Drosophila suzukii develops ripening fruit, unlike other drosophilids that typically feed on overripe associated with yeast Hanseniaspora uvarum . We hypothesized D. H. engage niche construction leading a relation, facilitating exploitation of fruit berries as larval substrate. show proliferates both ripe unripe raspberries, mediates attraction larvae adult flies, enhances egg‐laying mated females sufficient food substrate support development. Moreover, suppresses antagonistic grey mould, Botrytis cinerea collaboration D larvae, produces less ethanol than baker's yeast. thus creates favourable conditions for development, which susceptible mould. , hand, vectors suitable substrates such where feeding activity growth Larval also helps suppress B. otherwise outcompetes raspberry, absence fly larvae. In conclusion, development berries, promotes dispersal berries. Yeast modify their shared habitat reciprocal mutual interaction. Read free Plain Language Summary this article Journal blog.

Language: Английский

Citations

16
Field and greenhouse application of an attract‐and‐kill formulation based on the yeast Hanseniaspora uvarum and the insecticide spinosad to control Drosophila suzukii in grapes DOI Creative Commons
Urban Spitaler, Carlo S Cossu,

Lorenz Delle Donne

et al.

Pest Management Science, Journal Year: 2021, Volume and Issue: 78(3), P. 1287 - 1295

Published: Dec. 2, 2021

Abstract BACKGROUND The invasive insect Drosophila suzukii (Matsumura) is an important pest of several red grape varieties. yeast Hanseniaspora uvarum (Niehaus), which associated with D. , strongly attracts flies and stimulates them to feed on yeast‐laden food. In the present study, a formulation based H. culture spinosad insecticide was applied foliage vineyards control compared applying whole plant. After successful application in vineyard, we tested additional ‐based formulations greenhouse determine their capacity . RESULTS Application ‐spinosad at 36.4 g per hectare reduced field infestation same rate as 120 prevented residues grapes. Leaves treated transferred laboratory assay caused high mortality number eggs laid fruits. Formulations showed that both cell‐free supernatant centrifuged increased fly unsprayed control. CONCLUSION comparison typical spray applications, use combination attract‐and‐kill against reduces pesticide fruits by targeting treatment canopy decreasing amount without compromising efficacy.

Language: Английский

Citations

17
Control of spotted wing drosophila (Drosophila suzukii) in sweet cherry and raspberry using bait sprays DOI
R. Noble, Bethan Shaw, Adam S. Walker

et al.

Journal of Pest Science, Journal Year: 2022, Volume and Issue: 96(2), P. 623 - 633

Published: Oct. 6, 2022

Language: Английский

Citations

12
An advanced metabolomic approach on grape skins untangles cultivar preferences by Drosophila suzukii for oviposition DOI Creative Commons
Rémy Marcellin-Gros,

Sébastien Hévin,

Clara Chevalley

et al.

Frontiers in Plant Science, Journal Year: 2024, Volume and Issue: 15

Published: Aug. 21, 2024

Insects’ host preferences are regulated by multiple factors whose interactions only partly understood. Here we make use of an in-depth, untargeted metabolomic approach combining molecular networking (MN) and supervised Analysis variance Multiblock Orthogonal Partial Least Squares (AMOPLS) to untangle egg-laying Drosophila suzukii , invasive, highly polyphagous destructive fruit pest originating from Southeast Asia. Based on behavioural experiments in the laboratory as well field observation, selected eight genetically related Vitis vinifera cultivars (e.g., Ancellotta, Galotta, Gamaret, Gamay, Gamay précoce, Garanoir, Mara Reichensteiner) exhibiting significant differences their susceptibility toward D. . The two most least attractive red were chosen for further analyses grape skins. combination MN statistical AMOPLS findings with semi-quantitative detection information enabled us identify flavonoids interesting markers attractiveness four studied towards Overall, dihydroflavonols accumulated unattractive cultivars, while richer flavonols. Crucially, both flavonols abundant metabolites analysis extracted molecules skin. We discuss how these flavonoid classes might influence behaviour females they could serve potential infestations grapes that can be potentially extended other fruits. believe our novel, integrated analytical also applied study biological relationships characterised evolving parameters.

Language: Английский

Citations

2
Comparative transcriptomic assessment of the chemosensory receptor repertoire of Drosophila suzukii adult and larval olfactory organs DOI Creative Commons
William B. Walker, Boyd A. Mori, Alberto Maria Cattaneo

et al.

Comparative Biochemistry and Physiology Part D Genomics and Proteomics, Journal Year: 2022, Volume and Issue: 45, P. 101049 - 101049

Published: Dec. 6, 2022

The spotted wing Drosophila, Drosophila suzukii, has emerged within the past decade as an invasive species on a global scale, and is one of most economically important pests in fruit berry production Europe North America. Insect ecology, to strong degree, depends chemosensory modalities smell taste. Extensive research sensory receptors olfactory gustatory systems melanogaster provide excellent frame reference better understand fundamentals D. suzukii. This knowledge may enhance development semiochemicals for sustainable management which urgently needed. Here, using transcriptomic approach we report receptor expression profiles suzukii female male antennae, first time, larval heads including dorsal organ that houses neurons. In adults, generally observed lack sexually dimorphic levels antennae. While there was conservation antennal odorant ionotropic orthologues showed more distinct species-specific profiles. head tissues, all three gene families, also greater degree patterns. Analysis repertoires pest species, relative those genetic model enables comparative studies chemosensory, physiology, ecology

Language: Английский

Citations

10