Photoreactivation Activities of Rad5, Rad16A and Rad16B Help Beauveria bassiana to Recover from Solar Ultraviolet Damage DOI Creative Commons

Xin-Cheng Luo,

Lei Yu, Siyuan Xu

et al.

Journal of Fungi, Journal Year: 2024, Volume and Issue: 10(6), P. 420 - 420

Published: June 13, 2024

In budding yeast, Rad5 and Rad7-Rad16 play respective roles in the error-free post-replication repair nucleotide excision of ultraviolet-induced DNA damage; however, their homologs have not yet been studied non-yeast fungi. fungus Beauveria bassiana, a deficiency Rad7 homolog, ortholog two Rad16 paralogs (Rad16A/B) instituted an ability to help insect-pathogenic recover from solar UVB damage through photoreactivation. The fungal lifecycle-related phenotypes were altered absence rad5, rad16A or rad16B, while severe defects growth conidiation caused by double deletion rad16B. Compared with wild-type complemented strains, mutants showed differentially reduced activities regarding resilience UVB-impaired conidia at 25 °C 12-h incubation regime visible light plus dark (L/D 3:9 h 5:7 for photoreactivation) full darkness (dark reactivation) mimicking natural nighttime. estimates median lethal dose LD50 L/D treatments revealed greater Rad16B than Rad16A additive either NER-dependent reactivation photorepair-dependent However, limited recovering low dose-impaired but unable impaired sublethal doses as did photoreactivation 5:7, unless night/dark time was doubled further prolonged. Therefore, anti-UV effects Rad5, B. bassiana depend primarily on are mechanistically distinct those yeast homologs.

Language: Английский

High photoreactivation activities of Rad2 and Rad14 in recovering insecticidal Beauveria bassiana from solar UV damage DOI
Lei Yu, Siyuan Xu,

Xin-Cheng Luo

et al.

Journal of Photochemistry and Photobiology B Biology, Journal Year: 2024, Volume and Issue: 251, P. 112849 - 112849

Published: Jan. 23, 2024

Language: Английский

Citations

2
Divergent roles of Rad4 and Rad23 homologs in Metarhizium robertsii ’s resistance to solar ultraviolet damage DOI

Yilu Zhang,

Peng Han, Ke Zhang

et al.

Applied and Environmental Microbiology, Journal Year: 2023, Volume and Issue: 89(9)

Published: Sept. 1, 2023

The anti-ultraviolet (UV) role of a Rad4-Rad23-Rad33 complex in budding yeast relies on nucleotide excision repair (NER), which is mechanistically distinct from photorepair DNA lesions generated under solar UV irradiation but remains poorly known filamentous fungi. Here, two nucleus-specific Rad4 paralogs (Rad4A and Rad4B) nucleocytoplasmic shuttling Rad23 ortholog are functionally characterized by multiple analyses their null mutants Metarhizium robertsii, an entomopathogenic fungus lacking Rad33. Rad4A was proven to interact with contribute significantly more conidial UVB resistance (90%) than (65%). Despite no other biological function, exhibited very high activity photoreactivation UVB-impaired/inactivated conidia 5-h light exposure due its interaction Rad10, anti-UV protein clarified previously have acquired similar through photolyase M. robertsii. NER or revealed lower reactivation rates moderately impaired after 24-h dark incubation hardly observable at the end 12-h incubation, suggesting infeasibility field where nighttime too short. Aside remarkable contribution resistance, had pleiotropic effect radial growth, aerial conidiation, antioxidant response, cell wall integrity activity. However, Rad4B proved redundant function. unveils essentiality for robertsii's fitness homolog's depending NER. IMPORTANCE Resilience ultraviolet (UV)-impaired cells crucial application fungal insecticides based formulated conidia. Anti-UV roles Rad4, Rad23, Rad33 rely upon (NER) yeast. Among robertsii Rad33, contributes interacts rather Rad4B. acquires severely inactivated another photorepair-required photolyase. either seemingly extant unfeasible conditions. has asexual cycle vitro Therefore, strong depends photoreactivation, unveiling scenario NER-reliant role.

