Establishment and evaluation of targeted molecular screening model for the ryanodine receptor or sarco/endoplasmic reticulum calcium ATPase

Pest Management Science, Journal Year: 2024, Volume and Issue: 80(7), P. 3369 - 3378

Published: Feb. 23, 2024

Abstract BACKGROUD Endoplasmic reticulum/sarcoplasmic reticulum (ER/SR) is crucial for maintaining intracellular calcium homeostasis due to the calcium‐signaling‐related proteins on its membrane. While ryanodine receptors (RyR) insect ER/SR membranes are well‐known as targets diamide insecticides, little known about other channels. Given resistance of establishment molecular screening models targeting RyR or sarco/endoplasmic ATPase (SERCA) conducive discovery new insecticidal molecules. RESULTS The morphological features Mythimna separata SR have closed vesicles with integrity and high density. 282 in component contained SERCA. A measurement model release uptake was successfully established by detecting ions outside membrane using a fluorescence spectrophotometer. In vitro testing systems found that insecticides could activate dose‐dependently RyR, EC 50 values 0.14 μM (Chlorantraniliprole), 0.21 (Flubendiamide), 0.57 (Cyantraniliprole), respectively. However, dantrolene inhibited RyR‐mediated an IC value 353.9 μM, suggesting can weakly antagonize RyR. Moreover, cyclopiazonic acid significantly reduced enzyme activity capacity On contrary, CDN1163 markedly activated improved transport CONCLUSIONS be used study function unknown membranes, well high‐throughput highly active compounds © 2024 Society Chemical Industry.

Language: Английский

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Risk assessment, cross-resistance, biochemical mechanism and fitness cost of tetraniliprole resistance in the tomato pinworm Tuta absoluta

Crop Protection, Journal Year: 2024, Volume and Issue: 183, P. 106756 - 106756

Published: May 23, 2024

Language: Английский

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The Evolution and Mechanisms of Multiple-Insecticide Resistance in Rice Stem Borer, Chilo suppressalis Walker (Lepidoptera: Crambidae)

Journal of Agricultural and Food Chemistry, Journal Year: 2024, Volume and Issue: unknown

Published: Nov. 18, 2024

The emergence of insecticide resistance in the rice stem borer, Chilo suppressalis, is a growing threat to sustainable control this important insect crop pest. Thus, monitoring C. suppressalis populations for and characterization underlying genetic mechanisms essential inform rational decisions development management strategies. Here, we monitored 126 field from China evolution four major insecticides: 53 chlorantraniliprole, 50 abamectin, 74 triazophos, 76 spinetoram. Moderate high levels were observed all insecticides. Investigation revealed multiple mutations ryanodine receptor (RyR) acetylcholinesterase 1 (AChE1), leading target-site chlorantraniliprole respectively. In contrast, absence glutamate-gated chloride channel (GluCl) α6 nicotinic acetylcholine (nAChR α6) subunit suggested that nontarget site contribute multiple-insecticide phenotypes suppressalis. regard, overexpression uridine 5′-diphospho-glycosyltransferase UGT33AF1 cytochrome P450 CYP6AB45 populations. Functional using transgenic Drosophila demonstrated confers against insecticides vivo, whereas does not appear resistance. Collectively, our findings reveal current status uncover diverse profile species. These provide foundation strategies effectively manage

Language: Английский

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Treatment of Four Stored-Grain Pests with Thiamethoxam plus Chlorantraniliprole: Enhanced Impact on Different Types of Grain Commodities and Surfaces

Insects, Journal Year: 2023, Volume and Issue: 14(7), P. 619 - 619

Published: July 10, 2023

An insecticide containing the neonicotinoid thiamethoxam + diamide chlorantraniliprole was evaluated against adults of Rhyzopertha dominica, Tribolium castaneum, Trogoderma granarium, and Sitophilus oryzae under laboratory bioassays both on freshly treated grain as well stored over 90 days for its persistence in efficacy. In bioassays, applied wheat, maize, or rice at four doses, while wheat same doses. Mortality progeny were assessed bioassays. After 14 exposure, S. exhibited 100% mortality all three commodities highest dose, R. dominica showed complete T. castaneum wheat. For a period days, 42.69% mortality, followed by (35.26%), (27.08%), granarium (18.63%) dose. Progeny successfully suppressed cases Laboratory trials also performed plywood, concrete, ceramic tile, steel one The observed steel, plywood insect species tested. This study demonstrates that is effective tested depending storage period, surface, commodity,

Language: Английский

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Optimization of ultrasound extraction technology of DTD from Ageratina adenophora, and the preparation and characterization of nanoemulsions: Insecticidal activity and safety evaluation

