Transcriptional profiling reveals a critical role of GmFT2a in soybean staygreen syndrome caused by the pest Riptortus pedestris

New Phytologist, Journal Year: 2022, Volume and Issue: 237(5), P. 1876 - 1890

Published: Nov. 21, 2022

Summary Soybean staygreen syndrome, characterized by delayed leaf and stem senescence, abnormal pods, aborted seeds, has recently become a serious prominent problem in soybean production. Although the pest Riptortus pedestris received increasing attention as possible cause of mechanism remains unknown. Here, we clarify that direct feeding R. , not transmission pathogen this pest, is primary typical syndrome critical damage occurs at early pod stage . Transcriptome profiling indicated many signal transduction pathways, including photoperiod, hormone, defense response, photosynthesis, respond to infestation. Importantly, discovered members FLOWERING LOCUS T ( FT ) gene family were suppressed infestation, overexpression floral inducer GmFT2a attenuates symptoms mediating response photosynthesis. Together, our findings systematically illustrate association between infestation provide basis for establishing targeted prevention control system.

Language: Английский

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Forecasting the Expansion of Bactrocera tsuneonis (Miyake) (Diptera: Tephritidae) in China Using the MaxEnt Model

Insects, Journal Year: 2024, Volume and Issue: 15(6), P. 417 - 417

Published: June 4, 2024

The invasive pest, Bactrocera tsuneonis (Miyake), has become a significant threat to China’s citrus industry. Predicting the area of potentially suitable habitats for B. is essential optimizing pest control strategies that mitigate its impact on Here, existing distribution data tsuneonis, as well current climate and projections four future periods (2021–2040, 2041–2060, 2061–2080, 2081–2100) from Coupled Model Intercomparison Project Phase 6 (CMIP6) were obtained. under different change scenarios in China was predicted using optimized MaxEnt model, ArcGIS, ENMeval package. accuracy assessed ROC curves, primary environmental factors influencing identified based percent contribution. When regularization multiplier (RM) set 1.5 feature combination (FC) LQH, model with lower complexity Under these parameter settings, mean training AUC 0.9916, testing 0.9854, indicating high predictive performance. most influential variables limiting Precipitation Warmest Quarter (Bio18) Temperature Seasonality (standard deviation ×100) (Bio4). climatic conditions, habitat covered an 215.9 × 104 km2, accounting 22.49% country’s land area. Potentially primarily concentrated Central China, South East China. However, projections, exhibited varying degrees expansion. Furthermore, centroid total this gradually shifted westward northward. These findings suggest will spread northern western regions changes. results our study indicate have major effect invasion implications development

Language: Английский

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Ecological forecasts of insect range dynamics: a broad range of taxa includes winners and losers under future climate

Current Opinion in Insect Science, Journal Year: 2024, Volume and Issue: 62, P. 101159 - 101159

Published: Jan. 9, 2024

Language: Английский

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A salivary secretory protein from Riptortus pedestris facilitates pest infestation and soybean staygreen syndrome

Molecular Plant Pathology, Journal Year: 2023, Volume and Issue: 24(6), P. 560 - 569

Published: March 14, 2023

The bean bug (Riptortus pedestris), one of the most important pests soybean, causes staygreen syndrome, delaying plant maturation and affecting pod development, resulting in severe crop yield loss. However, little is known about underlying mechanism this pest. In study, we found that a salivary secretory protein, Rp614, induced cell death nonhost Nicotiana benthamiana leaves. NbSGT1 NbNDR1 are involved Rp614-induced death. Tissue specificity analysis showed Rp614 mainly present glands highly during pest feeding. RNA interference experiments syndrome caused by R. pedestris was significantly attenuated when silenced. Together, our results indicate plays an essential role infestation provide promising target for control.

Language: Английский

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The intestinal microbial community and function of Riptortus pedestris at different developmental stages and its effects on development

Frontiers in Microbiology, Journal Year: 2025, Volume and Issue: 16

Published: Jan. 22, 2025

Introduction Riptortus pedestris is a destructive pest that threatens multiple leguminous crops in China. The intestinal microbiota plays crucial role the growth and reproduction of host insects. However, composition function gut at different developmental stages remain unclear. Methods Here, metagenomic sequencing was performed to clarify microbial diversity 2nd-, 3rd-, 4th-, 5th- instar nymphs (2 N–5 N) female adults (FAs) R. effects vital bacteria on development detected. have stage specificity, indicating their . Results Enterococcus Caballerronia were predominant present during 2 N–FAs. In addition, abundances 3 N 4 guts significantly greater than those others guts. Furthermore, 5 harbored abundant Burkholderia - Paraburkholderia Caballeronia metabolic pathways enriched from FAs. Carbohydrate metabolism, including glycoside hydrolases (GHs) glycosyl transferases (GTs), occurs throughout entire stage. Many antibiotic resistance genes (ARGs) detected Pseudomonadota Bacillota presented broad spectrum resistance. Excitingly, eliminated by treatment unable molt normally, lifespan shortened nymphs, had significant effect nymph development. Conclusion summary, our results, for first time, systematically illustrate abundance across demonstrate genera are This study provides basis stinkbug management strategies focus pivotal microbiota.