Language: Английский

Citations

5
Rad2, Rad14 and Rad26 recover Metarhizium robertsii from solar UV damage through photoreactivation in vivo DOI
Peng Han,

Yilu Zhang,

Sheng‐Hua Ying

et al.

Microbiological Research, Journal Year: 2023, Volume and Issue: 280, P. 127589 - 127589

Published: Dec. 23, 2023

Language: Английский

Citations

5
Efficient Photoreactivation of Solar UV‐InjuredMetarhizium robertsii by Rad1 and Rad10 Linked to DNA Photorepair‐Required Proteins DOI

Yi‐Lu Zhang,

Peng Han, Sheng‐Hua Ying

et al.

Photochemistry and Photobiology, Journal Year: 2022, Volume and Issue: 99(4), P. 1122 - 1130

Published: Nov. 28, 2022

Abstract Nucleotide excision repair (NER) of ultraviolet (UV)‐induced DNA lesions known as cyclobutane pyrimidine dimer (CPD) and (6–4)‐pyrimidine‐pyrimidone (6‐4PP) photoproducts depends on the activities multiple anti‐UV radiation (RAD) proteins in budding yeast. However, NER remains poorly filamentous fungi, whose are photorepaired by one or two photolyases, namely CPD‐specific Phr1 and/or 6‐4PP‐specific Phr2. Previously, white collar WC1 WC2 were proven to regulate expressions phr2 phr1 photorepair 6‐4PP CDP lesions, respectively, Metarhizium robertsii , a entomopathogenic‐phytoendophytic fungus. We report here high orthologous Rad1 Rad10 5‐h photoreactivation UVB‐injured UVB‐inactivated conidia but severely compromised capability their reactivating those via 24‐h dark incubation M. . The null mutants rad1 rad10 much more conidial UVB resistance than previous wc1 wc2 Multiple protein–protein (Rad1‐Rad10, Rad1‐WC2, Rad10‐Phr1, WC1‐Phr1/2 WC2‐Phr1/2) interactions detected suggest direct/indirect links Phr1/2 WC1/2 an importance for activities. Conclusively, photoreactivate UVB‐impaired through with photorepair‐required proteins.

Language: Английский

Citations

7
Photoreactivation Activities of Rad5, Rad16A and Rad16B Help Beauveria bassiana to Recover from Solar Ultraviolet Damage DOI Creative Commons

Xin-Cheng Luo,

Lei Yu, Siyuan Xu

et al.

Journal of Fungi, Journal Year: 2024, Volume and Issue: 10(6), P. 420 - 420

Published: June 13, 2024

In budding yeast, Rad5 and Rad7-Rad16 play respective roles in the error-free post-replication repair nucleotide excision of ultraviolet-induced DNA damage; however, their homologs have not yet been studied non-yeast fungi. fungus Beauveria bassiana, a deficiency Rad7 homolog, ortholog two Rad16 paralogs (Rad16A/B) instituted an ability to help insect-pathogenic recover from solar UVB damage through photoreactivation. The fungal lifecycle-related phenotypes were altered absence rad5, rad16A or rad16B, while severe defects growth conidiation caused by double deletion rad16B. Compared with wild-type complemented strains, mutants showed differentially reduced activities regarding resilience UVB-impaired conidia at 25 °C 12-h incubation regime visible light plus dark (L/D 3:9 h 5:7 for photoreactivation) full darkness (dark reactivation) mimicking natural nighttime. estimates median lethal dose LD50 L/D treatments revealed greater Rad16B than Rad16A additive either NER-dependent reactivation photorepair-dependent However, limited recovering low dose-impaired but unable impaired sublethal doses as did photoreactivation 5:7, unless night/dark time was doubled further prolonged. Therefore, anti-UV effects Rad5, B. bassiana depend primarily on are mechanistically distinct those yeast homologs.

Language: Английский

Citations

1