Industrial Crops and Products, Journal Year: 2023, Volume and Issue: 204, P. 117369 - 117369

Published: Aug. 25, 2023

Language: Английский

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Knockdown of the glucosamine-6-phosphate N-acetyltransferase gene by RNA interference enhances the virulence of entomopathogenic fungi against rice leaffolder Cnaphalocrocis medinalis

Pesticide Biochemistry and Physiology, Journal Year: 2024, Volume and Issue: 205, P. 106119 - 106119

Published: Sept. 4, 2024

Insect cuticle acts as a first line of defense and physical protective barrier against entomopathogens. Chitin biosynthesis pathway plays crucial role in chitin formation the insects. Glucosamine-6-phosphate N-acetyltransferase (GNA) is key enzyme insect that regulate formation. However, how GNA-mediated metabolism influences virulence entomopathogenic fungi still unknown. In this study, CmGNA gene was cloned characterized from rice leaffolder Cnaphalocrocis medinalis. The contains an open read frame (ORF) 600 nucleotides, encoding 199 amino acids with isoelectric point 8.65 molecular weight 22.30 kDa. expression profile showed highly expressed 4th instar larvae cuticle. Here, we also reported impact fungi, Metarhizium anisopliae Beauveria bassiana, on pattern genes, feeding behavior, survival rate average body infected larvae, phenotypic deformities, pupation, adult emergence. Our results knockdown application M. B. bassiana three days after RNA interference (RNAi) significantly decreased other associated reduced efficiency rate, caused loss weight, less pupation emergence larvae. Knockdown increased lethality by resulted deformities findings illustrated RNAi-mediated disturbed synthesis genes led to enhancing which can provide us new insights develop novel biocontrol strategies C.

Language: Английский

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Tetraniliprole risk assessment: Unveiling a hidden threat for managing a generalist herbivore

Environmental Research, Journal Year: 2024, Volume and Issue: 256, P. 119273 - 119273

Published: May 29, 2024

Language: Английский

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Phytochemical strategies for combating Spodoptera litura (Fab.): a review of botanicals and their metabolites

Toxin Reviews, Journal Year: 2024, Volume and Issue: 43(4), P. 591 - 633

Published: Aug. 2, 2024

The adverse effects of the lepidopteran pest, Spodoptera litura (Fab.) on crops, result in significant economic losses due to decreased crop productivity and cost pest control measures. Using synthetic chemicals manage this polyphagous can adversely impact environment contribute development pesticide resistance other insects. Integrated management strategies, including biological agents, are often recommended S. infestations a more sustainable environmentally friendly manner. Compared with chemicals, herbal pesticides generally considered nontoxic for environment. They have lower toxicity toward non-target organisms, thus reducing risk harming beneficial animals wildlife. Botanicals include variety phytocompounds that potentially help managing pests, such as litura. Improving herbal-based nano-pesticides (Hb-Np) serve carriers bioactive compounds would enable their controlled targeted release. Therefore, present review aims highlight different mechanisms employed by larvae against efficacy extracts, essential oils, nano-formulations targeting litura, key challenges associated agriculture sector.

Language: Английский

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Physiological Status of Rice Leaf-Roller Cnaphalocrocis medinalis (Lepidoptera: Crambidae) Adults Trapped by Sex Pheromone and Floral Odor

Insects, Journal Year: 2024, Volume and Issue: 15(9), P. 637 - 637

Published: Aug. 25, 2024

The rice leaf-roller Cnaphalocrocis medinalis is an important migratory pest of rice. We conducted a study to determine the physiological status adults trapped by sex pheromone and floral odor. In immigrant group, number males odor was greater than caught trapping. volume testes similar in above two trapping methods but smaller sweep net method. ovary developmental grade, mating rate, matings females trap were higher those net. local breeding pheromones compared with largest group. ovarian significantly group emigrant adult olfactory response low. larger moths eggs laid female immigrants similar, while comparison trap. difference egg hatchability between both breedings not significant.

Language: Английский

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Inheritance of chlorantraniliprole resistance and fitness costs in a field population of Cnaphalocrocis medinalis (Lepidoptera: Pyralidae) in China

Pest Management Science, Journal Year: 2024, Volume and Issue: 81(1), P. 240 - 246

Published: Sept. 20, 2024

Cnaphalocrocis medinalis is one of the major rice insect pests in Asia. Chlorantraniliprole most important insecticides for control C. medinalis. In this study, a field-resistant population and susceptible strain were used to evaluate inheritance chlorantraniliprole resistance fitness costs field.

Language: Английский

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