Language: Английский

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Shared phylogeographic patterns and environmental responses of co-distributed soybean pests: Insights from comparative phylogeographic studies of Riptortus pedestris and Riptortus linearis in the subtropics of East Asia

Molecular Phylogenetics and Evolution, Journal Year: 2024, Volume and Issue: 195, P. 108055 - 108055

Published: March 12, 2024

Language: Английский

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Elucidating the ecophysiology of soybean pod-sucking stinkbug Riptortus pedestris (Hemiptera: Alydidae) based on de novo genome assembly and transcriptome analysis

BMC Genomics, Journal Year: 2024, Volume and Issue: 25(1)

Published: April 2, 2024

Abstract Food security is important for the ever-growing global population. Soybean, Glycine max (L.) Merr., cultivated worldwide providing a key source of food, protein and oil. Hence, it imperative to maintain or increase its yield under different conditions including challenges caused by abiotic biotic stresses. In recent years, soybean pod-sucking stinkbug Riptortus pedestris has emerged as an agricultural insect pest in East, South Southeast Asia. Here, we present genomics resource R. genome assembly, messenger RNA (mRNA) microRNA (miRNA) transcriptomes at developmental stages from organs. As hormone biosynthesis genes (genes involved metamorphosis) their regulators such miRNAs are potential targets control, analyzed sesquiterpenoid (juvenile) ecdysteroid (molting) pathway relevant neuropeptides. Temporal gene expression changes these pathways were observed stages. Similarly, diet-specific response was also both head salivary glands. Furthermore, that microRNAs (bantam, miR-14, miR-316, miR-263) fed with types soybeans differentially expressed glands indicating response. Interestingly, opposite arms miR-281 (-5p -3p), miRNA regulating development, predicted target Hmgs soybean, respectively. These observations among others highlight stinkbug’s responses function interaction soybean. brief, results this study not only salient findings could be use management mitigation but provide invaluable model facilitate studies on plant-pest interactions.

Language: Английский

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Identification and expression patterns of somatic piRNAs and PIWI genes in Riptortus pedestris (Hemiptera: Alydidae)

Archives of Insect Biochemistry and Physiology, Journal Year: 2024, Volume and Issue: 115(4)

Published: April 1, 2024

Abstract RNA interference (RNAi)‐based gene silencing is a feasible and sustainable technology for the management of hemipteran pests by double‐stranded involvement, including small‐interfering RNA, microRNA, Piwi‐interacting (piRNA) pathways, that may help to decrease usage chemical insecticides. However, only few data are available on somatic piRNAs their biogenesis genes in Riptortus pedestris , which serves as significant pest soybean ( Glycine max ). In this study, two family members PIWI were identified characterized R. containing Argonaute3 (Rp Ago3 ) Aubergine Aub with conserved protein domains, clusters validated phylogenetic analysis. addition, they widely expressed all developmental stages whole body had lower expression levels guts under different rearing conditions based previous transcriptome sequencing. Furthermore, abundant clean reads filtered total number 45,998 uridine bias at first nucleotide (nt) position 26–32 nt length mapping onto reference genome according our whole‐transcriptome Finally, revealed gut bacterial changes significantly positively or negatively associated differentially among five comparison groups Pearson correlation conclusion, these findings paved new avenues application RNAi‐based biopesticides broad‐spectrum control.

Language: Английский

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Global climate change‐driven impacts on the Asian distribution of Limassolla leafhoppers, with implications for biological and environmental conservation

Ecology and Evolution, Journal Year: 2024, Volume and Issue: 14(7)

Published: July 1, 2024

Abstract Knowing the impacts of global climate change on habitat suitability distribution Limassolla leafhoppers contributes to understanding feedback organisms from a macroecological perspective, and provides important scientific basis for protecting ecological environment biodiversity. However, there is limited knowledge this aspect. Thus, our study aimed address gap by analyzing Asian centroid shifts based 19 bioclimatic variables occurrence records. Selecting five niche models with outstanding predictive performance (Maxlike, generalized linear model, additive random forest, maximum entropy) along their ensemble model 12 models, current its future under two Shared Socio‐economic Pathways (SSP1‐2.6 SSP5‐8.5) in 2050s 2090s were predicted. The results showed that prediction are generally consistent. Based 11 potential biodiversity hotspots high identified. With change, suitable range will experience both expansion contraction. In SSP5‐8.52050s, area 118.56 × 10 4 km 2 , while contraction 25.40 ; SSP1‐2.62090s, 91.71 26.54 . Furthermore, core shift toward higher latitudes northeast direction, precipitation warmest quarter was found have greatest impact Our research supported four hypotheses. Finally, suggests establishing reserves identified prevent loss, enhancing protection hotspots, pursuing sustainable development path reduced emissions.

Language: Английский

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Functional analysis of neutral lipases in bug feeding and reproduction

Pest Management Science, Journal Year: 2023, Volume and Issue: 79(12), P. 4809 - 4818

Published: July 22, 2023

The bean bug, Riptortus pedestris, is known to cause significant economic losses in soybean crops due its seed-sucking behavior, but the mechanism of adaptation lipid-rich seeds remains poorly understood. To exploit potential target genes for controlling this pest, neutral lipases are functionally characterized study.In study, a total 69 were identified R. including 35 that underwent expansion. phylogeny, expression patterns, and catalytic capacity investigated we selected six salivary gland-specific, eight gut-specific, three ovary-specific functional analysis. All (Chr1.3195, Chr1.0994, Chr5.0087) critical insect reproduction, while few gut-specific (Chr4.0221 Chr1.3207) influence survivorship or weight gain. In contrast, no phenotype change observed when silencing gland-specific lipases.The Chr1.3195, Chr5.0087, Chr4.0221, Chr1.3207 essential pedestris feeding highly sensitive their deficiency, suggesting promising candidates application RNAi-based control destructive pest. © 2023 Society Chemical Industry.

Language: Английский

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